TMB monosulfate
Based on 8 publication(s) in Google Scholar
TMB monosulfate is the monosulfate form of TMB (HY-15930). TMB monosulfate is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB monosulfate can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB monosulfate can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.
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- Pureza: 99.88%
- No. CAS: 54827-18-8
- Fòrmula: C16H22N2O4S
- Peso molecular:338.42
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TMB monosulfate
More- Chem Eng J. 2025 Nov 24;526:171230.
- J Control Release. 2025 May 10:381:113612. [Abstract]
- Nano Res. 2026 Feb 6.
- Int J Biol Macromol. 2025 Sep 14;328(Pt 1):147690. [Abstract]
- Mater Des. 2026 Apr 19;266:116048.
- View. 2026 Mar 22.
- J Invertebr Pathol. 2024 May 14:205:108129. [Abstract]
- SSRN. 2026 Jun 11.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Actividad biológica
TMB monosulfate can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 54827-18-8
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Appearance Solid
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Peso molecular 338.42
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Fòrmula C16H22N2O4S
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Color White to off-white
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SMILES
NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.O=S(O)(O)=O
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Synonyms
BM blue monosulfate; Sure Blue TMB monosulfate
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (8)
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Journal Impact Factor
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Most Recent
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TMB monosulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
A diagram showing the chemical reaction formula of TMB assay, MB assay, and DTNB assay.
TMB monosulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.
The results of TMB assay.
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J Control Release
Synergistic photothermal-sonodynamic therapy for antibacterial and immune reprogramming in chronic osteomyelitis. [Abstract]2025 May 10:381:113612. PMID: 40073945 -
TMB monosulfate purchased from MedChemExpress. Usage Cited in: Nano Res. 2026 Feb 6.
Ov-ZBC showed strong characteristic peaks, indicating its higher photocatalytic ROS generation capability. The results of TMB and MB chromogenic reactions shown in Fig.
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Int J Biol Macromol
Construction and characterization of a novel single-chain variable fragment against Bacillus thuringiensis vegetative insecticidal protein. [Abstract]2025 Sep 14;328(Pt 1):147690. PMID: 40957516 -
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J Invertebr Pathol
Characterization of the individual domains of the Bacillus thuringiensis Cry2Aa implicates Domain I as a possible binding site to Helicoverpa armigera. [Abstract]2024 May 14:205:108129. PMID: 38754546 -
Solvente y solubilidad
DMSO : ≥ 28 mg/mL (82.74 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2.78 mg/mL (8.21 mM; ultrasonic and warming and heat to 60°C)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. [Content Brief]
[2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9549 mL | 14.7745 mL | 29.5491 mL | 73.8727 mL |
| 5 mM | 0.5910 mL | 2.9549 mL | 5.9098 mL | 14.7745 mL | |
| DMSO | 10 mM | 0.2955 mL | 1.4775 mL | 2.9549 mL | 7.3873 mL |
| 15 mM | 0.1970 mL | 0.9850 mL | 1.9699 mL | 4.9248 mL | |
| 20 mM | 0.1477 mL | 0.7387 mL | 1.4775 mL | 3.6936 mL | |
| 25 mM | 0.1182 mL | 0.5910 mL | 1.1820 mL | 2.9549 mL | |
| 30 mM | 0.0985 mL | 0.4925 mL | 0.9850 mL | 2.4624 mL | |
| 40 mM | 0.0739 mL | 0.3694 mL | 0.7387 mL | 1.8468 mL | |
| 50 mM | 0.0591 mL | 0.2955 mL | 0.5910 mL | 1.4775 mL | |
| 60 mM | 0.0492 mL | 0.2462 mL | 0.4925 mL | 1.2312 mL | |
| 80 mM | 0.0369 mL | 0.1847 mL | 0.3694 mL | 0.9234 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TMB monosulfate Related Classifications
HY-15930C Related Classifications
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