Pembrolizumab

Human IgG4 kappa

Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Pembrolizumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).

Loaded Pembrolizumab on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His, HY-P7396) with an affinity constant of 1.898E-09 M as determined in BLI assay.

Immobilized PD-1 Protein, Human can bind Pembrolizumab. The EC₅₀ for this effect is 1.8 ng/mL.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]

Customer Validation
Plots of tumor growth and tumor weight for each group (5 mice/group). The results showed that in mice, the combination of Pembrolizumab and Evolocumab significantly enhanced anti-tumor efficacy compared to Pembrolizumab alone (100 µg/mouse; i.p., every 7 days).

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jan 21;16(1):34. [Abstract]

Customer Validation
Pembrolizumab (pembro) (5-10 µg/mL, 24 h). AMM16 cells were transfected with control siRNA (siRNA ctrl; left panels) or AR siRNA (siRNA AR; right panels) and treated with the indicated ICIs at 35 μg/ml. The red fluorescence from the propidium iodide (PI) and the green fluorescence from the acridin orange (AO) staining indicate dead and total cells, respectively.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 16;31(1):278. [Abstract]

Customer Validation
Scratch assay comparing the migration ability of MDA-MB-231/PEM and MDA-MB-231 cells treated with 10 µg/mL Pembrolizumab (PEM).

Pembrolizumab purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Aug 6;12(8):e009024. [Abstract]

Customer Validation
The PD-1 expression of macrophages isolated from the subcutaneous tumor microenvironment of mice was detected by flow cytometry (n=5). The results showed that Pembrolizumab (anti-PD-1 Ab) (20 µg/mL, 24 h) reduced PD-1 expression.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jul 12;13(1):4032. [Abstract]

Customer Validation
PD-L1/PD-1 binding activity assay after treatment with free aPD-L1 (Pembrolizumab) or SPIND2 (40 µg/mL) with or without US irradiation.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jul 12;13(1):4032. [Abstract]

Customer Validation
Relative tumor volumes of primary tumors of Panc02 tumor-bearing C57BL/6 mice (n = 6) after systemic injection of saline, free-drug mixture (on day 0, 3, and 6, 4 mg/kg body weight for NLG919 and aPD-L1(Pembrolizumab)), or SPIND2 (0.2 mL, 0.6 mg/mL) with or without US irradiation.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 May 9;12(5):465. [Abstract]

Customer Validation
4T1 tumor-bearing mice treated with anti-PD-1 mAbs (Pembrolizumab, 10 mg/kg; i.p.; twice a week) or LY2940002 (25 mg/kg; i.p.; twice a week) were euthanized at Day21 and the tumor volume were measured. The results showed that the administration of​ anti-PD-1 mAbs (Pembrolizumab), LY294002, or their combination​ significantly inhibited tumor growth in tumor-bearing mice.

Pembrolizumab purchased from MedChemExpress. Usage Cited in: ACS Appl Bio Mater. 2020 Oct 19;3(10):7080-7086. [Abstract]

Customer Validation
The immunofluorescence images of the tumor from the PBS- (A), random sequence- (B), anti-PD-1 antibody- (Pembrolizumab (5 mg/kg; s.c.; every other day for 16 days)) (C) and isotype antibody-treated group (D); The scale bar in the images is 50 µm.