2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Chlorpyrifos

Chlorpyrifos 

Cat. No.: HY-B0815 Purity: 99.94%
COA Handling Instructions

Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons.

For research use only. We do not sell to patients.

Chlorpyrifos Chemical Structure

Chlorpyrifos Chemical Structure

CAS No. : 2921-88-2

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50 mg USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 75 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons[1][2][3][4].

IC50 & Target

AChE

 

In Vitro

Chlorpyrifos mediates desulfuration to produce chlorpyrifos oxon (CPO), which has higher affinity toward the active site of serine-dependent ester hydrolases such as AChE[2].
Chlorpyrifos (3.9-250 μM; 24-72 h) is toxic to oligodendrocyte progenitors[3].
Chlorpyrifos toxicity is associated with (7.5-480 μM; 18 h) nuclear condensation and elevation of caspase 3/7 activity, (60 μM; 2, 4 h) Heme oxygenase-1 mRNA expression in Central Glia (CG-4) cells, and (30, 60, 120 μM; 24 h) enhances H2DCF-DA intensity[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: Oligodendrocyte CG-4 cells
Concentration: 3.9, 7.8, 15.6, 31.25, 62.5, 125, and 250 μM
Incubation Time: 24, 48, and 72 hours
Result: Significantly inhibited cell viability over 62.5 μM.

Immunofluorescence[3]

Cell Line: Oligodendrocyte CG-4 cells
Concentration: 0, 30, 60, 120 μM
Incubation Time: 24 hours
Result: Resulted nuclear condensation and elevation in a dose-dependent manner.
In Vivo

Chlorpyrifos (97-276 mg/kg; p.o.; single dose) has moderately acute oral toxicity, with lethal dose, 50% (LD50) of 97-276 mg/kg in rats[2].
Chlorpyrifos (1 mg/kg and 5 mg/kg for 1 mL/kg; s.c.; once daily for 3 d) has adverse effect on learning and memory abilities of rats, and affects pregnant rats on gestational days 9-12, results offspring behavioral abnormalities[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

350.59

Appearance

Solid

Formula

C9H11Cl3NO3PS
CAS No.
SMILES

S=P(OCC)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (285.23 mM; Need ultrasonic)

DMSO : 50 mg/mL (142.62 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8523 mL 14.2617 mL 28.5233 mL
5 mM 0.5705 mL 2.8523 mL 5.7047 mL
10 mM 0.2852 mL 1.4262 mL 2.8523 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 6.25 mg/mL (17.83 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (7.13 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.94%

COA (252 KB) HNMR (246 KB) LCMS (262 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Chlorpyrifos2921-88-2Cholinesterase (ChE)Inhibitorinhibitorinhibit

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