Procyanidin B2
Based on 17 publication(s) in Google Scholar
Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 29106-49-8
- Formula: C30H26O12
- Molecular Weight:578.52
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Procyanidin B2
More- Food Chem. 2025 May 31:489:144992. [Abstract]
- Food Chem. 2023 Mar 30;405(Pt A):134807. [Abstract]
- Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
- Phytomedicine. 2025 May:140:156549. [Abstract]
- Food Res Int. 2019 May;119:187-195. [Abstract]
- Antioxidants (Basel). 2021 Jun 5;10(6):916. [Abstract]
- J Ethnopharmacol. 2025 Mar 26:344:119528. [Abstract]
- Food Funct. 2020 Dec 1;11(12):10493-10505. [Abstract]
- Int J Mol Sci. 2022 Jul 14;23(14):7769. [Abstract]
- Int Immunopharmacol. 2022 Dec;113(Pt A):109336. [Abstract]
- BMC Complement Med Ther. 2021 Feb 24;21(1):76. [Abstract]
- Andrology. 2020 Dec 5.
- Int J Food Sci Nutr. 2020 Sep;71(6):715-725. [Abstract]
- Mol Immunol. 2022 May 7;147:126-135. [Abstract]
- Theriogenology. 2025 Jun 25:246:117555. [Abstract]
- Exp Ther Med. 2022 Jun 7;24(2):496. [Abstract]
- Massey University. 2023 Aug.
Customer Validation & Images
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Histological Imaging/Staining
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Flow Cytometry
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WB
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IHC
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Cell Imaging/Staining
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | ED50 |
>10 μg/mL
Compound: 48
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Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| HCT-8 | ED50 |
>10 μg/mL
Compound: 48
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Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| HUVEC | IC50 |
3.8 μM
Compound: 44
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Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells
Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells
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10.1007/s00044-012-0353-y |
| KB | ED50 |
>10 μg/mL
Compound: 48
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Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| MT4 | CC50 |
135.1 μg/mL
Compound: 2, procyanidin B2
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Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
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[PMID: 10425115] |
| MT4 | IC50 |
17.2 μg/mL
Compound: 2, procyanidin B2
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Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
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[PMID: 10425115] |
| TE-671 | ED50 |
>10 μg/mL
Compound: 48
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Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
Procyanidin B2 shows antiproliferative activity to MCF-7 cells, with an IC50 of 19.21 μM. However, Procyanidin B2 exhibits no effect on DNA-ladder formation[1]. Procyanidin B2 (0.1, 1, 2 μM) inhibits the activation of pyrin domain containing 3 (NLRP3) inflammasome in human umbilical vein ECs (HUVECs), and the inhibition is via suppression of AP-1 activity, and such effect can be abolished by overexpression of c-Jun. Procyanidin B2 (2 μM for 12 h) also reduces ROS in HUVECs[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 29106-49-8
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Appearance Solid
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Molecular Weight 578.52
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Formula C30H26O12
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Color Light yellow to light brown
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SMILES
O[C@H]1[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3[C@@H]1C4=C5C(C[C@@H](O)[C@@H](C6=CC=C(O)C(O)=C6)O5)=C(O)C=C4O
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Synonyms
Proanthocyanidin B2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (17)
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Journal Impact Factor
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Most Recent
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Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Food Chem
Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]2023 Mar 30;405(Pt A):134807. PMID: 36370576 -
Cell Death Dis
Mitochondrial PKM2 deacetylation by procyanidin B2-induced SIRT3 upregulation alleviates lung ischemia/reperfusion injury. [Abstract]2022 Jul 11;13(7):594. PMID: 35821123
Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
The indicated dosages of PCB2 (Procyanidin B2) were injected intraperitoneally for three consecutive days before the operation. Representative images of the H&E-stained lung tissue.
Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. A representative FACS scatter plot of Annexin V and propidium iodide dual labeling is shown.
Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. The expression of Bcl-2 protein in lung tissue was investigated.
Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
C57BL/6 mice were intraperitoneally injected with 30 mg/kg PCB2 (Procyanidin B2) for three consecutive days. SIRT3 immunohistochemical staining was performed.
Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]
I/R mice were subjected to 1 hour of ischemia and 2 hours of reperfusion to induce lung I/R injury. Control mice underwent sham surgery. Preoperative intraperitoneal injections of PCB2 (Procyanidin B2: 30 mg/kg) were administered for three consecutive days prior to surgery. Representative images of A549 cells stained with TUNEL; cell nuclei were stained with DAPI.
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Phytomedicine
2025 May:140:156549. PMID: 40023969 -
Food Res Int
Slow starch digestion in the rice cooked with adzuki bean: Contribution of procyanidins and the oxidation products. [Abstract]2019 May;119:187-195. PMID: 30884648 -
Antioxidants (Basel)
Procyanidin B2 Reduces Vascular Calcification through Inactivation of ERK1/2-RUNX2 Pathway. [Abstract]2021 Jun 5;10(6):916. PMID: 34198832 -
J Ethnopharmacol
Procyanidin B2 attenuates microvascular dysfunction in diabetic retinopathy via inhibition of caspase-1/GSDMD mediated pyroptosis. [Abstract]2025 Mar 26:344:119528. PMID: 39986358 -
Food Funct
Grape seed procyanidins suppress the apoptosis and senescence of chondrocytes and ameliorates osteoarthritis via the DPP4-Sirt1 pathway. [Abstract]2020 Dec 1;11(12):10493-10505. PMID: 33175932 -
Int J Mol Sci
Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells. [Abstract]2022 Jul 14;23(14):7769. PMID: 35887117 -
Int Immunopharmacol
Procyanidin B2 ameliorates the progression of osteoarthritis: An in vitro and in vivo study. [Abstract]2022 Dec;113(Pt A):109336. PMID: 36274486 -
BMC Complement Med Ther
Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway. [Abstract]2021 Feb 24;21(1):76. PMID: 33627124 -
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Int J Food Sci Nutr
Contribution of amylose-procyanidin complexes to slower starch digestion of red-colored rice prepared by cooking with adzuki bean. [Abstract]2020 Sep;71(6):715-725. PMID: 31986936 -
Mol Immunol
Preconditioning with procyanidin B2 protects MAC-T cells against heat exposure-induced mitochondrial dysfunction and inflammation. [Abstract]2022 May 7;147:126-135. PMID: 35537356 -
Theriogenology
Procyanidin B2 improves the in vitro maturation of porcine oocytes and subsequent embryonic development. [Abstract]2025 Jun 25:246:117555. PMID: 40580853 -
Exp Ther Med
Procyanidin B2 suppresses hyperglycemia-induced renal mesangial cell dysfunction by modulating CAV-1-dependent signaling. [Abstract]2022 Jun 7;24(2):496. PMID: 35837062 -
Solvent & Solubility
H2O : 66.67 mg/mL (115.24 mM; Need ultrasonic)
DMSO : ≥ 50 mg/mL (86.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (86.43 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
MCF-7 cells are grown in 5 mL of RPMI 1640 medium supplemented with 20% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells are maintained at 37°C in a humidified atmosphere of 5% CO2 in air and subcultured every 3 days. Exponentially growing cells are plated at a seeding density of 2×104 cells/mL, in 96-well plates or in dishes. After overnight incubation to allow for attachment, the cells are exposed for 24 or 48 h to various concentrations of Procyanidin B2 (0.5; 1.0; 5.0; 10.0; 25.0 and 50.0 μM) diluted in distilled water. Two millimolar cyclophosphamide (CP) is used as the positive control and solutions are sterilised by filtration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
To observe the effect of Procyanidin B2 on infarct size or brain edema, 40 rats are randomLy separated into five groups, and each group is administered with vehicle (equivalent dose of 0.9% saline administered via gavage) or 40, 20, or 10 mg/kg of Procyanidin B2, respectively. To elucidate the effect of Procyanidin B2 on blood-brain barrier (BBB) permeability, the rats (n = 6 per group for Evans blue extravasation and n = 4 per group for IgG leakage) are randomLy separated into two groups: vehicle and Procyanidin B2 (40 mg/kg). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after middle cerebral artery occlusion (MCAO). For other observations, including immunohistological staining and western blot analysis, rats are randomLy separated into three groups: sham-operated, vehicle, and Procyanidin B2 (n = 4 per group). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after MCAO. To elucidate the improvement in neurological function after ischemic stroke in rats, the rats (n = 8 per group) undergo neurobehavioral assays to evaluate the functional outcome after administration of Procyanidin B2 (40 mg/kg) once a day starting at 24 h after MCAO. The neurological deficits are assessed at 1, 3, 7, 11, and 14 days after MCAO[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5. [Content Brief]
[2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606. [Content Brief]
[3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL | 43.2137 mL |
| 5 mM | 0.3457 mL | 1.7285 mL | 3.4571 mL | 8.6427 mL | |
| 10 mM | 0.1729 mL | 0.8643 mL | 1.7285 mL | 4.3214 mL | |
| 15 mM | 0.1152 mL | 0.5762 mL | 1.1524 mL | 2.8809 mL | |
| 20 mM | 0.0864 mL | 0.4321 mL | 0.8643 mL | 2.1607 mL | |
| 25 mM | 0.0691 mL | 0.3457 mL | 0.6914 mL | 1.7285 mL | |
| 30 mM | 0.0576 mL | 0.2881 mL | 0.5762 mL | 1.4405 mL | |
| 40 mM | 0.0432 mL | 0.2161 mL | 0.4321 mL | 1.0803 mL | |
| 50 mM | 0.0346 mL | 0.1729 mL | 0.3457 mL | 0.8643 mL | |
| 60 mM | 0.0288 mL | 0.1440 mL | 0.2881 mL | 0.7202 mL | |
| 80 mM | 0.0216 mL | 0.1080 mL | 0.2161 mL | 0.5402 mL | |
| H2O | 100 mM | 0.0173 mL | 0.0864 mL | 0.1729 mL | 0.4321 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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