Biotin-16-dUTP
Biotin-16-dUTP (Biotin-16-deoxyuridine-5'-triphosphate) can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP can be used to produce biotinylated DNA probes in a variety of assay applications.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 86303-26-6
- 分子式: C32H52N7O18P3S
- 分子量:947.78
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
Biotin-16-dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 86303-26-6
-
分子量 947.78
-
分子式 C32H52N7O18P3S
-
SMILES
O[C@H]1C[C@H](N(C=C2/C=C/CNC(CCCCCNC(CCCNC(CCCC[C@@H]3SC[C@@]4(NC(N[C@@]43[H])=O)[H])=O)=O)=O)C(NC2=O)=O)O[C@@H]1COP(O)(OP(O)(OP(O)(O)=O)=O)=O
-
別名
Biotin-16-deoxyuridine-5'-triphosphate
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4. [Content Brief]
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)