Art. -Nr.: HY-101025

Nrf2-IN-1

11 Cited Publications

Kundenbewertung

Based on 11 publication(s) in Google Scholar

Nrf2-IN-1 is an inhibitor of nuclear factor-erythroid 2-related factor 2 (Nrf2). Nrf2-IN-1 is developed for the research of acute myeloid leukemia (AML).

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Reinheit: 99.88%
CAS. Nr.: 1610022-76-8
Formel: C₂₁H₂₂ClN₃O₂
Molecular Weight:383.87

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Nrf2-IN-1 Chemical Structure

CAS. Nr.: 1610022-76-8

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Nrf2-IN-1 is an inhibitor of nuclear factor-erythroid 2-related factor 2 (Nrf2). Nrf2-IN-1 is developed for the research of acute myeloid leukemia (AML)^[1].

IC₅₀ & Target

Nrf2^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.82 μM Compound: 4f	Cytotoxicity against human A549 cells assessed as growth inhibition after 24 to 48 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells assessed as growth inhibition after 24 to 48 hrs by sulforhodamine B assay	[PMID: 24996138]
THP-1	IC₅₀	5.33 μM Compound: 5	Cytotoxicity against human THP-1 cells incubated for 48 hrs by CCK8 assay Cytotoxicity against human THP-1 cells incubated for 48 hrs by CCK8 assay	[PMID: 39241669]

In Vitro

Nrf2-IN-1 (Compound 4f) (10 μM) inhibits Nrf2 activation^[1].
Nrf2-IN-1(1-20 μM; 48 hours) inhibits growth of the three AML cell^[1].
Nrf2-IN-1 (5-10 μM; 48 hours) induces apoptosis of three AML cells in vitro^[1].
Nrf2-IN-1 induces apoptotic signaling involving Bcl-2 and Bax^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

THP-1 cells, HL-60 cells, U937 cells
Concentration:

1 μM, 5 μM, 10 μM, 20 μM
Incubation Time:

48 hours
Result:

Inhibited growth of the three AML cell types at 5 μM, 10 μM or 20 μM for 48 hours.

Apoptosis Analysis^[1]

Cell Line:

THP-1 cells, HL-60 cells, U937 cells
Concentration:

5 μM, 10 μM
Incubation Time:

48 hours
Result:

Induced apoptosis in AML cells in vitro.

Western Blot Analysis^[1]

Cell Line:

THP-1 cells, HL-60 cells, U937 cells
Concentration:

5 μM, 10 μM
Incubation Time:

48 hours
Result:

Triggered caspase-dependent apoptotic signaling in AML cells.

In Vivo

Nrf2-IN-1 inhibits tumor growth via apoptosis in chicken eggs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1610022-76-8
Appearance Solid
Molecular Weight 383.87
Formel C₂₁H₂₂ClN₃O₂
Color White to off-white
SMILES

O=C(NO)C1=CC(C2=CC=C(Cl)C=C2)=NN1CC3=CC=C(C(C)(C)C)C=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (11)

All WB

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Journal Impact Factor

Int J Nanomedicine

Spatholobus suberectus Stem-Derived Extracellular Vesicle-Like Particles Attenuate Glucocorticoid-Induced Osteoporosis via NRF2/HO-1 Signaling. [Abstract]

2026 Apr 11:21:585928. PMID: 41993783
Clin Transl Med

ACOD1, rather than itaconate, facilitates p62-mediated activation of Nrf2 in microglia post spinal cord contusion. [Abstract]

2024 Apr;14(4):e1661. PMID: 38644791
Acupunct Herb Med

2024 Nov 12.
Clin Exp Hypertens

Glycyrrhetinic acid ameliorates chronic heart failure via the Nrf2 pathway. [Abstract]

2026 Dec 31;48(1):2607404. PMID: 41447318
Ecotoxicol Environ Saf

MC-LR triggers trophoblast ferroptosis at the maternal-fetal interface by directly targeting PKM2 to reprogram metabolism. [Abstract]

2025 Nov 15:307:119451. PMID: 41273830
Cancer Immunol Immunother

M2 tumor-associated macrophages resist to oxidative stress through heme oxygenase-1 in the colorectal cancer tumor microenvironment. [Abstract]

2023 Jul;72(7):2233-2244. PMID: 36869896

Nrf2-IN-1 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2023 Jul;72(7):2233-2244. [Abstract]

Nrf2-IN-1 (Nrf2 inhibitor; 5, 10, 20 μM; 48 h) decreases Nrf2 and HO-1 protein expressions in THP-1 cells in a dose dependent manner.
Inflammation

4-Octyl Itaconate Attenuates Postmenopausal Osteoporosis by Inhibiting Ferroptosis and Enhancing Osteogenesis via the Nrf2 Pathway. [Abstract]

2025 Feb 22. PMID: 39984770
Clin Exp Pharmacol Physiol

Exploring the Protective Role and Regulation of Prdx6 in Cisplatin-Induced AKI. [Abstract]

2026 Jan;53(1):e70093. PMID: 41267204
Arch Oral Biol

Taiwanese green propolis suppresses NLRP3 inflammasome activation and induces Nrf2/HO-1 pathway to reduce periodontal pathogen-induced endothelial inflammation. [Abstract]

2025 Oct:178:106364. PMID: 40753822
Cells Tissues Organs

July 08, 2021.
bioRxiv

ATF4 Coordinates Transcriptomic and Structural Adaptations in Aging Muscle. [Abstract]

2026 Mar 28:2026.03.27.711928. PMID: 41929138

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (651.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6050 mL

13.0252 mL

26.0505 mL

5 mM

0.5210 mL

2.6050 mL

5.2101 mL

10 mM

0.2605 mL

1.3025 mL

2.6050 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.17 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.17 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.17 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zhang J, et al. Discovery of a novel Nrf2 inhibitor that induces apoptosis of human acute myeloid leukemia cells. Oncotarget. 2017 Jan 31;8(5):7625-7636. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6050 mL	13.0252 mL	26.0505 mL	65.1262 mL
	5 mM	0.5210 mL	2.6050 mL	5.2101 mL	13.0252 mL
	10 mM	0.2605 mL	1.3025 mL	2.6050 mL	6.5126 mL
	15 mM	0.1737 mL	0.8683 mL	1.7367 mL	4.3417 mL
	20 mM	0.1303 mL	0.6513 mL	1.3025 mL	3.2563 mL
	25 mM	0.1042 mL	0.5210 mL	1.0420 mL	2.6050 mL
	30 mM	0.0868 mL	0.4342 mL	0.8683 mL	2.1709 mL
	40 mM	0.0651 mL	0.3256 mL	0.6513 mL	1.6282 mL
	50 mM	0.0521 mL	0.2605 mL	0.5210 mL	1.3025 mL
	60 mM	0.0434 mL	0.2171 mL	0.4342 mL	1.0854 mL
	80 mM	0.0326 mL	0.1628 mL	0.3256 mL	0.8141 mL
	100 mM	0.0261 mL	0.1303 mL	0.2605 mL	0.6513 mL

Keywords

Nrf2-IN-1

11 Cited Publications

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Nrf2-IN-1

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Anti-Colorectal Cancer Compound Library

Heterocyclic Compound Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Nrf2-IN-1

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Nrf2-IN-1

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Anti-Colorectal Cancer Compound Library

Heterocyclic Compound Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Nrf2-IN-1

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Nrf2-IN-1 Related Classifications

HY-101025 Related Classifications