2. Others
  3. Others
  4. JY-2

JY-2 is a moderately selective and orally active Forkhead transcription factor forkhead box O1 (FoxO1) inhibitor that inhibits FoxO1 transcriptional activity with an IC50 of 22 μM. JY-2 shows moderate inhibition against FoxO3a and FoxO4. JY-2 shows anti-diabetic activity.

For research use only. We do not sell to patients.

JY-2 Chemical Structure

JY-2 Chemical Structure

CAS No. : 339103-05-8

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 60 In-stock
10 mg USD 100 In-stock
25 mg USD 200 In-stock
50 mg USD 300 In-stock
100 mg USD 450 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

JY-2 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

JY-2 is a moderately selective and orally active Forkhead transcription factor forkhead box O1 (FoxO1) inhibitor that inhibits FoxO1 transcriptional activity with an IC50 of 22 μM. JY-2 shows moderate inhibition against FoxO3a and FoxO4. JY-2 shows anti-diabetic activity[1].

IC50 & Target

22 μM (FoxO1 transcriptional activity)[1]
In Vitro

JY-2 (10-100 μM; 24 h) reduces palmitic acid (PA; HY-N0830)-induced lipotoxicity in HepG2 and INS-1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JY-2 Related Antibodies

Real Time qPCR[1]

Cell Line: HepG2 and INS-1 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 24 h
Result: Reduced palmitic acid (PA)-induced G6Pase and PEPCK mRNA expression. Inhibited PA-induced lipid accumulation. Reduced PA-induced mRNA expression of ER stress markers (ATF3, CHOP and GRP78).

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 4 h; in the presence of PA (500μM)
Result: Increased p-FoxO1 levels in the whole cell lysate with a concurrent reduction in nuclear FoxO1 levels.
In Vivo

JY-2 (50-200 mg/kg; oral; 3 times for two days or daily for 4 weeks) shows anti-diabetic effects in mice[1].
Pharmacokinetic parameters of JY-2[1]

Parameters i.v. (20 mg/kg) p.o. (50 mg/kg)
AUCall (ng·h/mL) 5017 ± 1038 12270 ± 2775
AUCinf.obs (ng·h/mL) 5030 ± 1037 12400 ± 2753
Cmax (ng/mL) 10790 ± 3269 6826 ± 2342
Tmax (h) 0.1 ± 0.1 0.8 ± 0.7
T1/2 (h) 0.8 ± 0.2 1.3 ± 0.4
MRTinf.obs (h) 0.7 ± 0.1 2.0 ± 0.1
F (%) 97.8

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice[1]
Dosage: 50, 100, 200 mg/kg
Administration: Oral, three times for two days (9:00 AM, 7:00 PM, 9:00 AM on the next day)
Result: Improved glucose tolerance. Significantly reduced the expression of G6Pase and PEPCK mRNA in the liver. Enhanced mRNA expression of insulin and PDX-1 in the pancreas.
Animal Model: db/db mice and C57BL/6J mice, high fat-diet-induced obese diabetic (DIO) model[1]
Dosage: 50, 100 mg/kg
Administration: Oral, once daily for 4 weeks
Result: Decreased the levels of fasting blood glucose, improved glucose tolerance. The expression of ColIV, a fibrosis marker, was also lowered.
Animal Model: C57BL/6J mice[1]
Dosage: 20 mg/kg or 50 mg/kg
Administration: IV or PO (Pharmacokinetic Analysis)
Result: Showed an overall good pharmacokinetic profile.
Molecular Weight

292.12

Formula

C13H7Cl2N3O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC(Cl)=C(C2=NC(C3=CC=CC=N3)=NO2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (342.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4233 mL 17.1163 mL 34.2325 mL
5 mM 0.6847 mL 3.4233 mL 6.8465 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4233 mL 17.1163 mL 34.2325 mL 85.5813 mL
5 mM 0.6847 mL 3.4233 mL 6.8465 mL 17.1163 mL
10 mM 0.3423 mL 1.7116 mL 3.4233 mL 8.5581 mL
15 mM 0.2282 mL 1.1411 mL 2.2822 mL 5.7054 mL
20 mM 0.1712 mL 0.8558 mL 1.7116 mL 4.2791 mL
25 mM 0.1369 mL 0.6847 mL 1.3693 mL 3.4233 mL
30 mM 0.1141 mL 0.5705 mL 1.1411 mL 2.8527 mL
40 mM 0.0856 mL 0.4279 mL 0.8558 mL 2.1395 mL
50 mM 0.0685 mL 0.3423 mL 0.6847 mL 1.7116 mL
60 mM 0.0571 mL 0.2853 mL 0.5705 mL 1.4264 mL
80 mM 0.0428 mL 0.2140 mL 0.4279 mL 1.0698 mL
100 mM 0.0342 mL 0.1712 mL 0.3423 mL 0.8558 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

JY-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JY-2339103-05-8JY2JY 2OtherslipotoxicityHepG2INS-1ColIVG6PasePEPCKATF3CHOPGRP78Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
JY-2
Cat. No.:
HY-153347
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.