BCI hydrochloride (Synonyms: (E)-BCI hydrochloride)

Cat. No.: HY-115502A Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

BCI ((E)-BCI) hydrochloride is a DUSP6 (dual specificity phosphatase 6) inhibitor. BCI hydrochloride shows anti-inflammatory activity and decreases reactive oxygen species (ROS) production. BCI hydrochloride can be used in inflammatory disease research.

For research use only. We do not sell to patients.

BCI hydrochloride Chemical Structure

CAS No. : 95130-23-7

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 109	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 109	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of BCI hydrochloride:

BCI In-stock
(E/Z)-BCI Get quote

6 Publications Citing Use of MCE BCI hydrochloride

: BCI hydrochloride purchased from MCE. Usage Cited in: Kaohsiung J Med Sci. 2022 Dec 16. [Abstract]; BCI (8 μM) reverses FAM3D-induced inhibition of gluconeogenesis and expression of DUSP1 in HepG2 cells.

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Description

IC₅₀ & Target

DUSP6^[1]

In Vitro

BCI (100 ng/mL; 24 h) downregulats the expression of DUSP6 in RAW264.7 macrophage cells^[2].
BCI (0-1 nM; 24 h) inhibits the expression of IL-1β and IL-6 in lipopolysaccharide- (LPS-) activated macrophages^[2].
BCI (0-4 nM; 24 h) decreases ROS production and activates the Nrf2 Pathway in LPS-activated macrophages^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	100 ng/mL
Incubation Time:	24 hours
Result:	Showed DUSP6 protein downregulation.

RT-PCR^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	0-1 nM
Incubation Time:	24 hours
Result:	Inhibited the expression of IL-1β and IL-6 mRNA in LPS-activated macrophages.

Molecular Weight

353.89

Appearance

Solid

Formula

C₂₂H₂₄ClNO

CAS No.

95130-23-7

SMILES

O=C1/C(C(NC2CCCCC2)C3=C1C=CC=C3)=C/C4=CC=CC=C4.Cl.[(E)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 15.62 mg/mL (44.14 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8257 mL	14.1287 mL	28.2574 mL
5 mM	0.5651 mL	2.8257 mL	5.6515 mL
10 mM	0.2826 mL	1.4129 mL	2.8257 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (285 KB) SDS (251 KB)

COA (261 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Korotchenko VN, et al. In vivo structure-activity relationship studies support allosteric targeting of a dual specificity phosphatase. Chembiochem. 2014 Jul 7;15(10):1436-45. [Content Brief]

[2]. Zhang F, et al. DUSP6 Inhibitor (E/Z)-BCI Hydrochloride Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Murine Macrophage Cells via Activating the Nrf2 Signaling Axis and Inhibiting the NF-κB Pathway. Inflammation. 2019 Apr;42(2):672-681. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

BCI95130-23-7(E)-BCIPhosphataseanti-inflammatory activityRAW264.7 macrophage cellInhibitorinhibitorinhibit

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BCI hydrochloride (Synonyms: (E)-BCI hydrochloride)

Customer Review

Other Forms of BCI hydrochloride:

6 Publications Citing Use of MCE BCI hydrochloride

Featured Recommendations

•Related Screening Libraries:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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