2. GPCR/G Protein
  3. GLP Receptor
  4. H-Trp-Tyr-OH

H-Trp-Tyr-OH is an orally active tryptophan-tyrosine dipeptide with blood-brain barrier permeability. H-Trp-Tyr-OH exerts physiological regulatory effects by stimulating enteroendocrine cells to secrete glucagon-like peptide GLP-1. In mouse models of tauopathies, H-Trp-Tyr-OH inhibits tau phosphorylation, reduces the level of neurofibrillary tangles, increases dopamine turnover, upregulates synapsin expression, and elevates cecal short-chain fatty acid levels, thereby improving behavioral deficits and extending lifespan. H-Trp-Tyr-OH can be used in research related to impaired glucose tolerance and tauopathies.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

H-Trp-Tyr-OH

H-Trp-Tyr-OH Chemical Structure

CAS No. : 19653-76-0

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Based on 1 publication(s) in Google Scholar

H-Trp-Tyr-OH Related Antibodies

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Description

H-Trp-Tyr-OH is an orally active tryptophan-tyrosine dipeptide with blood-brain barrier permeability. H-Trp-Tyr-OH exerts physiological regulatory effects by stimulating enteroendocrine cells to secrete glucagon-like peptide GLP-1. In mouse models of tauopathies, H-Trp-Tyr-OH inhibits tau phosphorylation, reduces the level of neurofibrillary tangles, increases dopamine turnover, upregulates synapsin expression, and elevates cecal short-chain fatty acid levels, thereby improving behavioral deficits and extending lifespan. H-Trp-Tyr-OH can be used in research related to impaired glucose tolerance and tauopathies[1][2][3].

Cellular Effect
Cell Line Type Value Description References
MDCK IC50
0.08 mM
Compound: 69
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
Binding affinity to human PEPT1 assessed as inhibition of [14C]Gly-Sar uptake in MDCK cells
[PMID: 16759105]
In Vitro

H-Trp-Tyr-OH (1-5 mg/mL; 60 min) potently stimulates GLP-1 secretion from GLUTag cells, with the highest activity observed at 5 mg/mL (the induced GLP-1 level is approximately 6-fold higher than that of the blank control), and exhibits no cytotoxicity against GLUTag cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H-Trp-Tyr-OH Related Antibodies

ELISA Assay[2]

Cell Line: murine enteroendocrine GLUTag cells
Concentration: 1 mg/mL; 2 mg/mL; 5 mg/mL; 1.85 mg/mL
Incubation Time: 60 min
Result: Induced a GLP-1 secretion level ~6-fold relative to blank control at 5 mg/mL.
Induced a GLP-1 level ~1-fold relative to blank control at 1 mg/mL.
Induced a GLP-1 level ~2-fold relative to blank control at 2 mg/mL.
Induced a GLP-1 level ~1.2-fold relative to blank control at 1.85 mg/mL.
Remained at baseline LDH release levels, showing no cytotoxicity.
In Vivo

H-Trp-Tyr-OH (0.05% w/w; p.o.; continuous dietary intake) improves tauopathy-related behavioral deficits, increases dopamine turnover and synaptic marker levels, reduces tau phosphorylation, elevates cecal short-chain fatty acids, and significantly increases survival in PS19 tauopathy mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B6;C3-Tg mice (Prnp-MAPT*P301S)PS19Vle/J (PS19 transgenic; male and female, 2.5 months old, tauopathy model); wild-type littermates (male and female, 2.5 months old)[3]
Dosage: 0.05% w/w
Administration: p.o.; continuous dietary intake; 6.5 months (male); until 53 weeks of age (female)
Result: Reduced total distance traveled in the open field test at 5 and 7 months of age, normalizing locomotor hyperactivity.
Increased the number of NeuN-positive cells in the entorhinal cortex and elevated cortical synaptophysin levels in male mice.
Increased dopamine turnover (DOPAC/dopamine ratio and (DOPAC + HVA)/dopamine ratio) in the frontal cortex of male mice.
Reduced hippocampal pTau levels (113.1 vs control 162.2) and cortical pTau/tTau ratio (0.02 vs control 0.04) in male mice; reduced pTau-positive region in the spinal cord.
Achieved a 67% survival ratio at 53 weeks of age in female mice, significantly higher than control's 38%.
Increased cecal acetic acid and total short-chain fatty acid levels in male mice.
Molecular Weight

367.40

Formula

C20H21N3O4
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Trp-Tyr
Sequence Shortening

WY
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (68.05 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7218 mL 13.6091 mL 27.2183 mL
5 mM 0.5444 mL 2.7218 mL 5.4437 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.7218 mL 13.6091 mL 27.2183 mL 68.0457 mL
5 mM 0.5444 mL 2.7218 mL 5.4437 mL 13.6091 mL
10 mM 0.2722 mL 1.3609 mL 2.7218 mL 6.8046 mL
15 mM 0.1815 mL 0.9073 mL 1.8146 mL 4.5364 mL
20 mM 0.1361 mL 0.6805 mL 1.3609 mL 3.4023 mL
25 mM 0.1089 mL 0.5444 mL 1.0887 mL 2.7218 mL
30 mM 0.0907 mL 0.4536 mL 0.9073 mL 2.2682 mL
40 mM 0.0680 mL 0.3402 mL 0.6805 mL 1.7011 mL
50 mM 0.0544 mL 0.2722 mL 0.5444 mL 1.3609 mL
60 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1341 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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H-Trp-Tyr-OH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Trp-Tyr-OH19653-76-0GLP Receptorshort-chain fatty acidtauopathy mouse modelsenteroendocrine cellsneurofibrillary tangledopamine turnoverglucagon-like peptide-1GLUTag cellsPS19 tauopathy micesynaptophysintau phosphorylationInhibitorinhibitorinhibit

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