2. Autophagy Apoptosis Protein Tyrosine Kinase/RTK
  3. Autophagy Ferroptosis ROS Kinase
  4. DHODH-IN-33

DHODH-IN-33 is a selective dihydroorotate dehydrogenase (DHODH) inhibitor with potent activity against A549 (IC50 = 5.22 μM) and 5637 (IC50 = 3.03 μM). DHODH-IN-33 induces autophagy-dependent ferroptosis (mitochondrial dysfunction, lipid peroxidation, and ROS accumulation) with no notable toxicity in vivo. DHODH-IN-33 exerts anti-cancer effect by promoting the autophagy-dependent degradation of DHODH. DHODH-IN-33 can be used for non-small cell lung cancer and bladder cancer.

For research use only. We do not sell to patients.

DHODH-IN-33

DHODH-IN-33 Chemical Structure

CAS No. : 3026839-88-0

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

DHODH-IN-33 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DHODH-IN-33 is a selective dihydroorotate dehydrogenase (DHODH) inhibitor with potent activity against A549 (IC50 = 5.22 μM) and 5637 (IC50 = 3.03 μM). DHODH-IN-33 induces autophagy-dependent ferroptosis (mitochondrial dysfunction, lipid peroxidation, and ROS accumulation) with no notable toxicity in vivo. DHODH-IN-33 exerts anti-cancer effect by promoting the autophagy-dependent degradation of DHODH. DHODH-IN-33 can be used for non-small cell lung cancer and bladder cancer[1].

In Vitro

DHODH-IN-33 (compound 3af) (48 h) exhibits potent activity against A549 (IC50 = 5.22 μM) and 5637 (IC50 = 3.03 μM)[1].
DHODH-IN-33 (5 and 10 μM, 48 h) does not induce A549 death via the apoptosis mechanism[1].
DHODH-IN-33 (5 and 10 μM, 24 h) links the induction of ferroptosis to the activation of autophagy in A549 cells, primarily through the coordinated degradation of redox-stabilizing enzymes[1].
DHODH-IN-33 (5 and 10 μM, 48 h) induces autophagy-dependent ferroptosis as the primary death pathway in A549 cells by initiating autophagy, inhibiting DHODH and lipid peroxidation, which together act as upstream switches to drive the downstream execution event of lipid peroxidation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DHODH-IN-33 Related Antibodies

Apoptosis Analysis[1]

Cell Line: A549 cells
Concentration: 5, 10 μM
Incubation Time: 48 h
Result: Exhibited 10.95% and 21.24% apoptosis at concentration of 5 μM and 10 μM, respectively.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 5, 10 μM
Incubation Time: 24 h
Result: Downregulated the expression of DHODH, but had little effect on the level of GPX4.
Increased the Beclin-1 and LC3 II (lipidated form) levels.

Cell Viability Assay[1]

Cell Line: A549 cells
Concentration: 5, 10 μM
Incubation Time: 48 h
Result: Inhibited the cell viability of the A549 cells at various concentrations, particularly at a concentration of 10 μM.
Caused cytotoxicity can be reversed by 3-MA, Lip-1 and MitoQH2 with efficacy in the order of 3-MA > Lip-1 > MitoQH2.

Western Blot Analysis[1]

Cell Line: A549 cells
Concentration: 5, 10 μM
Incubation Time: 24 h
Result: Caused an upregulation of LC3 II and a downregulation of DHODH that were not significantly reversed by the addition of either the lipid peroxidation inhibitor Lip-1 or the DHODH enzyme substitute MitoQH2.
In Vivo

DHODH-IN-33 (compound 3af) (10 and 20 mg/kg, i.p., once every 48 h for 14 days) exerts anti-tumor effect via an autophagy-dependent ferroptosis pathway in murine subcutaneous A549 xenograft model, in which ferroptosis was the primary mechanism and autophagy activation a crucial prerequisite, all while maintaining a favorable tolerability profile in BALB/c nude mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice (8 week-old)[1]
Dosage: 10 or 20 mg/kg
Administration: i.p., once every 48 h for 14 days
Result: Achieved tumor growth inhibition rates of 19.37% and 58.33% on day 14 at the corresponding doses of 10 mg/kg and 20 mg/kg, respectively.
Upregulated the expression level of LC3 II at administration of 20 mg/kg.
Increased ACSL4 protein.
Molecular Weight

407.48

Formula

C23H21NO4S
CAS No.
SMILES

COC1=CC2=C(N(C3C4=CC=CC=C4OC3C2)S(=O)(C5=CC=C(C=C5)C)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

DHODH-IN-33 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DHODH-IN-333026839-88-0AutophagyFerroptosisROS KinaseDHODHferroptosisdihydroorotate dehydrogenaselung cancerInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DHODH-IN-33
Cat. No.:
HY-179531
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.