2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. BCI

BCI ((E)-BCI) is a DUSP6 (dual specificity phosphatase 6) inhibitor. BCI shows anti-inflammatory activity and decreases reactive oxygen species (ROS) production. BCI can be used in inflammatory disease research.

For research use only. We do not sell to patients.

BCI Chemical Structure

BCI Chemical Structure

CAS No. : 1245792-51-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 116 In-stock
Solution
10 mM * 1 mL in DMSO USD 116 In-stock
Solid
1 mg USD 66 In-stock
5 mg USD 165 In-stock
10 mg USD 275 In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of BCI:

BCI Related Antibodies

Top Publications Citing Use of Products

    BCI purchased from MedChemExpress. Usage Cited in: Kaohsiung J Med Sci. 2022 Dec 16.  [Abstract]

    BCI (8 μM) reverses FAM3D-induced inhibition of gluconeogenesis and expression of DUSP1 in HepG2 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BCI ((E)-BCI) is a DUSP6 (dual specificity phosphatase 6) inhibitor. BCI shows anti-inflammatory activity and decreases reactive oxygen species (ROS) production. BCI can be used in inflammatory disease research[1][2].

    In Vitro

    BCI (100 ng/mL; 24 h) downregulats the expression of DUSP6 in RAW264.7 macrophage cells[2].
    ? BCI (0-1 nM; 24 h) inhibits the expression of IL-1β and IL-6 in lipopolysaccharide- (LPS-) activated macrophages[2].
    ? BCI (0-4 nM; 24 h) decreases ROS production and activates the Nrf2 Pathway in LPS-activated macrophages[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BCI Related Antibodies

    Western Blot Analysis[2]

    Cell Line: RAW264.7 macrophage cells
    Concentration: 100 ng/mL
    Incubation Time: 24 hours
    Result: Showed DUSP6 protein downregulation.

    RT-PCR[2]

    Cell Line: RAW264.7 macrophage cells
    Concentration: 0-1 nM
    Incubation Time: 24 hours
    Result: Inhibited the expression of IL-1β and IL-6 mRNA in LPS-activated macrophages.
    Molecular Weight

    317.43

    Formula

    C22H23NO
    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=C1/C(C(NC2CCCCC2)C3=C1C=CC=C3)=C/C4=CC=CC=C4
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (393.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1503 mL 15.7515 mL 31.5030 mL
    5 mM 0.6301 mL 3.1503 mL 6.3006 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (6.55 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (6.55 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1503 mL 15.7515 mL 31.5030 mL 78.7575 mL
    5 mM 0.6301 mL 3.1503 mL 6.3006 mL 15.7515 mL
    10 mM 0.3150 mL 1.5752 mL 3.1503 mL 7.8758 mL
    15 mM 0.2100 mL 1.0501 mL 2.1002 mL 5.2505 mL
    20 mM 0.1575 mL 0.7876 mL 1.5752 mL 3.9379 mL
    25 mM 0.1260 mL 0.6301 mL 1.2601 mL 3.1503 mL
    30 mM 0.1050 mL 0.5251 mL 1.0501 mL 2.6253 mL
    40 mM 0.0788 mL 0.3938 mL 0.7876 mL 1.9689 mL
    50 mM 0.0630 mL 0.3150 mL 0.6301 mL 1.5752 mL
    60 mM 0.0525 mL 0.2625 mL 0.5251 mL 1.3126 mL
    80 mM 0.0394 mL 0.1969 mL 0.3938 mL 0.9845 mL
    100 mM 0.0315 mL 0.1575 mL 0.3150 mL 0.7876 mL
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    BCI Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BCI1245792-51-1(E)-BCIPhosphataseanti-inflammatory activityRAW264.7 macrophage cellInhibitorinhibitorinhibit

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