Cat. No.: HY-P1334

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DPDPE

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

DPDPE, an opioid peptide, is a selective δ-opioid receptor?(DOR) agonist?with anticonvulsant effects.

For research use only. We do not sell to patients.

Purity: 99.21%
CAS No.: 88373-73-3
Formula: C₃₀H₃₉N₅O₇S₂
Molecular Weight:645.79

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

DPDPE Chemical Structure

CAS No.: 88373-73-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

DPDPE, an opioid peptide, is a selective δ-opioid receptor?(DOR) agonist?with anticonvulsant effects^[1].

IC₅₀ & Target

δ Opioid Receptor/DOR

All Opioid Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	>10000 nM Compound: DPDPE	Stimulation of [35S]GTP-gamma-S, binding to recombinant human Opioid receptor mu 1 expressed in CHO cells Stimulation of [35S]GTP-gamma-S, binding to recombinant human Opioid receptor mu 1 expressed in CHO cells	[PMID: 12672258]
CHO	EC₅₀	>10000 nM Compound: DPDPE	Stimulation of U-69,593 binding at human recombinant Opioid receptor kappa 1 transfected into CHO cells. Stimulation of U-69,593 binding at human recombinant Opioid receptor kappa 1 transfected into CHO cells.	[PMID: 12672258]
CHO	EC₅₀	1.3 nM Compound: DPDPE	Stimulation of [35S]GTP-gamma-S, binding at human recombinant Opioid receptor delta 1 transfected into CHO cells. Stimulation of [35S]GTP-gamma-S, binding at human recombinant Opioid receptor delta 1 transfected into CHO cells.	[PMID: 12672258]
CHO	EC₅₀	1.6 nM Compound: DPDPE	Inhibition of [35S]GTP-gamma-S binding to human delta opioid receptor expressed in CHO cells Inhibition of [35S]GTP-gamma-S binding to human delta opioid receptor expressed in CHO cells	[PMID: 16509592]
CHO	EC₅₀	16 nM Compound: DPDPE	Agonist activity at human delta opioid receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human delta opioid receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 18637671]
CHO	EC₅₀	1.6 nM Compound: DPDPE, c[D-Pen2, D-Pen5]enkephalin	Agonist activity at human delta opioid receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human delta opioid receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 21128594]
CHO	EC₅₀	2.05 nM Compound: DPDPE	Agonist activity at mouse cloned delta opioid receptor expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at mouse cloned delta opioid receptor expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 24103580]
CHO	EC₅₀	9.29 nM Compound: DPDPE	Agonist activity at human DOR expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human DOR expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 25248680]
CHO	EC₅₀	0.8 nM Compound: DPDPE	Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells assessed as change in cell morphology measured over 30 mins by label free binding assay Agonist activity at delta opioid receptor (unknown origin) expressed in CHO cells assessed as change in cell morphology measured over 30 mins by label free binding assay	[PMID: 25559207]
CHO	EC₅₀	8.01 nM Compound: DPDPE	Inverse agonist activity at human DOR expressed in CHO cell membranes by [35S]GTPgammaS binding assay Inverse agonist activity at human DOR expressed in CHO cell membranes by [35S]GTPgammaS binding assay	[PMID: 26048798]
CHO	EC₅₀	13.2 nM Compound: DPDPE	Agonist activity at human delta opioid receptor over-expressed in CHO cell membrane after 1 hr by [35S]GTPgammaS binding assay Agonist activity at human delta opioid receptor over-expressed in CHO cell membrane after 1 hr by [35S]GTPgammaS binding assay	[PMID: 26525865]
CHO	IC₅₀	1.3 nM Compound: DPDPE	Displacement of [3H]DADLE from human recombinant delta-type 2 opioid receptor expressed in CHO cells Displacement of [3H]DADLE from human recombinant delta-type 2 opioid receptor expressed in CHO cells	[PMID: 26988801]
CHO	EC₅₀	0.12 nM Compound: DPDPE	Agonist activity at human DOR expressed in CHO cell membranes after 60 mins by [35S]GTP-gamma-S binding assay Agonist activity at human DOR expressed in CHO cell membranes after 60 mins by [35S]GTP-gamma-S binding assay	[PMID: 27096047]
CHO	EC₅₀	10 nM Compound: DPDPE	Agonist activity at mouse delta opioid receptor-1 expressed in CHO cell membranes assessed as [35S]GTPgammaS binding incubated for 60 mins by scintillation spectroscopic analysis Agonist activity at mouse delta opioid receptor-1 expressed in CHO cell membranes assessed as [35S]GTPgammaS binding incubated for 60 mins by scintillation spectroscopic analysis	[PMID: 27556704]
CHO	IC₅₀	1.3 nM Compound: DPDPE	Displacement of [3H]DADLE from human recombinant delta opioid receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]DADLE from human recombinant delta opioid receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	1.3 x 10^-9 M Compound: DPDPE	Displacement of [3H]DADLE from human recombinant delta opioid receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [3H]DADLE from human recombinant delta opioid receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
CHO	IC₅₀	0.63 nM Compound: DPDPE	Binding affinity against rat Opioid receptor delta 1 expressed in CHO cells determined by competitive inhibition of 1 nM [3H]DPDPE. Binding affinity against rat Opioid receptor delta 1 expressed in CHO cells determined by competitive inhibition of 1 nM [3H]DPDPE.	[PMID: 9873480]
CHO	IC₅₀	4 nM Compound: DPDPE	Inhibitory activity against forskolin-stimulated cAMP production in chinese hamster ovary cells expressing Opioid receptor delta 1 Inhibitory activity against forskolin-stimulated cAMP production in chinese hamster ovary cells expressing Opioid receptor delta 1	[PMID: 9873480]
CHO-K1	EC₅₀	6.34 nM Compound: DPDPE	Agonist activity at human delta opioid receptor transfected in CHOK1 cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at human delta opioid receptor transfected in CHOK1 cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 23659286]
HEK293	IC₅₀	1.12 nM Compound: DPDPE	Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK293 cells Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK293 cells	[PMID: 16989522]
HEK293	IC₅₀	1.2 nM Compound: DPDPE	Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cell membrane Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cell membrane	[PMID: 17388627]
HEK293	IC₅₀	1.12 nM Compound: DPDPE	Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cell membranes Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cell membranes	[PMID: 17451272]
HEK293	IC₅₀	1.2 nM Compound: DPDPE	Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cells Displacement of [125I]deltorphin 2 from human recombinant delta opioid receptor expressed in HEK cells	[PMID: 18039010]
HEK293	EC₅₀	15 nM Compound: 14, DPDPE	Activity at delta opioid receptor expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding Activity at delta opioid receptor expressed in HEK293 cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 19683449]
HEK293	IC₅₀	1.2 nM Compound: DPDPE	Displacement of [3H]deltorphin-2 from human delta opioid receptor expressed in HEK293 cell membrane Displacement of [3H]deltorphin-2 from human delta opioid receptor expressed in HEK293 cell membrane	[PMID: 20426456]
HEK293	IC₅₀	0.14 nM Compound: DPDPE	Agonist activity at delta opioid expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation Agonist activity at delta opioid expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation	[PMID: 20599386]
HEK293	EC₅₀	0.14 nM Compound: DPDPE, c[D-Pen2,DPen5] enkephalin	Agonist activity at human DOR expressed in HEK293 cell membrane assessed as inhibition of forskolin-induced cAMP accumulation by by liquid scintillation counting Agonist activity at human DOR expressed in HEK293 cell membrane assessed as inhibition of forskolin-induced cAMP accumulation by by liquid scintillation counting	[PMID: 21978284]
HEK293	ED₅₀	6.81 nM Compound: DPDPE	Agonist activity at human delta opioid receptor expressed in HEK293 cells by [35S]GTP binding assay Agonist activity at human delta opioid receptor expressed in HEK293 cells by [35S]GTP binding assay	[PMID: 22325949]
HEK293	EC₅₀	0.557 nM Compound: DPDPE	Agonist activity at Myc-tagged delta-type opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by liquid scintillation counting analysis Agonist activity at Myc-tagged delta-type opioid receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins by liquid scintillation counting analysis	[PMID: 23477419]
HEK293	IC₅₀	0.0009 μM Compound: DPDPE	Agonist activity at human delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 mins by HTRF assay Agonist activity at human delta opioid receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP production after 30 mins by HTRF assay	[PMID: 25087049]
HEK293	IC₅₀	1.6 nM Compound: DPDPE	Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method	[PMID: 27607020]
HEK293	IC₅₀	1.6 nM Compound: DPDPE	Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method Agonist activity at human MOR expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation by EIA method	[PMID: 27607020]
HEK293	IC₅₀	0.0003 μM Compound: DPDPE	Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay	[PMID: 27776274]
HEK293	IC₅₀	0.3 nM Compound: DPDPE	Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay Agonist activity at human DOR expressed in HEK293 cells assessed as inhibition of forskolin-induced adenylyl cyclase-mediated cAMP accumulation after 30 mins by HTRF assay	[PMID: 27776274]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	>50 μM Compound: Enkephalin (DPDPE)	TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells	[PMID: 12699389]

In Vivo

DPDPE (70 or 140 nM; i.c.v.; once) shows anticonvulsant effects in rats^[1].
DPDPE (0.78–25 μg/mouse; i.c.v.; once) enhances bicuculline-induced convulsions in mouse^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague-Dawley rats (225-275 g)^[1]
Dosage:

70 or 140 nM followed by a 2 μl saline flush
Administration:

Intracerebroventricular injection
Result:

Resulted in a significant, dose-dependent increase in the convulsive threshold to flurothyl.

Animal Model:

Male ddY mice weighing 20–30 g, pretreated with 1.5 mg/kg bicuculline (subcutaneous injection)^[2].
Dosage:

0.78–25 μg/mouse, once
Administration:

Intracerebroventricular injection
Result:

Caused a dose-dependent increase in the proportion of mice exhibiting convulsions.

Chemical Information

CAS No. 88373-73-3
Appearance Solid
Molecular Weight 645.79
Formula C₃₀H₃₉N₅O₇S₂
Color White to off-white
Sequence

Tyr-{Pen}-Gly-Phe-{Pen} (Disulfide bridge:Pen2-Pen5)
Sequence Shortening

Y{Pen}GF{Pen} (Disulfide bridge:Pen2-Pen5)
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (2)

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J Med Chem

2026 Mar 5;69(6):6508-6527.
University of Padua

202.

Solvent & Solubility

In Vitro:

H₂O : 10 mg/mL (15.48 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5485 mL

7.7425 mL

15.4849 mL

5 mM

0.3097 mL

1.5485 mL

3.0970 mL

10 mM

0.1548 mL

0.7742 mL

1.5485 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. F C Tortella, et al. Anticonvulsant Effects of Mu (DAGO) and Delta (DPDPE) Enkephalins in Rats. Peptides [Content Brief]

[2]. Yajima Y, et al. Effects of differential modulation of mu-, delta- and kappa-opioid systems on bicuculline-induced convulsions in the mouse. Brain Res. 2000 Apr 17;862(1-2):120-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.5485 mL	7.7425 mL	15.4849 mL	38.7123 mL
	5 mM	0.3097 mL	1.5485 mL	3.0970 mL	7.7425 mL
	10 mM	0.1548 mL	0.7742 mL	1.5485 mL	3.8712 mL
	15 mM	0.1032 mL	0.5162 mL	1.0323 mL	2.5808 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DPDPE

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

Neurotransmitter Receptor Compound Library

Peptide Library

Antidepressant Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Peptide Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) DPDPE

All Opioid Receptor Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

DPDPE

GPCR/G Protein Compound Library

Neuronal Signaling Compound Library

Neurotransmitter Receptor Compound Library

Peptide Library

Antidepressant Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Activators Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Peptide Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) DPDPE

All Opioid Receptor Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

DPDPE Related Classifications

Cat. No.: HY-P1334