Benoxacor

Benoxacor (5 μM; 0-30 min) is metabolized by liver microsomes from male C57BL/6 mice in a time-dependent and enantioselective manner. Cytochrome P450 enzymes primarily metabolize the E2-benoxacor enantiomer, while microsomal carboxylesterases mainly metabolize the E1-benoxacor enantiomer. The intrinsic clearance of microsomal CYP-mediated elimination is 1.05 mL/min/mg protein, with a normalized clearance of 40.22 mL/min/g liver, whereas the intrinsic clearance of microsomal CES-mediated elimination is 0.54 mL/min/mg protein, with a normalized clearance of 20.77 mL/min/g liver^[1].
Benoxacor (5 μM; 0-30 min) is metabolized in a time-dependent and enantioselective manner by glutathione S-transferase in the liver cytosol of male C57BL/6 mice, with an intrinsic clearance rate of 1.46 mL/min/mg protein and a normalized clearance rate of 132.52 mL/min/g liver for GST-mediated elimination^[1].
Benoxacor (10 μM; 0.25-48 h) is rapidly taken up by suspension-cultured cells of maize (cultivar: Black Mexican Sweet, same below), and the uptake amount increases continuously within 48 h after treatment with 10 μM^[3].
Treatment of maize suspension-cultured cells with Benoxacor (10 μM; 0-48 h) doubles the activity of GST-M, a change detectable as early as 4 h post-treatment and peaking at 24 h^[3].
Benoxacor (10 μM; 24 h) increases the activities of three distinct GSTs (a, c, d) isolated via anion exchange chromatography from treated maize suspension-cultured cells^[3].
Isolation of GST fractions (b, c, d) from maize suspension-cultured cells treated with Benoxacor (10 μM; 24 h) reveals GST polypeptides (26,000-27,000 M_r) that cross-react with maize GST I and GST I/III antisera, a pattern consistent with that observed in benzofenap-treated maize seedlings^[3].
Treatment of maize suspension-cultured cells with Benoxacor (10 μM; 24 h) increases GST-M activity by 3-fold, and this effect is partially blocked by pre-treatment with Cycloheximide (HY-12320) (10/100 μM; 2 h), indicating that this induction activity requires de novo protein synthesis^[3].
Treatment of maize suspension-cultured cells with Benoxacor (10 μM; 24 h) increases GST-M activity by 3-fold, and this enhancing effect is significantly blocked by pretreatment with Cordycepin (HY-N0262) (10/100 μM; 2 h), indicating that this inductive activity requires de novo RNA synthesis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.