DPM-1001 trihydrochloride
Based on 1 Customer Validation
DPM-1001 trihydrochloride is a potent, specific, orally active and non-competitive inhibitor of protein-tyrosine phosphatase (PTP1B) with an IC50 of 100 nM. DPM-1001 trihydrochloride is an analog of the specific PTP1B inhibitor MSI-1436. DPM-1001 trihydrochloride has anti-diabetic property.
For research use only. We do not sell to patients.
- Purity: 99.69%
- Formula: C35H60Cl3N3O3
- Molecular Weight:677.23
-
Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
DPM-1001 trihydrochloride inhibits the short form of PTP1B reversibly, whereas PTP1B(1-405) remained inactive over an extended period of time. DPM-1001 is against PTP1B(1-405) with no pre-incubation, the IC50 value for PTP1B(1-405) is 600 nM. However, after a 30-min pre-incubation, the potency is improved to 100 nM. In contrast, there is no obvious time-dependent change in the IC50 value for PTP1B(1-321)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:18 weeks of age, high-fat diet (HFD)-fed obese male mice (C57bl6/J)
-
Dosage:5 mg/kg
-
Administration:Oral or intraperitoneal administration; 5 mg/kg; once daily; 50 day
-
Result:Led to an 5% decrease in body weight.Improved glucose tolerance and insulin sensitivity in glucose tolerance and insulin tolerance in vivo.
Chemical Information
-
Appearance Solid
-
Molecular Weight 677.23
-
Formula C35H60Cl3N3O3
-
Color Off-white to light yellow
-
SMILES
O[C@H]1[C@]2([H])[C@](CC[C@]3(C)[C@@]2([H])CC[C@@]3([H])[C@H](C)CCC(OC)=O)([H])[C@]4(C)[C@@](C[C@H](NCCCCNCC5=CC=CC=N5)CC4)([H])C1.Cl.Cl.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
DMSO : 80 mg/mL (118.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 33.33 mg/mL (49.22 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.4766 mL | 7.3830 mL | 14.7660 mL | 36.9151 mL |
| 5 mM | 0.2953 mL | 1.4766 mL | 2.9532 mL | 7.3830 mL | |
| 10 mM | 0.1477 mL | 0.7383 mL | 1.4766 mL | 3.6915 mL | |
| 15 mM | 0.0984 mL | 0.4922 mL | 0.9844 mL | 2.4610 mL | |
| 20 mM | 0.0738 mL | 0.3692 mL | 0.7383 mL | 1.8458 mL | |
| 25 mM | 0.0591 mL | 0.2953 mL | 0.5906 mL | 1.4766 mL | |
| 30 mM | 0.0492 mL | 0.2461 mL | 0.4922 mL | 1.2305 mL | |
| 40 mM | 0.0369 mL | 0.1846 mL | 0.3692 mL | 0.9229 mL | |
| DMSO | 50 mM | 0.0295 mL | 0.1477 mL | 0.2953 mL | 0.7383 mL |
| 60 mM | 0.0246 mL | 0.1231 mL | 0.2461 mL | 0.6153 mL | |
| 80 mM | 0.0185 mL | 0.0923 mL | 0.1846 mL | 0.4614 mL | |
| 100 mM | 0.0148 mL | 0.0738 mL | 0.1477 mL | 0.3692 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DPM-1001 trihydrochloride Related Classifications
HY-121515A Related Classifications
Powered by Bioz