Soyasapogenol A
Based on 1 Customer Validation
Soyasapogenol A is a triterpenoid aglycone of soyasaponins. Soyasapogenol A has activities such as anti-inflammation, anti-cancer, hepatoprotection and anti-HSV-1. Soyasapogenol A can be used in the research of tumors and immune inflammatory diseases.
For research use only. We do not sell to patients.
- Purity: 99.28%
- CAS No.: 508-01-0
- Formula: C30H50O4
- Molecular Weight:474.72
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
HY-L002
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Natural Product Library
HY-L021
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Anti-Cancer Compound Library
HY-L025
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Antiviral Compound Library
HY-L027
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Medicine Food Homology Compound Library
HY-L055
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Terpenoids Library
HY-L056
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Traditional Chinese Medicine Active Compound Library
HY-L065
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Food-Sourced Compound Library
HY-L094
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Anti-Cancer Natural Product Library
HY-L107
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Antiviral Traditional Chinese Medicine Active Compound Library
HY-L113
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Anti-inflammatory Traditional Chinese Medicine Active Compound Library
HY-L114
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Plant-Sourced Natural Product Library
HY-L115
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Human Metabolite Library
HY-L123
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Natural Product Library Plus
HY-L021P
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Natural Product and Natural Product-Like Compound Library
HY-L021M
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Bioactive Compound Library
HY-L001
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Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library
HY-L194
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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High-Throughput Natural Product Library
HY-L206
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Diarrhea-Related Traditional Chinese Medicine Active Compound Library
HY-L224
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Mongolian Medicine Compound Library
HY-L238
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
>100 μM
Compound: 2
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Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of HMGB1-induced NO production pretreated with compounds for 15 mins followed by HMGB1 stimulation measured after 24 hrs by Griess reagent based assay
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of HMGB1-induced NO production pretreated with compounds for 15 mins followed by HMGB1 stimulation measured after 24 hrs by Griess reagent based assay
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[PMID: 34800877] |
Soyasapogenol A (0-0.5 mg/mL; in the form of concentrated extract; 24-72 h) can inhibit the proliferation of Hep-G2 cells (LC50: 0.052 mg/mL) and induce cell cycle arrest and apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice (21-25 g) treated Concanavalin A (HY-P2149)[2]
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Dosage:2 mg
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Administration:Subcutaneous injection (s.c.); at 2 and 14 h prior to an intravenous injection of Concanavalin A
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Result:Significantly lowered the elevated plasma alanine aminotransferase activity.
Decreased the number of apoptotic bodies in the liver parenchymal cells.
Reduced the number of infiltrating inflammatory cells in the liver.
Significantly lowered the elevated level of plasma tumor necrosis factor-α and interferon-γ.
Chemical Information
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CAS No. 508-01-0
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Appearance Solid
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Molecular Weight 474.72
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Formula C30H50O4
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Color White to off-white
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SMILES
C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H]([C@H](O)C(C)(C)C4)O)CC3)=CC[C@]1([H])[C@@]5([C@@]([C@](C)([C@@H](O)CC5)CO)([H])CC2)C)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (210.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.27 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang W, et al. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem. 2008 Apr 23;56(8):2603-8. [Content Brief]
[2]. Kuzuhara H, et al. Protective effects of soyasapogenol A on liver injury mediated by immune response in a concanavalin A-induced hepatitis model. Eur J Pharmacol. 2000 Mar 10;391(1-2):175-81. [Content Brief]
[3]. Guang C, et al. Biological functionality of soyasaponins and soyasapogenols. J Agric Food Chem. 2014 Aug 20;62(33):8247-55. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1065 mL | 10.5325 mL | 21.0650 mL | 52.6626 mL |
| 5 mM | 0.4213 mL | 2.1065 mL | 4.2130 mL | 10.5325 mL | |
| 10 mM | 0.2107 mL | 1.0533 mL | 2.1065 mL | 5.2663 mL | |
| 15 mM | 0.1404 mL | 0.7022 mL | 1.4043 mL | 3.5108 mL | |
| 20 mM | 0.1053 mL | 0.5266 mL | 1.0533 mL | 2.6331 mL | |
| 25 mM | 0.0843 mL | 0.4213 mL | 0.8426 mL | 2.1065 mL | |
| 30 mM | 0.0702 mL | 0.3511 mL | 0.7022 mL | 1.7554 mL | |
| 40 mM | 0.0527 mL | 0.2633 mL | 0.5266 mL | 1.3166 mL | |
| 50 mM | 0.0421 mL | 0.2107 mL | 0.4213 mL | 1.0533 mL | |
| 60 mM | 0.0351 mL | 0.1755 mL | 0.3511 mL | 0.8777 mL | |
| 80 mM | 0.0263 mL | 0.1317 mL | 0.2633 mL | 0.6583 mL | |
| 100 mM | 0.0211 mL | 0.1053 mL | 0.2107 mL | 0.5266 mL |
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