Cat. No.: HY-101878

Texas Red

(Synonyms: Sulforhodamine 101; SR101)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.

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Pureté: 99.72%
CAS No.: 60311-02-6
Formule: C₃₁H₃₀N₂O₇S₂
Masse moléculaire:606.71

Stockage:

-20°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Texas Red Chemical Structure

CAS No.: 60311-02-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- In Vivo
- Emission (Em)
- Excitation (Ex)
- Dyeing Example
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
Protocole
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

In Vitro

Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence^[3].
For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34°C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

605

Excitation (Ex)

586

Dyeing Example

Description: Texas Red (Sulforhodamine 101) can be used as an astrocyte marker for hippocampal slices staining with red fluorescence.
Method: For cell staining.
1. Stain hippocampal slices with Texas Red (5 μM; 35 min; 34◦C) in carbogenated Artificial CerebroSpinal Fluid (ACSF).
2. Return hippocampal slices to dye-free ACSF for at least 30 min before imaging.
3. Use a confocal laser scanning microscopy (Zeiss LSM 880) for image.

Chemical Information

CAS No. 60311-02-6
Appearance Solid
Masse moléculaire 606.71
Formule C₃₁H₃₀N₂O₇S₂
Color Green to dark green
SMILES

O=S(C1=CC=C(C(C2=CC3=C4N(CCC3)CCCC4=C2[O+]=C56)=C5C=C7CCCN8CCCC6=C87)C(S(=O)([O-])=O)=C1)(O)=O
Synonyms

Sulforhodamine 101; SR101
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (8)

All Bio/Physico-chemical AssayCell Imaging/Staining

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Journal Impact Factor

Nat Neurosci

Amyloid-β-driven glymphatic dysfunction in Alzheimer's disease model mice is driven by Ca2+-mediated increases in astrocytic cholesterol. [Abstract]

2026 Jun;29(6):1369-1385. PMID: 41986732
Neuron

Astrocytic Piezo1-mediated mechanotransduction determines adult neurogenesis and cognitive functions. [Abstract]

2022 Sep 21;110(18):2984-2999.e8. PMID: 35963237

Texas Red purchased from MedChemExpress. Usage Cited in: Neuron. 2022 Sep 21;110(18):2984-2999.e8. [Abstract]

The astrocyte-specific dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 15 min; 37 ℃) was used for two-photon Ca²⁺ imaging of hippocampal slices.
Adv Healthc Mater

Spiky Nanoparticle-Gel Composite for Efficient Intravesical Botulinum Toxin A Delivery and Treatment of Overactive Bladder. [Abstract]

2026 Mar 27:e05843. PMID: 41889245
Proc Natl Acad Sci U S A

The influence of electrical charge on plasmodesma conductivity. [Abstract]

2026 Apr 14;123(15):e2527879123. PMID: 41941627
RSC Adv

Incubation of brain organoids with fluorescent carbon nanodots. [Abstract]

2026 Apr 8;16(21):18745-18756. PMID: 41959546
Glia

Astroglial N-myc downstream-regulated gene 2 protects the brain from cerebral edema induced by stroke. [Abstract]

2021 Feb;69(2):281-295. PMID: 32652708

Texas Red purchased from MedChemExpress. Usage Cited in: Glia. 2021 Feb;69(2):281-295. [Abstract]

Representative fluorescence images showed Na⁺-insensitive fluorescent dye Texas Red (Sulforhodamine 101; SR101) (1 μM; 30 min; 37 ℃) trapped inside astroglial cytoplasm and counterstained with Hoechst 33342.
Neuropharmacology

Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. [Abstract]

2022 Oct 1:217:109191. PMID: 35835213

Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191. [Abstract]

Example image of a patch-clamp recorded astrocyte labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) in the alveus of the hippocampal CA1 region.

Texas Red purchased from MedChemExpress. Usage Cited in: Neuropharmacology. 2022 Oct 1:217:109191. [Abstract]

Example images of astrocytes in hippocampal slices labeled with Texas Red (Sulforhodamine 101; SR101) (5 μM; 35 min; 34 ℃) and filled with Fluo-4, a calcium indicator. Arrows indicate astrocytes co-labeled with Texas Red (Sulforhodamine 101; SR101) and Fluo-4.
J Biomed Res

A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging. [Abstract]

2022 Aug 10;36(5):358-367. PMID: 36130733

Texas Red purchased from MedChemExpress. Usage Cited in: J Biomed Res. 2022 Aug 10;36(5):358-367. [Abstract]

The trajectory of astrocytic Ca²⁺ signal-weighted centroid from C57BL/6J mice. The grey cellular surface was reconstructed from Texas Red (Sulforhodamine 101; SR101) (5 mM; i.v.; 1 h) labeling. The overlapped red trace was the projection of signal centroid.

Solvant et solubilité

In Vitro:

DMSO : 31.82 mg/mL (52.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 8.33 mg/mL (13.73 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6482 mL

8.2412 mL

16.4823 mL

5 mM

0.3296 mL

1.6482 mL

3.2965 mL

10 mM

0.1648 mL

0.8241 mL

1.6482 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Protocole

Cell Assay^[1]

Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Texas Red for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

For in vivo imaging, Texas Red (Sulforhodamine 101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Texas Red injection over the tail vein (10 mg/mL) has been reported to be successful^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (284 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Axel Nimmerjahn, et al. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods. 2004 Oct;1(1):31-7. [Content Brief]

[2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief]

[3]. Wang T, et al. Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. Neuropharmacology. 2022 Oct 1;217:109191. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.6482 mL	8.2412 mL	16.4823 mL	41.2058 mL
	5 mM	0.3296 mL	1.6482 mL	3.2965 mL	8.2412 mL
	10 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1206 mL
DMSO	15 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7471 mL
	20 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0603 mL
	25 mM	0.0659 mL	0.3296 mL	0.6593 mL	1.6482 mL
	30 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3735 mL
	40 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0301 mL
	50 mM	0.0330 mL	0.1648 mL	0.3296 mL	0.8241 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Texas Red

8 Cited Publications

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Texas Red

Publications Citing Use of MedChemExpress (MCE) Texas Red

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Protocole

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Texas Red Related Classifications

HY-101878 Related Classifications