HER2-targeted peptide H10
HER2-targeted peptide H10 is a HER2-targeting peptide with a Kd value of 30.4 nM. HER2-targeted peptide H10 specifically accumulates in HER2-positive tumor tissues of xenograft mice, when conjugated with nanoparticles. HER2-targeted peptide H10 can be used for studies related to HER2-positive breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 1802710-54-8
- Formula: C50H83N17O12
- Molecular Weight:1114.30
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Biological Activity
The HER2-targeted peptide H10 (H10) (5.0 × 10-5 M; 30 min at 4 °C) specifically binds to SKBR-3 cells with high HER2 expression, but does not bind to 293A cells with low HER2 expression; this binding activity completely disappears in SKBR-3 cells with downregulated HER2 expression[1].
The HER2-targeted peptide H10 (0.1-100 μM) exhibits low cytotoxicity, with cell survival rates approaching 100% across the concentration range of 0.1 μM to 100 μM in the tested cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human breast cancer SKBR-3 cells, human embryonic kidney 293A cells, HER2-downregulated RNAi SKBR-3 cells
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Concentration:5.0 × 10-5 M (FITC-labeled H10); 1 mM (Hoechst 33342)
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Incubation Time:30 min (at 4 °C)
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Result:Showed strong green fluorescence signal on the membrane of SKBR-3 cells, but no significant binding to 293A cells.
Drastically reduced green fluorescence signal in HER2-downregulated RNAi SKBR-3 cells.
Measured a 53.32% binding rate of FITC-H10 to SKBR-3 cells via flow cytometry.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mice (8-week-old female)[1]
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Dosage:2 μM
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Administration:i.v.; single dose
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Result:Showed specific accumulation at the tumor site in in vivo imaging.
Confirmed high fluorescence intensity in tumor tissue, with relatively lower accumulation in liver and spleen compared to H6-Qdot705.
Reached quantitative ex vivo fluorescence intensity of ~65,000 ×106 photons/cm2/s in tumors.
Chemical Information
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CAS No. 1802710-54-8
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Molecular Weight 1114.30
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Formula C50H83N17O12
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Sequence
Lys-Leu-Arg-Leu-Glu-Trp-Asn-Arg
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Sequence Shortening
KLRLEWNR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HER2-targeted peptide H10 Related Classifications
HY-P11760 Related Classifications
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