2. Others Neuronal Signaling
  3. Environmental Pollutants Cholinesterase (ChE)
  4. Chlorpyrifos

Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons.

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CAS No. : 2921-88-2

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Based on 2 publication(s) in Google Scholar

Other Forms of Chlorpyrifos:

Chlorpyrifos Related Antibodies

Top Publications Citing Use of Products
In Vivo Efficacy Study

    Chlorpyrifos purchased from MedChemExpress. Usage Cited in: Chemosphere. 2022 Apr:293:133522.  [Abstract]

    Exposure to carbendazim and chlorpyrifos (0-2.35 mg/mL) together at the indicated concentrations generally reduced swimming activity. Although angular velocity, turn angle, and meander did not differ significantly from controls during the 3-min free-swimming period under darkness, average velocity, total distance traveled, and light-dark acceleration were significantly reduced at higher combined doses (all p < 0.05).

    Chlorpyrifos purchased from MedChemExpress. Usage Cited in: Chemosphere. 2022 Apr:293:133522.  [Abstract]

    Effects of chlorpyrifos (0-2.52 mg/L, 120 h) on larval zebrafish swimming behavior at 5 dpf.

    Chlorpyrifos purchased from MedChemExpress. Usage Cited in: Chemosphere. 2022 Apr:293:133522.  [Abstract]

    Combination index (CI) values of carbendazim and chlorpyrifos on zebrafish average swim velocity and acceleration during the lightdark transition.

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    • Biological Activity

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    Description

    Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons[1][2][3][4].

    IC50 & Target

    AChE

     

    In Vitro

    Chlorpyrifos mediates desulfuration to produce chlorpyrifos oxon (CPO), which has higher affinity toward the active site of serine-dependent ester hydrolases such as AChE[2].
    Chlorpyrifos (3.9-250 μM; 24-72 h) is toxic to oligodendrocyte progenitors[3].
    Chlorpyrifos toxicity is associated with (7.5-480 μM; 18 h) nuclear condensation and elevation of caspase 3/7 activity, (60 μM; 2, 4 h) Heme oxygenase-1 mRNA expression in Central Glia (CG-4) cells, and (30, 60, 120 μM; 24 h) enhances H2DCF-DA intensity[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Chlorpyrifos Related Antibodies

    Cell Viability Assay[3]

    Cell Line: Oligodendrocyte CG-4 cells
    Concentration: 3.9, 7.8, 15.6, 31.25, 62.5, 125, and 250 μM
    Incubation Time: 24, 48, and 72 hours
    Result: Significantly inhibited cell viability over 62.5 μM.

    Immunofluorescence[3]

    Cell Line: Oligodendrocyte CG-4 cells
    Concentration: 0, 30, 60, 120 μM
    Incubation Time: 24 hours
    Result: Resulted nuclear condensation and elevation in a dose-dependent manner.
    In Vivo

    Chlorpyrifos (97-276 mg/kg; p.o.; single dose) has moderately acute oral toxicity, with lethal dose, 50% (LD50) of 97-276 mg/kg in rats[2].
    Chlorpyrifos (1 mg/kg and 5 mg/kg for 1 mL/kg; s.c.; once daily for 3 d) has adverse effect on learning and memory abilities of rats, and affects pregnant rats on gestational days 9-12, results offspring behavioral abnormalities[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    350.59

    Formula

    C9H11Cl3NO3PS
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    S=P(OCC)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (285.23 mM; Need ultrasonic)

    DMSO : 50 mg/mL (142.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8523 mL 14.2617 mL 28.5233 mL
    5 mM 0.5705 mL 2.8523 mL 5.7047 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (7.13 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.94%

    SDS (623 KB)

    COA (252 KB) HNMR (246 KB) CNMR (256 KB) LCMS (89 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.8523 mL 14.2617 mL 28.5233 mL 71.3084 mL
    5 mM 0.5705 mL 2.8523 mL 5.7047 mL 14.2617 mL
    10 mM 0.2852 mL 1.4262 mL 2.8523 mL 7.1308 mL
    15 mM 0.1902 mL 0.9508 mL 1.9016 mL 4.7539 mL
    20 mM 0.1426 mL 0.7131 mL 1.4262 mL 3.5654 mL
    25 mM 0.1141 mL 0.5705 mL 1.1409 mL 2.8523 mL
    30 mM 0.0951 mL 0.4754 mL 0.9508 mL 2.3769 mL
    40 mM 0.0713 mL 0.3565 mL 0.7131 mL 1.7827 mL
    50 mM 0.0570 mL 0.2852 mL 0.5705 mL 1.4262 mL
    60 mM 0.0475 mL 0.2377 mL 0.4754 mL 1.1885 mL
    80 mM 0.0357 mL 0.1783 mL 0.3565 mL 0.8914 mL
    100 mM 0.0285 mL 0.1426 mL 0.2852 mL 0.7131 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Chlorpyrifos Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Chlorpyrifos2921-88-2Environmental PollutantsCholinesterase (ChE)Environmental ContaminantsInhibitorinhibitorinhibit

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