FSBA hydrochloride
Based on 1 Customer Validation
FSBA (5'-p-Fluorosulfonylbenzoyladenosine) hydrochloride is a covalent modifier and affinity labeling reagent for adenine nucleotide-binding proteins. FSBA hydrochloride covalently attaches to the nucleotide-binding sites of pyruvate kinase, glutamate dehydrogenase, and p56lck, and to a lysine residue in the ATP-binding site of cAMP-dependent protein kinase, causing loss of enzymatic activity. FSBA hydrochloride can be used for the research of T lymphoma.
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- Purity: 99.06%
- CAS No.: 78859-42-4
- 화학식: C17H17ClFN5O7S
- 분자량:489.86
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보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
FSBA (2.5 mM; 2 h) hydrochloride covalently modifies bovine liver glutamate dehydrogenase at a stoichiometry of 1.4 mol FSBA per mol enzyme, and the modified protein is specifically detected by affinity-purified anti-FSBA antibodies on immunoblots[1].
FSBA (2.5 mM; 2 h) hydrochloride covalently modifies rabbit muscle pyruvate kinase, and the modified protein is specifically detected by affinity-purified anti-FSBA antibodies on immunoblots[1].
FSBA (0.9 mM; 0-40 min) hydrochloride covalently modifies the bovine heart catalytic subunit of cAMP-dependent protein kinase in a time-dependent manner, resulting in proportional loss of kinase activity, with modified enzyme specifically detected by affinity-purified anti-FSBA antibodies[1].
FSBA (0.9 mM; 30 min) hydrochloride covalently modifies multiple nucleotide-binding proteins in cytosolic fractions from murine splenocytes, with labeling blocked by competing MgATP, and only minimally affected by increasing Mn2+ concentrations[1].
FSBA (0.125 mM; 25 min) hydrochloride covalently modifies the p56lck protein-tyrosine kinase in postnuclear particulate fractions from LSTRA cells, with far greater labeling observed in p56lck-overexpressing LSTRA cells than in SL-2 cells, and labeling blocked by competing MgATP[1].
FSBA (10-400 μM; 20 min) hydrochloride binds in a saturable manner to the p56lck protein-tyrosine kinase in postnuclear particulate fractions from LSTRA cells, confirming specific interaction with its nucleotide-binding site[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 78859-42-4
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Appearance Solid
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분자량 489.86
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화학식 C17H17ClFN5O7S
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Color White to off-white
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SMILES
NC1=C(C2=NC=N1)N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)COC(C4=CC=C(C=C4)S(=O)(F)=O)=O)O)O.Cl
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Synonyms
5'-p-Fluorosulfonylbenzoyladenosine
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 125 mg/mL (255.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (277 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Anostario M Jr, et al. Immunochemical detection of adenine nucleotide-binding proteins with antibodies to 5'-p-fluorosulfonylbenzoyladenosine. Anal Biochem. 1990 Oct;190(1):60-5. [Content Brief]
[2]. Ramaswamy S, et al. Nucleotide binding to the C-terminal nucleotide binding domain of ArsA. Studies with an ATP analogue, 5'-p-fluorosulfonylbenzoyladenosine. J Biol Chem. 1998 Apr 10;273(15):9243-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0414 mL | 10.2070 mL | 20.4140 mL | 51.0350 mL |
| 5 mM | 0.4083 mL | 2.0414 mL | 4.0828 mL | 10.2070 mL | |
| 10 mM | 0.2041 mL | 1.0207 mL | 2.0414 mL | 5.1035 mL | |
| 15 mM | 0.1361 mL | 0.6805 mL | 1.3609 mL | 3.4023 mL | |
| 20 mM | 0.1021 mL | 0.5103 mL | 1.0207 mL | 2.5517 mL | |
| 25 mM | 0.0817 mL | 0.4083 mL | 0.8166 mL | 2.0414 mL | |
| 30 mM | 0.0680 mL | 0.3402 mL | 0.6805 mL | 1.7012 mL | |
| 40 mM | 0.0510 mL | 0.2552 mL | 0.5103 mL | 1.2759 mL | |
| 50 mM | 0.0408 mL | 0.2041 mL | 0.4083 mL | 1.0207 mL | |
| 60 mM | 0.0340 mL | 0.1701 mL | 0.3402 mL | 0.8506 mL | |
| 80 mM | 0.0255 mL | 0.1276 mL | 0.2552 mL | 0.6379 mL | |
| 100 mM | 0.0204 mL | 0.1021 mL | 0.2041 mL | 0.5103 mL |
FSBA hydrochloride Related Classifications
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