Ubiquitination-IN-1
Based on 1 Customer Validation
Ubiquitination-IN-1 (compound 24) is a ubiquitination and Cksl-Skp2 protein-protein interaction (IC50=0.17 μM) inhibitor. Ubiquitination-IN-1 increases levels of p27. Ubiquitination-IN-1 has the potential for treatment disease by blocking the degradation of tumor suppressors.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.43%
- CAS 番号: 1819330-15-8
- 分子式: C21H14F3N3O2S
- 分子量:429.41
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Oxygen Sensing Compound Library
HY-L045
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Ubiquitination Compound Library
HY-L050
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Anti-Liver Cancer Compound Library
HY-L101
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Protein-protein Interaction Inhibitor Library
HY-L109
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Heterocyclic Compound Library
HY-L138
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Metabolic Enzyme Compound Library
HY-L146
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Mitophagy Compound Library
HY-L180
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MCE Bioactive Compound Library
HY-L001V
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Amino Acid Metabolism Compound Library
HY-L189
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
生物活性
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | GI50 |
3.06 μM
Compound: 22d
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Antiproliferative activity against human A549 cells after 6 days
Antiproliferative activity against human A549 cells after 6 days
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[PMID: 26463131] |
| A549 | IC50 |
5.91 μM
Compound: 22d
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Antiproliferative activity against human A549 cells after 2 days
Antiproliferative activity against human A549 cells after 2 days
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[PMID: 26463131] |
| HT-1080 | GI50 |
1.32 μM
Compound: 22d
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Antiproliferative activity against human HT1080 cells after 6 days
Antiproliferative activity against human HT1080 cells after 6 days
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[PMID: 26463131] |
| NCI-H1299 | IC50 |
3.9 μM
Compound: 22d
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Antiproliferative activity against human H1299 cells after 2 days
Antiproliferative activity against human H1299 cells after 2 days
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[PMID: 26463131] |
Ubiquitination-IN-1 inhibits A549 and HT1080 cells with IC50s of 0.91 and 0.4 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1819330-15-8
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性状 Solid
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分子量 429.41
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分子式 C21H14F3N3O2S
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Color White to yellow
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SMILES
O=S(C1=CC=CC(C(F)(F)F)=C1)(NC2=C3N=CC=CC3=C(C4=CC=CN=C4)C=C2)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 83.33 mg/mL (194.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.84 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (275 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3288 mL | 11.6439 mL | 23.2878 mL | 58.2194 mL |
| 5 mM | 0.4658 mL | 2.3288 mL | 4.6576 mL | 11.6439 mL | |
| 10 mM | 0.2329 mL | 1.1644 mL | 2.3288 mL | 5.8219 mL | |
| 15 mM | 0.1553 mL | 0.7763 mL | 1.5525 mL | 3.8813 mL | |
| 20 mM | 0.1164 mL | 0.5822 mL | 1.1644 mL | 2.9110 mL | |
| 25 mM | 0.0932 mL | 0.4658 mL | 0.9315 mL | 2.3288 mL | |
| 30 mM | 0.0776 mL | 0.3881 mL | 0.7763 mL | 1.9406 mL | |
| 40 mM | 0.0582 mL | 0.2911 mL | 0.5822 mL | 1.4555 mL | |
| 50 mM | 0.0466 mL | 0.2329 mL | 0.4658 mL | 1.1644 mL | |
| 60 mM | 0.0388 mL | 0.1941 mL | 0.3881 mL | 0.9703 mL | |
| 80 mM | 0.0291 mL | 0.1455 mL | 0.2911 mL | 0.7277 mL | |
| 100 mM | 0.0233 mL | 0.1164 mL | 0.2329 mL | 0.5822 mL |
Ubiquitination-IN-1 関連分類
HY-135199 関連分類
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