Art. -Nr.: HY-122705

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RAD51-IN-1

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RAD51-IN-1, a derivative of B02, is a potent inhibitor of RAD51. RAD51-IN-1 can be used for cancer research.

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Reinheit: 99.18%
CAS. Nr.: 2101739-18-6
Formel: C₂₂H₁₆ClN₃O
Molecular Weight:373.83

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

RAD51-IN-1 Chemical Structure

CAS. Nr.: 2101739-18-6

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

RAD51-IN-1, a derivative of B02, is a potent inhibitor of RAD51. RAD51-IN-1 can be used for cancer research^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BT-549	IC₅₀	6.4 μM Compound: 17	Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
HCC1937	IC₅₀	13.7 μM Compound: 17	Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
Hs-578T	IC₅₀	4.2 μM Compound: 17	Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MCF-10A	IC₅₀	48.3 μM Compound: 17	Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-231	IC₅₀	6.7 μM Compound: 17	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-436	IC₅₀	4.1 μM Compound: 17	Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
MDA-MB-468	IC₅₀	5.7 μM Compound: 17	Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]
SUM-159-PT	IC₅₀	5.5 μM Compound: 17	Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay	[PMID: 28545975]

In Vitro

RAD51 is a vital component of the homologous recombination DNA repair pathway and is overexpressed in drug-resistant cancers, including aggressive triple-negative breast cancer (TNBC)^[1].
RAD51-IN-1 (10 μM) decreases the ratio of RAD51 positive cells/cH2AX positive cells in MDA-MB-231 cell exposure to 6 Gy irradiation^[1].
RAD51-IN-1 (10 μM) significantly inhibits DNA damage induced RAD51 foci formation with 6 Gy irradiation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 2101739-18-6
Appearance Solid
Molecular Weight 373.83
Formel C₂₂H₁₆ClN₃O
Color Yellow to orange
SMILES

O=C1N(CC2=CC=C(Cl)C=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 62.5 mg/mL (167.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6750 mL

13.3751 mL

26.7501 mL

5 mM

0.5350 mL

2.6750 mL

5.3500 mL

10 mM

0.2675 mL

1.3375 mL

2.6750 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ambber Ward, et al. Quinazolinone derivatives as inhibitors of homologous recombinase RAD51. Bioorg Med Chem Lett. 2017 Jul 15;27(14):3096-3100. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6750 mL	13.3751 mL	26.7501 mL	66.8753 mL
	5 mM	0.5350 mL	2.6750 mL	5.3500 mL	13.3751 mL
	10 mM	0.2675 mL	1.3375 mL	2.6750 mL	6.6875 mL
	15 mM	0.1783 mL	0.8917 mL	1.7833 mL	4.4584 mL
	20 mM	0.1338 mL	0.6688 mL	1.3375 mL	3.3438 mL
	25 mM	0.1070 mL	0.5350 mL	1.0700 mL	2.6750 mL
	30 mM	0.0892 mL	0.4458 mL	0.8917 mL	2.2292 mL
	40 mM	0.0669 mL	0.3344 mL	0.6688 mL	1.6719 mL
	50 mM	0.0535 mL	0.2675 mL	0.5350 mL	1.3375 mL
	60 mM	0.0446 mL	0.2229 mL	0.4458 mL	1.1146 mL
	80 mM	0.0334 mL	0.1672 mL	0.3344 mL	0.8359 mL
	100 mM	0.0268 mL	0.1338 mL	0.2675 mL	0.6688 mL

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RAD51-IN-1

Anti-Breast Cancer Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RAD51-IN-1

Anti-Breast Cancer Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RAD51-IN-1 Related Classifications

HY-122705 Related Classifications