Cordycepin (Synonyms: 3'-Deoxyadenosine)

Name: Cordycepin
Brand: MedChemExpress
SKU: HY-N0262
Rating: 5.0 (13 reviews)

Cat. No.: HY-N0262 Purity: 99.80%: Data Sheet
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Cordycepin (3'-Deoxyadenosine) is a nucleoside derivative and inhibits IL-1β-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts (RASFs) in a dose-dependent manner. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase.

For research use only. We do not sell to patients.

CAS No. : 73-03-0

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Cordycepin:

Cordycepin (Standard) In-stock

13 Publications Citing Use of MCE Cordycepin

Cell Imaging/Staining

RT-PCR

Flow Cytometry

Cell Proliferation/Viability Assay

: Cordycepin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Jun 10;10(1):25 [Abstract]; Western blot analysis of p16 and p21 levels in irradiated control or Cordycepin (200 μM)-treated fibroblasts 7 days after radiation.

: Cordycepin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Jun 10;10(1):25 [Abstract]; Treated skin fibroblasts with 200 μM Cordycepin for 3 days before irradiation and found cordycepin-treated fibroblasts exposed to radiation showed reduced levels of the DNA damage response marker γ-H2AX at early and late time points.

: Cordycepin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Jun 10;10(1):25 [Abstract]; Quantification of mRNA expression for senescence secretory phenotype in irradiated control or Cordycepin (200 μM)-treated fibroblasts 7 days after radiation.

: Cordycepin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Jun 10;10(1):25 [Abstract]; Fibroblasts were treated with Cordycepin (200 μM) for 72 h and harvested for detection of apoptosis using flow cytometry 24 h following exposure to radiation.

: Cordycepin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Jun 10;10(1):25 [Abstract]; Cordycepin (200 μM) increased the colony forming efficiency of irradiated fibroblasts and HaCaT cells.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Cordycepin (3'-Deoxyadenosine) is a nucleoside derivative and inhibits IL-1β-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts (RASFs) in a dose-dependent manner^[1]. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase^[2].

IC₅₀ & Target^[1]

MMP-1

MMP-3

Cellular Effect

Cell Line	Type	Value	Description	References
5637	IC₅₀	71.56 μM Compound: 10	Cytotoxicity against human 5637 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human 5637 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
A498	IC₅₀	85.36 μM Compound: 10	Cytotoxicity against human A498 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human A498 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
CAKI-2	IC₅₀	94.2 μM Compound: 10	Cytotoxicity against human CAKI-2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human CAKI-2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
COLO 205	IC₅₀	107.93 μM Compound: 10	Cytotoxicity against human COLO 205 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human COLO 205 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
HL-60	IC₅₀	145.24 μM Compound: 10	Cytotoxicity against human HL-60 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human HL-60 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
HT-29	IC₅₀	26.33 μM Compound: 10	Cytotoxicity against human HT-29 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human HT-29 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
HepG2	IC₅₀	77.19 μM Compound: 10	Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
K562	IC₅₀	94.44 μM Compound: 10	Cytotoxicity against human K562 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human K562 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
KG-1	IC₅₀	88.96 μM Compound: 10	Cytotoxicity against human KG-1 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human KG-1 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
L929	IC₅₀	28.7 μM Compound: 1a, Cordycepin	Cytotoxicity against mouse L929 cells assessed as cell viability after 72 hrs by WST-1 assay Cytotoxicity against mouse L929 cells assessed as cell viability after 72 hrs by WST-1 assay	[PMID: 24283924]
MCF7	IC₅₀	45.88 μM Compound: 10	Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
MDA-MB-231	IC₅₀	78.63 μM Compound: 10	Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
MIA PaCa-2	IC₅₀	76.78 μM Compound: 10	Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
MOLT-4	IC₅₀	0.1 μM Compound: 1a, Cordycepin	Cytotoxicity against human MOLT4 cells assessed as cell viability after 72 hrs by WST-1 assay in presence of deoxycoformycin Cytotoxicity against human MOLT4 cells assessed as cell viability after 72 hrs by WST-1 assay in presence of deoxycoformycin	[PMID: 24283924]
MOLT-4	IC₅₀	85.28 μM Compound: 10	Cytotoxicity against human MOLT-4 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human MOLT-4 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
MOLT-4	IC₅₀	>= 100 μM Compound: 1a, Cordycepin	Cytotoxicity against human MOLT4 cells assessed as cell viability after 72 hrs by WST-1 assay Cytotoxicity against human MOLT4 cells assessed as cell viability after 72 hrs by WST-1 assay	[PMID: 24283924]
MT4	ED₅₀	> 50 μM Compound: 2f	Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells. Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells.	[PMID: 3172142]
PC-3	IC₅₀	85.34 μM Compound: 10	Cytotoxicity against human PC-3 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human PC-3 cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
RL	IC₅₀	135.06 μM Compound: 10	Cytotoxicity against human RL cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay Cytotoxicity against human RL cells assessed as inhibition of cell viability incubated for 72 hrs by MTS assay	[PMID: 38547648]
Vero	IC₅₀	0.5 nM Compound: Cordycepin	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]

In Vitro

Cordycepin is a potent inhibitor of IL-1β-induced chemokine production and MMP expression and strongly blocks the p38/JNK/AP-1 signalling pathway in RASFs. The effect of Cordycepin on cellular toxicity of RASFs is assessed using MTT assay. Treatment of RASFs with Cordycepin (50 μM or 100 μM) for 24 h does not cause any significant change in cell viability. However, cell viability is slightly decreased when cells are incubated with 100 μM Cordycepin for 48 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

251.24

Formula

C₁₀H₁₃N₅O₃

CAS No.

73-03-0

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1C[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O1

Structure Classification

Others

Initial Source

Microorganisms

Cordyceps militaris

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (132.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2 mg/mL (7.96 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9803 mL	19.9013 mL	39.8026 mL
5 mM	0.7961 mL	3.9803 mL	7.9605 mL
10 mM	0.3980 mL	1.9901 mL	3.9803 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 5.56 mg/mL (22.13 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 12.5 mg/mL (49.75 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (290 KB) SDS (420 KB)

COA (251 KB) HNMR (134 KB) RP-HPLC (226 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. Noh EM, et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford). 2009 Jan;48(1):45-8. [Content Brief]

[2]. Huang F, et al. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase. PLoS One. 2019 Jun 14;14(6):e0218449. [Content Brief]

Cell Assay
^[1]

RASFs (2×10⁴ cells/well) are treated with various concentrations of Cordycepin (50 μM or 100 μM). After incubation for 1 h, 12 h and 24 h, cells are washed twice with PBS, MTT (0.5 mg/mL PBS) is added to each well and incubated at 37°C for 30 min. Formazan crystals formed are dissolved by adding DMSO (100 μL/well) and the absorbance is read at 570 nm using a microplate reader^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Noh EM, et al. Cordycepin inhibits IL-1beta-induced MMP-1 and MMP-3 expression in rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford). 2009 Jan;48(1):45-8. [Content Brief]

[2]. Huang F, et al. Cordycepin kills Mycobacterium tuberculosis through hijacking the bacterial adenosine kinase. PLoS One. 2019 Jun 14;14(6):e0218449. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.9803 mL	19.9013 mL	39.8026 mL	99.5064 mL
H₂O / DMSO	5 mM	0.7961 mL	3.9803 mL	7.9605 mL	19.9013 mL
DMSO	10 mM	0.3980 mL	1.9901 mL	3.9803 mL	9.9506 mL
	15 mM	0.2654 mL	1.3268 mL	2.6535 mL	6.6338 mL
	20 mM	0.1990 mL	0.9951 mL	1.9901 mL	4.9753 mL
	25 mM	0.1592 mL	0.7961 mL	1.5921 mL	3.9803 mL
	30 mM	0.1327 mL	0.6634 mL	1.3268 mL	3.3169 mL
	40 mM	0.0995 mL	0.4975 mL	0.9951 mL	2.4877 mL
	50 mM	0.0796 mL	0.3980 mL	0.7961 mL	1.9901 mL
	60 mM	0.0663 mL	0.3317 mL	0.6634 mL	1.6584 mL
	80 mM	0.0498 mL	0.2488 mL	0.4975 mL	1.2438 mL
	100 mM	0.0398 mL	0.1990 mL	0.3980 mL	0.9951 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cordycepin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cordycepin73-03-03'-DeoxyadenosineTNF ReceptorMMPBacterialAutophagyAntibioticTumor Necrosis Factor ReceptorTNFRMatrix metalloproteinasesInhibitorinhibitorinhibit

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Cordycepin (Synonyms: 3'-Deoxyadenosine)

Customer Review

Other Forms of Cordycepin:

Cordycepin Related Antibodies

13 Publications Citing Use of MCE Cordycepin

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Cordycepin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cordycepin Related Classifications

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