2. Metabolic Enzyme/Protease
  3. Dipeptidyl Peptidase
  4. Diprotin A

Diprotin A  (Synonyms: Ile-Pro-Ile)

Cat. No.: HY-111174 Purity: 99.58%
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Diprotin A (Ile-Pro-Ile) is an inhibitor of dipeptidyl peptidase IV (DPP-IV).

For research use only. We do not sell to patients.

Diprotin A Chemical Structure

Diprotin A Chemical Structure

CAS No. : 90614-48-5

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Based on 1 publication(s) in Google Scholar

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Diprotin A Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Diprotin A (Ile-Pro-Ile) is an inhibitor of dipeptidyl peptidase IV (DPP-IV)[1].

IC50 & Target

IC50: DPP-IV[1]
In Vitro

Diprotin A (100 μM; 30 minutes after CXCR4-blocker or Src-inhibitor treatment) induces the phosphorylation of Src [Tyr 416] and VE-cadherin [Tyr731] in hECs in both normoxia and H/R conditions in human endothelial cells and disrupts endothelial cell-to-cell junctions, which are attenuated by CXCR4 (receptor of SDF-1α)-blocker or Src-inhibitor[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Diprotin A Related Antibodies

Western Blot Analysis[1]

Cell Line: Human endothelial cells[1]
Concentration: 100 μM
Incubation Time: 30 minutes after CXCR4-blocker or Src-inhibitor treatment
Result: Induced the phosphorylation of Src [Tyr 416] and VE-cadherin [Tyr731] in hECs.
In Vivo

Diprotin A (intraperitoneal injection; 70 μg/kg; twice daily; 7 days) increases the phosphorylation of Src and VE-cadherin and aggravates vascular leakage in the retinas. Collectively, Diprotin A induces vascular leakage by augmenting the SDF-1α/CXCR4/Src/VE-cadherin signaling pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Streptozotocin-induced diabetic retinopathy model in wild-type C57/BL6 mice[1]
Dosage: 70 μg/kg
Administration: Intraperitoneal injection; twice daily; 7 days
Result: Induced vascular leakage by augmenting the SDF-1α/CXCR4/Src/VE-cadherin signaling pathway.
Molecular Weight

341.45

Formula

C17H31N3O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC[C@H](C)[C@@H](C(O)=O)NC([C@H]1N(C([C@H]([C@@H](C)CC)N)=O)CCC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (292.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (292.87 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9287 mL 14.6434 mL 29.2869 mL
5 mM 0.5857 mL 2.9287 mL 5.8574 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.9287 mL 14.6434 mL 29.2869 mL 73.2172 mL
5 mM 0.5857 mL 2.9287 mL 5.8574 mL 14.6434 mL
10 mM 0.2929 mL 1.4643 mL 2.9287 mL 7.3217 mL
15 mM 0.1952 mL 0.9762 mL 1.9525 mL 4.8811 mL
20 mM 0.1464 mL 0.7322 mL 1.4643 mL 3.6609 mL
25 mM 0.1171 mL 0.5857 mL 1.1715 mL 2.9287 mL
30 mM 0.0976 mL 0.4881 mL 0.9762 mL 2.4406 mL
40 mM 0.0732 mL 0.3661 mL 0.7322 mL 1.8304 mL
50 mM 0.0586 mL 0.2929 mL 0.5857 mL 1.4643 mL
60 mM 0.0488 mL 0.2441 mL 0.4881 mL 1.2203 mL
80 mM 0.0366 mL 0.1830 mL 0.3661 mL 0.9152 mL
100 mM 0.0293 mL 0.1464 mL 0.2929 mL 0.7322 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Diprotin A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diprotin A90614-48-5Ile-Pro-IleDipeptidyl PeptidaseDPPDiabetic retinopathynormoxiaH/R conditionendothelial cellvascular leakagehECsInhibitorinhibitorinhibit

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