Cat. No.: HY-N1098

Velutin

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Velutin is a flavonoid. Velutin can be extracted from mistletoe. Velutin inhibits mushroom Tyrosinase activity with an IC₅₀ of 910.1 μM. Velutin inhibits p38 phosphorylation, the NF-κB pathway and the MAPK pathway. Velutin prevents articular cartilage degeneration and subchondral bone loss. Velutin slows down the progression of intervertebral disc degeneration. Velutin exhibits inhibitory effects on melanogenesis, skin whitening, anti-inflammatory, anti-allergic, anti-oxidant and antibacterial activities. Velutin can be used in studies related to pigmented diseases, osteoarthritis and intervertebral disc degeneration.

For research use only. We do not sell to patients.

Purity: 98.60%
CAS No.: 25739-41-7
Formula: C₁₇H₁₄O₆
Molecular Weight:314.29

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Velutin Chemical Structure

CAS No.: 25739-41-7

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(In Japan)

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All p38 MAPK Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	>20 μg/mL Compound: 4',5-dihydroxy-3',7-dimethoxyflavone	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 15217279]
KB	IC₅₀	1.5 μg/mL Compound: Velutin	Cytotoxicity against human KB cells after 72 hrs by neutral red assay Cytotoxicity against human KB cells after 72 hrs by neutral red assay	[PMID: 10075750]
KB	IC₅₀	1.5 μg/mL Compound: 4, Velutin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8759170]
Neutrophil	IC₅₀	<=12.51 μM Compound: 59	Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion generation pre-incubated for 2 mins prior to CB induction for 3 mins followed by fMLF addition by spectrophotometric analysis Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion generation pre-incubated for 2 mins prior to CB induction for 3 mins followed by fMLF addition by spectrophotometric analysis	[PMID: 37683361]
Neutrophil	IC₅₀	<=15.25 μM Compound: 59	Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in elastase release using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate pre-incubated for 2 mins followed by fMLF/CB-stimulation and measured for 5 mins Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in elastase release using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate pre-incubated for 2 mins followed by fMLF/CB-stimulation and measured for 5 mins	[PMID: 37683361]
P388	IC₅₀	10 μg/mL Compound: 4, Velutin	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 8759170]

In Vitro

Velutin (2-200 μM; 10 min) potently scavenges ABTS free radicals in cell-free assays, with an EC₅₀ value of 23.13 μM^[1].
Velutin (0.01-1 mM; pre-incubated with tyrosinase for 30 min, followed by incubation with L-tyrosine for 10 min) inhibits mushroom tyrosinase activity in cell-free assays, with an IC₅₀ of 910.1 μM^[1].
Velutin (10 μM; 48 h) completely inhibits α-MSH-induced melanogenesis in B16F10 mouse melanoma cells^[1].
At a concentration of 10 μM with 48 h incubation, velutin inhibits α-MSH-induced intracellular tyrosinase activity by 87% in B16F10 mouse melanoma cells^[1].
Velutin (0-32 μM; 24-48 h) exhibits no cytotoxicity toward primary mouse chondrocytes, with the maximum concentration reaching 32 μM after 24 h or 48 h of incubation^[2].
Velutin (32 μM; 7 days) protects primary mouse chondrocytes against IL-1β-induced inflammation and extracellular matrix degradation after 7 days of incubation^[2].
Velutin (4 μM; 2 h) inhibits RANKL-induced activation of p38 MAPK in mouse bone marrow-derived macrophages^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

B16F10 murine melanoma cells
Concentration:

20 μM
Incubation Time:

24 h
Result:

Showed no significant cytotoxicity, with cell viability remaining near 100% at 20 μM.

In Vivo

Velutin (32 μM; intra-articular injection; once weekly for 8 consecutive weeks) exerts dual protective effects against articular cartilage degeneration and subchondral bone loss in DMM-induced osteoarthritis mice, reduces the OARSI score, increases the expression of aggrecan and type Ⅱ collagen, and inhibits the activation of p-p38 MAPK^[2].
Velutin (i.p.; once daily; for 4 consecutive weeks; 5-10 mg/kg) dose-dependently alleviates intervertebral disc degeneration in a mouse model of lumbar instability^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (7-week-old male, DMM-induced osteoarthritis)^[2]
Dosage:

32 μM
Administration:

intra-articular injection; once weekly; 8 weeks
Result:

Showed significantly more regular articular cartilage surfaces, a thicker AC layer, a thinner calcified cartilage layer, and a lower osteophyte score.
Increased AC thickness, reduced CC thickness, and increased the AC/CC ratio.
Increased subchondral bone thickness, and significantly reduced the number of TRAP (+) multinucleated osteoclasts in SCB.
Increased aggrecan and type II collagen expression.
Reduced AC destruction and deeper Safranin O staining, with lower Osteoarthritis Research Society International scores.
Increased trabecular bone volume/tissue volume and trabecular number, and reduced trabecular separation in SCB, reducing SCB loss.
Significantly reduced p-p38 MAPK expression.

Chemical Information

CAS No. 25739-41-7
Appearance Solid
Molecular Weight 314.29
Formula C₁₇H₁₄O₆
Color White to yellow
SMILES

O=C1C=C(C2=CC=C(O)C(OC)=C2)OC3=CC(OC)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
- Microorganisms
Flammulina velutipes
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (159.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1818 mL

15.9089 mL

31.8177 mL

5 mM

0.6364 mL

3.1818 mL

6.3635 mL

10 mM

0.3182 mL

1.5909 mL

3.1818 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jung SH, et al. Anti-Melanogenic Properties of Velutin and Its Analogs. Molecules. 2021;26(10):3033. Published 2021 May 19. [Content Brief]

[2]. Wang K, et al. Dual protective role of velutin against articular cartilage degeneration and subchondral bone loss via the p38 signaling pathway in murine osteoarthritis. Front Endocrinol (Lausanne). 2022;13:926934. Published 2022 Jul 22. [Content Brief]

[3]. Zhang Y, et al. Velutin Inhibits IL-1β-Induced Nucleus Pulposus Inflammatory and Extracellular Matrix Degradation Attenuating Mouse Intervertebral Disc Degeneration via the NF-κB and MAPK Pathways. Mediators Inflamm. 2025 Nov 20;2025:9625485. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1818 mL	15.9089 mL	31.8177 mL	79.5444 mL
	5 mM	0.6364 mL	3.1818 mL	6.3635 mL	15.9089 mL
	10 mM	0.3182 mL	1.5909 mL	3.1818 mL	7.9544 mL
	15 mM	0.2121 mL	1.0606 mL	2.1212 mL	5.3030 mL
	20 mM	0.1591 mL	0.7954 mL	1.5909 mL	3.9772 mL
	25 mM	0.1273 mL	0.6364 mL	1.2727 mL	3.1818 mL
	30 mM	0.1061 mL	0.5303 mL	1.0606 mL	2.6515 mL
	40 mM	0.0795 mL	0.3977 mL	0.7954 mL	1.9886 mL
	50 mM	0.0636 mL	0.3182 mL	0.6364 mL	1.5909 mL
	60 mM	0.0530 mL	0.2651 mL	0.5303 mL	1.3257 mL
	80 mM	0.0398 mL	0.1989 mL	0.3977 mL	0.9943 mL
	100 mM	0.0318 mL	0.1591 mL	0.3182 mL	0.7954 mL

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Velutin

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All p38 MAPK Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Velutin Related Classifications

Cat. No.: HY-N1098