1. Membrane Transporter/Ion Channel
  2. Chloride Channel
    Potassium Channel
  3. DCPIB

DCPIB 

Cat. No.: HY-103371 Purity: 99.93%
Handling Instructions

DCPIB est un inhibiteur des canaux anioniques à volume régulé (VRAC) qui est sélectif, réversible et puissant. DCPIB active en fonction de la tension les canaux potassiques TREK1 et TRAAK et inhibe TRESK, TASK1 et TASK3 (IC50s de 0,14, 0,95, 50,72 μM, respectivement). DCPIB est également un bloqueur sélectif du courant de chlorure induit par le gonflement (ICl, gonflement), avec un IC50 de 4,1 μM dans les cellules CPAE.

DCPIB is a selective, reversible and potent inhibitor of volume-regulated anion channels (VRAC). DCPIB voltage-dependently activates potassium channels TREK1 and TRAAK, and inhibits TRESK, TASK1 and TASK3 (IC50s: 0.14, 0.95, 50.72 μM, respectively). DCPIB is also a selective blocker of swelling-induced chloride current (ICl,swell), with an IC50 of 4.1 μM. DCPIB is a useful tool for investigating structure-function studies of K2P channels.

For research use only. We do not sell to patients.

DCPIB Chemical Structure

DCPIB Chemical Structure

CAS No. : 82749-70-0

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Solution
10 mM * 1 mL in DMSO USD 109 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
USD 109 In-stock
Estimated Time of Arrival: December 31
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5 mg USD 86 In-stock
Estimated Time of Arrival: December 31
10 mg USD 143 In-stock
Estimated Time of Arrival: December 31
50 mg USD 605 In-stock
Estimated Time of Arrival: December 31
100 mg USD 768 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

DCPIB is a selective, reversible and potent inhibitor of volume-regulated anion channels (VRAC). DCPIB voltage-dependently activates potassium channels TREK1 and TRAAK, and inhibits TRESK, TASK1 and TASK3 (IC50s: 0.14, 0.95, 50.72 μM, respectively). DCPIB is also a selective blocker of swelling-induced chloride current (ICl,swell), with an IC50 of 4.1 μM. DCPIB is a useful tool for investigating structure-function studies of K2P channels[1][2].

IC50 & Target

IC50:0.14 μM (TRESK), 0.95 μM (TASK1), 50.72 μM (TASK3)[1], 4.1 μM (ICl,swell, CPAE cells)[2]

In Vitro

DCPIB (10 μM) activates TREK1 and enhances TRAAK currents in COS-7 cells[1].
DCPIB (10 μM) prominently and reversibly suppresses TRESK currents in COS-7 cells, with an IC50 of 0.14 μM[1].
DCPIB (10 μM) displays selectivity for ICl,swell and has no significant inhibitory effects on ICl,Ca in CPAE cells[2].
DCPIB (10 μM, 5 min) has no effect on attenuate subsequent swelling in cardiomyocytes[2].
DCPIB (10 μM, 3 h) inhibits LPS-induced MAPK activation in BV2 cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence[3]

Cell Line: BV2 cells
Concentration: 10 μM
Incubation Time: 3 h
Result: Significantly decreased Ki67 positive staining microglia and pro-inflammatory cytokine (TNF-α, IL-1β) secretion.

Western Blot Analysis[3]

Cell Line: BV2 cells
Concentration: 10 μM
Incubation Time: 3 h
Result: Inhibited migratory potential of transient OGD (Oxygen-glucose deprivation) exposed BV2 cells.
In Vivo

LDN-212854 (intracerebroventricular infusion, 1 mM, 10 μL) suppresses microglial activation and ameliorates neuronal damage in rMCAO rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Reversible middle cerebral artery occlusion (rMCAO) model[3]
Dosage: 1 mM, 10 μL
Administration: Administered manually for 20s by intracerebroventricular infusion
Result: Diminished Pyramidal neurons injury induced by rMCAO in the CA1 region.
Molecular Weight

427.36

Formula

C22H28Cl2O4

CAS No.
SMILES

O=C(O)CCCOC1=CC2=C(C(C(C3CCCC3)(CCCC)C2)=O)C(Cl)=C1Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (233.99 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3399 mL 11.6997 mL 23.3995 mL
5 mM 0.4680 mL 2.3399 mL 4.6799 mL
10 mM 0.2340 mL 1.1700 mL 2.3399 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (4.87 mM); Clear solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.93%

References
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DCPIB
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