HKGreen-4I

Guide (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
1. Preparation of HKGreen-4I working solution
1.1 Preparation of stock solution
Dissolve 1 mg HKGreen-4I in 150 μL anhydrous DMSO to prepare a 10 mM stock solution.
Note: It is recommended to aliquot the HKGreen-4I stock solution and store at -20°C or -80°C, protected from light.
1.2 Preparation of working solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a 1-10 μM HKGreen-4I working solution.
Note: Please adjust the concentration of the HKGreen-4I working solution according to actual conditions and prepare fresh before use.
2. Cell staining (suspension cells)
2.1 Collect cells by centrifugation, wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×10⁶/mL.
2.2 Add 1 mL HKGreen-4I working solution and incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.4 Wash the cells twice with PBS for 5 minutes each time.
2.5 Resuspend the cells in 1 mL serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate excess medium.
3.3 Add 100 μL staining working solution, gently shake to ensure complete coverage of the cells, and incubate for 5-30 minutes.
3.4 Remove the staining working solution and wash with culture medium 2-3 times for 5 minutes each time, then observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.