1. Apoptosis
  2. Apoptosis
  3. M50054

M50054 

Cat. No.: HY-103347
Handling Instructions

M50054 is a potent inhibitor of apoptosis. M50054 inhibits Etoposide-induced caspase-3 activation of U937 cells with an IC50 of 79 μg/mL. M50054 does not directly inhibit the enzymatic activity of caspase-3. M50054 can be used for the research anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia.

For research use only. We do not sell to patients.

M50054 Chemical Structure

M50054 Chemical Structure

CAS No. : 54135-60-3

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Description

M50054 is a potent inhibitor of apoptosis. M50054 inhibits Etoposide-induced caspase-3 activation of U937 cells with an IC50 of 79 μg/mL. M50054 does not directly inhibit the enzymatic activity of caspase-3. M50054 can be used for the research anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia[1].

In Vitro

M50054 inhibits the caspase-3 activation by Etoposide in a dose-dependent manner. The IC50 value of M50054 against Etoposide-induced caspase-3 activation of U937 cells is 79 μg/mL. However, M50054 does not directly inhibit the enzymatic activity of caspase-3 at all concentrations up to 1000 μg/mL[1].
M50054 (1-300 μg/mL) inhibits soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL[1].
M50054 (30-300 μg/mL) inhibits the death of U937 cells induced by etoposide (10 μg/mL) in a dose-dependent manner, and the IC50 value of M50054 against Etoposide-induced cell death of U937 cells is 130 μg/mL. The IC50 value of M50054 against etoposide-induced DNA fragmentation of U937 cells was 54 μg/mL[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Human Fas-expressing WC8 cells
Concentration: 1, 3, 10, 30, 100, 300 μg/mL
Incubation Time: Pre-incubated for 30 min
Result: Inhibited soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL.
In Vivo

M50054 (10-300 mg/kg; oral administration 30 min before anti-Fas antibody injection) inhibits the elevation of alanine aminotransferase and aspartate aminotransferase in a dose-dependent manner[1].
M50054 (topical treatment; given daily beginning on day 5 after birth and ending on day 14) significantly improves alopecia (hair loss) symptoms[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice[1]
Dosage: 10, 30, 100 or 300 mg/kg
Administration: Orally administered in a volume of 10 mL/kg
Result: Inhibited the elevation of alanine aminotransferase and aspartate aminotransferase in a dose-dependent manner.
Significantly improved these parameters at doses of 100 mg/kg and 300 mg/kg.
Animal Model: Five-day-old SD rats[1]
Dosage:
Administration: Topically applied to the head and back of 5-day-old rats once daily for 10 days
Result: Alopecia scores significantly decreased throughout the entire observation period compared with the control group.
Molecular Weight

236.26

Formula

C13H16O4

CAS No.
SMILES

O=C1C(CC(C(CCC2)=O)C2=O)C(CCC1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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M50054
Cat. No.:
HY-103347
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