1. Immunology/Inflammation
  2. NOD-like Receptor (NLR)
  3. JC-171

JC-171 

Cat. No.: HY-117432
Handling Instructions

JC-171 is a selective NLRP3 inflammasome inhibitor, with an IC50 of 8.45 μM for inhibiting LPS/ATP-induced interleukin-1β (IL-1β) release from J774A.1 macrophages.

For research use only. We do not sell to patients.

JC-171 Chemical Structure

JC-171 Chemical Structure

CAS No. : 2112809-98-8

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Description

JC-171 is a selective NLRP3 inflammasome inhibitor, with an IC50 of 8.45 μM for inhibiting LPS/ATP-induced interleukin-1β (IL-1β) release from J774A.1 macrophages[1].

In Vitro

JC-171 (0-100 μM) blocks NLRP3 inflammasome activation and IL-1β production in primary macrophages dose dependently[1].

Cell Viability Assay[1]

Cell Line: J774A.1 murine macrophage cells
Concentration: 0-100 μM.
Incubation Time: 0.5 h (before LPS (1 μg/mL) treatment for 4.5 h).
Result: Inhibited the release of IL-1β in J774A.1 cells upon stimulation with LPS/ATP.
In Vivo

JC-171 treatment delays the progression and reduces the severity of experimental autoimmune encephalomyelitis (EAE) in mouse[1].

Animal Model: Mice immunized subcutaneously with 200 μg Myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide emulsified in Complete Freund’s Adjuvant (CFA) on day 0 followed by injection of 200 ng of pertussis toxin.
Dosage: 100 mg/kg, 10 mg/kg.
Administration: IP days 0, 1 and 2; and every other days thereafter (100 mg/kg).
Initiated when the clinical scores of individual mice have reached 1 (flaccid tail), and given every other day (10 mg/kg).
Result: Efficiently suppressed EAE progression compared with vehicle treatment.
Resulted in a substantial decrease in the frequency of MOG35–55-specific Th17 cells in the spleens and spinal cords of EAE mice.
Molecular Weight

384.83

Formula

C₁₆H₁₇ClN₂O₅S

CAS No.

2112809-98-8

SMILES

O=C(NCCC1=CC=C(S(=O)(NO)=O)C=C1)C2=CC(Cl)=CC=C2OC

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Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

JC-171JC171JC 171NOD-like Receptor (NLR)MultipleSclerosisEAEmacrophageinflammasomeInhibitorinhibitorinhibit

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