Entacapone sodium salt
Based on 8 publication(s) in Google Scholar
Entacapone sodium salt is a potent, reversible, peripherally acting and orally active catechol-O-methyltransferase (COMT) inhibitor. Entacapone sodium salt inhibits COMT from rat brain, erythrocytes and liver with IC50 values of 10 nM, 20 nM, and 160 nM, respectively. Entacapone sodium salt is selective for COMT over other catecholamine metabolizing enzymes, including MAO-A, MAO-B, phenolsulphotransferase M (PST-M) and PST-P (IC50s>50 µM). Entacapone sodium salt can be used for the research of Parkinson's disease. Entacapone sodium salt serves as as a inhibit of FTO demethylation with an IC50 of 3.5 μM, can be used for the research of metabolic disorders.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 1047659-02-8
- 화학식: C14H14N3NaO5
- 분자량:327.27
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
-
HY-14280
EntacaponeCOMT Inhibitor -
HY-14896
OpicaponeCOMT Inhibitor -
HY-14280R
Entacapone (Standard)COMT Inhibitor -
HY-106842
NitecaponeCOMT Inhibitor -
HY-106405
NebicaponeCOMT Inhibitor -
HY-14896R
Opicapone (Standard)COMT Inhibitor -
HY-133068
5-Hydroxyferulic acidHydroxycinnamic Acid -
HY-106405R
Nebicapone (Standard)COMT Inhibitor
Publications Citing Use of MedChemExpress (MCE) Entacapone sodium salt
More- Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
- Sensor Actuat B-Chem. 2021, 129983.
- Biochem Pharmacol. 2025 Oct:240:117120. [Abstract]
- Chin J Chem. 2023 Dec 27.
- FASEB J. 2022 Jul;36(7):e22399. [Abstract]
- J Pharm Pharmacol. 2025 Dec 7:rgaf118. [Abstract]
- J Solid State Electrochem. 2025 Jan 17.
- Eur J Drug Metab Pharmacokinet. 2022 Sep;47(5):639-652. [Abstract]
Customer Validation & Images
-
Bio/Physico-chemical Assay
-
Flow Cytometry
-
IF
-
Bio/Physico-chemical Assay
-
In Vivo Efficacy Study
Biological Activity
제품 설명
IC50 & Target
IC50: 10 nM (rat brain COMT); 20 nM (rat erythrocyte COMT); 160 nM (rat liver COMT)[1]
In Vitro
Entacapone sodium salt (50 μM, 48 hours) enhances the amount of m6A on mRNA in Hep-G2 cells. It does not show any inhibitory effect on the enzymatic activity of the RNA m6A demethylase AlkB homolog 5 (ALKBH5) or the ten-eleven translocation methylcytosine dioxygenase 1 (TET1), nor does it alter the DNA methylation or histone methylation patterns in Entacapone sodium salt -treated Hep-G2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 1047659-02-8
-
분자량 327.27
-
화학식 C14H14N3NaO5
-
SMILES
O=C(N(CC)CC)/C(C#N)=C/C1=CC([N+]([O-])=O)=C([O-])C(O)=C1.[Na+]
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
Cancer Res
Targeting Catechol-O-Methyltransferase Induces Mitochondrial Dysfunction and Enhances the Efficacy of Radiotherapy in Glioma. [Abstract]2024 Nov 4;84(21):3640-3656. PMID: 39088832
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
The rate of NADH to NAD+ conversion rate measuring the mitochondrial complex I activity using mitochondrial extracts from U87 cells treated with DMSO, 50 μmol/L Entacapone, or 100 μmol/L Tolcapone for 24 hours.
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
ROS flow cytometry assay comparing the ROS production of U87 cells treated with DMSO (control) or 50 μmol/L Entacapone for 24 hours.
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
Immunofluorescence showing the localization of the mitochondria (as detected by HSP60) and dsRNA (as detected by J2) in the cytoplasm of VC, COMT-KO, Act-D–treated VC, Act-D–treated COMT-KO U87 cells (top), and wild-type U87 cells treated with DMSO or 50 µmol/L Entacapone for 24 hours (bottom).
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
Quantification of the percentage of macrophages that phagocytosed CT2A cells treated with DMSO, 50 µmol/L Entacapone, or 50 µmol/L Tolcapone. CT2A cells were pretreated with COMT inhibitors for 24 hours prior to coculturing with macrophages.
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
The drug treatment and irradiation schedule in mice bearing subcutaneous CT2A tumors. Vehicle and 30 mg/kg Entacapone were administered intraperitoneally daily from day 3 to day 35. The tumor-bearing right flank was irradiated on day 10 post tumor cell inoculation. IP, intraperitoneal injection; q.d., every day; IR, irradiation.
Entacapone sodium salt purchased from MedChemExpress. Usage Cited in: Cancer Res. 2024 Nov 4;84(21):3640-3656. [Abstract]
Tumor growth of C57BL/6 mice (n = 6) subcutaneously implanted with 5 × 105 CT2A cells and received Entacapone and Radiotherapy treatments.
-
-
Biochem Pharmacol
Dual inhibition of EGR1/STAT3 transcriptional hubs suppresses macrophage-driven liver fibrosis: A multi-omics-guided drug repurposing strategy. [Abstract]2025 Oct:240:117120. PMID: 40623460 -
-
FASEB J
2022 Jul;36(7):e22399. PMID: 35691001 -
J Pharm Pharmacol
High-dose carbidopa achieves sustained levodopa pharmacokinetics and efficacy in Parkinson's disease models. [Abstract]2025 Dec 7:rgaf118. PMID: 41353579 -
-
Eur J Drug Metab Pharmacokinet
Evaluation of an Ussing Chamber System Equipped with Rat Intestinal Tissues to Predict Intestinal Absorption and Metabolism in Humans. [Abstract]2022 Sep;47(5):639-652. PMID: 35733077
Protocol
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Human pluripotent stem cell midbrain dopaminergic neuron differentiation
Human pluripotent stem cells are directed toward midbrain dopaminergic neurons by first inducing a neural floor-plate-like progenitor state, then patterning cells with ventralizing SHH signaling and midbrain/WNT-FGF cues, and finally maturing progenitors into neurons expressing dopaminergic markers such as TH, NURR1/NR4A2, PITX3, DAT/SLC6A3, VMAT2/SLC18A2, GIRK2/KCNJ6, FOXA2, LMX1A, and EN1. The main readouts are loss of pluripotency, acquisition of FOXA2+/LMX1A+ midbrain floor-plate progenitors, emergence of βIII-tubulin+/MAP2+ neurons, and production of TH+ dopaminergic neurons with molecular, dopamine-release, and electrophysiological features of midbrain dopaminergic identity.
순도&문서
References
[1]. E Nissinen, et al. Biochemical and pharmacological properties of a peripherally acting catechol-O-methyltransferase inhibitor Entacapone sodium salt . Naunyn Schmiedebergs Arch Pharmacol. 1992 Sep;346(3):262-6. [Content Brief]
[2]. Shiming Peng, et al. Identification of Entacapone sodium salt as a chemical inhibitor of FTO mediating metabolic regulation through FOXO1. Sci Transl Med [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Entacapone sodium salt Related Classifications
HY-14280A Related Classifications
Powered by Bioz