2. Lalistat 2

Lalistat 2 is an inhibitor of many lipases especially Lysosomal acid lipase (LAL, IC50 = 152 nM), which is a key enzyme that degrades neutral lipids at an acidic pH in lysosomes. Lalistat 2 is commonly used to investigate the cell-specific functions of LAL and LAL deficiency in vitro, as well as specifically measure LAL activity in human blood samples or cells .

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Lalistat 2 Chemical Structure

Lalistat 2 Chemical Structure

CAS No. : 1234569-09-5

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Solid + Solvent
10 mM * 1 mL in DMSO
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 USD 121 In-stock
Solution
10 mM * 1 mL in DMSO USD 121 In-stock
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10 mg USD 170 In-stock
50 mg USD 620 In-stock
100 mg USD 980 In-stock
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Based on 1 publication(s) in Google Scholar

Lalistat 2 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Lalistat 2 is an inhibitor of many lipases especially Lysosomal acid lipase (LAL, IC50 = 152 nM), which is a key enzyme that degrades neutral lipids at an acidic pH in lysosomes. Lalistat 2 is commonly used to investigate the cell-specific functions of LAL and LAL deficiency in vitro, as well as specifically measure LAL activity in human blood samples or cells [1][2][3].

IC50 & Target

IC50: 152 nM (LAL)[2]
In Vitro

Lalistat 2 (30 μM, 7 d) does not affect differentiation of mice adipocytes but reduces isoproterenol-stimulated lipolysis[1].
Lalistat 2 (30 μM, 7 d) inhibits neutral lipid hydrolase activity in mice adipocytes[1].
Lalistat 2 (0.1-100 μM, 20 h) inhibits neutral lipid hydrolases in bone marrow-derived macrophages (BMDM) in a dose-dependent manner[1].
Lalistat 2 (30 μM, 24 h) inhibits adipose triglyceride lipase and hormone-sensitive lipase in mouse and human COS-7 cells[1].
Lalistat 2 (30 μM, 20 h) decreases activities of LAL， ATGL， HSL， MGL， PNPLA6 and NCEH1 in mice bone marrow-derived macrophages[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lalistat 2 Related Antibodies
Molecular Weight

296.39

Formula

C13H20N4O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCCCC1)OC2=NSN=C2N3CCCCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (337.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3739 mL 16.8697 mL 33.7393 mL
5 mM 0.6748 mL 3.3739 mL 6.7479 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (253 KB) HNMR (246 KB) LCMS (261 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3739 mL 16.8697 mL 33.7393 mL 84.3483 mL
5 mM 0.6748 mL 3.3739 mL 6.7479 mL 16.8697 mL
10 mM 0.3374 mL 1.6870 mL 3.3739 mL 8.4348 mL
15 mM 0.2249 mL 1.1246 mL 2.2493 mL 5.6232 mL
20 mM 0.1687 mL 0.8435 mL 1.6870 mL 4.2174 mL
25 mM 0.1350 mL 0.6748 mL 1.3496 mL 3.3739 mL
30 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8116 mL
40 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1087 mL
50 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6870 mL
60 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4058 mL
80 mM 0.0422 mL 0.2109 mL 0.4217 mL 1.0544 mL
100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lalistat 21234569-09-5Lalistat2Lalistat-2dried blood spotLAL inhibitorLAL deficiencyWolman diseaseCE storage diseaseatLal-/- miceLal-/- miceInhibitorinhibitorinhibit

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