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BTB09089 is a T cell death-associated gene 8 (TDAG8/GPR65) specific agonist. BTB09089 increases TDAG8 expression and regulates the cytokine production of T cells and macrophages.

For research use only. We do not sell to patients.

BTB09089 Chemical Structure

BTB09089 Chemical Structure

CAS No. : 245728-44-3

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5 mg USD 270 In-stock
10 mg USD 480 In-stock
25 mg USD 880 In-stock
50 mg USD 1320 In-stock
100 mg USD 1980 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

BTB09089 is a T cell death-associated gene 8 (TDAG8/GPR65) specific agonist. BTB09089 increases TDAG8 expression and regulates the cytokine production of T cells and macrophages[1].

IC50 & Target

TDAG8/GPR65[1]

In Vitro

BTB09089 (0-18 μM; 30 min) significantly increases cAMP accumulation in hTDAG8 and mTDAG8 transiently expressing HEK293 cells, but not in control HEK 293 cells. BTB09089 does not increase cAMP accumulation in hGPR4 expressing HEK293 cells nor does it increase inositol phosphate accumulation in hOGR1 expressing HEK293 cells[1].
BTB09089 (0-18 μM; 30 min) significantly enhances the cAMP accumulation in a dose-dependent manner at pH 7.0-7.9 but not at pH 6.5[1].
BTB09089 (1-5 μM; 20 h) suppresses IL-2 production in splenocytes from WT mice in a dose-dependent manner but not from TDAG8 KO mice without affecting the cell viability[1].
BTB09089 (1-5 μM; 18 h) suppresses LPS (HY-D1056)-stimulated TNF-α and IL-6 production, and enhances LPS-stimulated IL-10 production in thioglycollate induced peritoneal exude cells (TG-PEC)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

BTB09089 (5-20 μM, 8 μL; i.c.v.; 6 hours prior to MCAO) reduces cerebral ischaemia-reperfusion injury in rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male SD rats (270-280 g), middle cerebral artery occlusion (MCAO) model[2]
Dosage: 5, 10 and 20 μM, 8 μL
Administration: Intracerebroventricular injection, six hours prior to MCAO
Result: Up-regulated TDAG8 and Bcl-2 expression and down-regulated cleaved caspase-3 expression, while the infarction volume was reduced, and neurological deficits were ameliorated 24 and 72 h after MCAO.
Molecular Weight

387.31

Formula

C14H12Cl2N4OS2

CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1C2=C(C=NN1C)N(C)N=C(SCC3=CC=C(C=C3Cl)Cl)S2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (258.19 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5819 mL 12.9096 mL 25.8191 mL
5 mM 0.5164 mL 2.5819 mL 5.1638 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (12.91 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
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Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5819 mL 12.9096 mL 25.8191 mL 64.5478 mL
5 mM 0.5164 mL 2.5819 mL 5.1638 mL 12.9096 mL
10 mM 0.2582 mL 1.2910 mL 2.5819 mL 6.4548 mL
15 mM 0.1721 mL 0.8606 mL 1.7213 mL 4.3032 mL
20 mM 0.1291 mL 0.6455 mL 1.2910 mL 3.2274 mL
25 mM 0.1033 mL 0.5164 mL 1.0328 mL 2.5819 mL
30 mM 0.0861 mL 0.4303 mL 0.8606 mL 2.1516 mL
40 mM 0.0645 mL 0.3227 mL 0.6455 mL 1.6137 mL
50 mM 0.0516 mL 0.2582 mL 0.5164 mL 1.2910 mL
60 mM 0.0430 mL 0.2152 mL 0.4303 mL 1.0758 mL
80 mM 0.0323 mL 0.1614 mL 0.3227 mL 0.8068 mL
100 mM 0.0258 mL 0.1291 mL 0.2582 mL 0.6455 mL
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BTB09089 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
BTB09089
Cat. No.:
HY-149164
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