Aminopeptidase N (rat)
Aminopeptidase N (rat) (APN/CD13) is a Zn2+-dependent membrane-bound exopeptidase that preferentially degrades proteins and peptides with N-terminal neutral amino acids. Aminopeptidase N (rat) is inhibited by angiotensin IV and participates in the regulation of angiotensin IV half-life in the rat striatum.
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- CAS No.: 9054-63-1
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Storage:
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Aminopeptidase N (rat) accounted for 60 % of the total aminopeptidase activity in rat striatal cell membranes[1].
Aminopeptidase N (rat)'s over-expression reduces basolateral Na+/K+ ATPase activity and abundance in LLCPK-1 proximal tubule cells, with this effect dependent on signaling via the AT4 receptor[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Aminopeptidase N (rat) (intracerebroventricular infusion; microinfusion into paraventricular nucleus of the hypothalamus) reduces arterial blood pressure in both spontaneously hypertensive and normotensive rats, with a more pronounced effect in spontaneously hypertensive rats, via an angiotensin type 1 receptor-dependent mechanism[2].
Aminopeptidase N (rat) has increased renal abundance and activity in salt-resistant rat strains fed a high-salt diet, supporting a role in renal salt adaptation and blood pressure regulation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (male, 250-300 g)[1]
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Dosage:10 nM, 100 nM, 500 nM
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Administration:local perfusion via microdialysis probe; 60 min
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Result:Caused no significant change in extracellular dopamine concentrations in the rat striatum, with levels remaining near baseline (100%) throughout and after perfusion (10 nM, 100 nM, 500 nM inhibitor 7B alone).
Potentiated the angiotensin IV-induced increase in striatal dopamine levels, resulting in a maximal increase of approximately 350% of baseline at 60 min of co-perfusion.
Completely abolished the angiotensin IV-induced dopamine increase, with dopamine levels remaining at baseline (500 nM inhibitor 7B + 10 µM angiotensin IV, combined APN and IRAP inhibition).
Chemical Information
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CAS No. 9054-63-1
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SMILES
[Aminopeptidase N (rat)]
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Synonyms
APN/CD13
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Stragier B, et al. Involvement of insulin-regulated aminopeptidase and/or aminopeptidase N in the angiotensin IV-induced effect on dopamine release in the striatum of the rat. Brain Res. 2007;1131(1):97-105. [Content Brief]
[2]. Danziger RS, et al. Aminopeptidase N in arterial hypertension. Heart Fail Rev. 2008;13(3):293-298. [Content Brief]
[3]. Sjöström H, et al. Structure and function of aminopeptidase N. Adv Exp Med Biol. 2000;477:25-34. [Content Brief]
[4]. Wickström M, et al. Aminopeptidase N (CD13) as a target for cancer chemotherapy. Cancer Sci. 2011;102(3):501-508. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Aminopeptidase N (rat) Related Classifications
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