2. Immunology/Inflammation Anti-infection
  3. Nuclear Factor of activated T Cells (NFAT) Bacterial
  4. Maltobionic acid

Maltobionic acid  (Synonyms: 4-O-α-D-Glucopyranosyl-D-gluconic acid)

Cat. No.: HY-W423191 Purity: 90.0%
COA
SDS
Handling Instructions Technical Support

Maltobionic acid (4-O-α-D-Glucopyranosyl-D-gluconic acid) is an orally active oligosaccharide and iron chelator with antibacterial activity. Maltobionic acid inhibits the expression of NFATc1, suppresses osteoclast differentiation, inhibits bone resorption, and increases serum calcitonin levels. Maltobionic acid protects mammalian cells from hydrogen peroxide (H2O2)-induced oxidative damage; resists fermentation by the gut microbiota; and exhibits anti-digestive and anti-fermentative properties. Maltobionic acid can be used in research related to osteoporosis, bacterial infections, and constipation.

For research use only. We do not sell to patients.

Maltobionic acid

Maltobionic acid Chemical Structure

CAS No. : 534-42-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Maltobionic acid Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Maltobionic acid (4-O-α-D-Glucopyranosyl-D-gluconic acid) is an orally active oligosaccharide and iron chelator with antibacterial activity. Maltobionic acid inhibits the expression of NFATc1, suppresses osteoclast differentiation, inhibits bone resorption, and increases serum calcitonin levels. Maltobionic acid protects mammalian cells from hydrogen peroxide (H2O2)-induced oxidative damage; resists fermentation by the gut microbiota; and exhibits anti-digestive and anti-fermentative properties. Maltobionic acid can be used in research related to osteoporosis, bacterial infections, and constipation[1][2][3][4].

In Vitro

Maltobionic acid (0.01-1000 μM; 72 h) significantly inhibits sRANKL (an NF-κB activator)-induced osteoclast differentiation of RAW264 cells without reducing cell viability[1].
Maltobionic acid (100-1000 μM; 48 h) significantly reduces the expression of NFATc1, a key transcription factor for osteoclast differentiation, in sRANKL-stimulated RAW264 cells[1].
Maltobionic acid (100-1000 μM; 15 min) significantly enhances sRANKL-induced IκBα phosphorylation in RAW264 cells[1].
Maltobionic acid (0.4-50 mg/mL; 24 h) inhibits the growth of *Salmonella choleraesuis*, *Escherichia coli*, *Staphylococcus aureus* and *Listeria monocytogenes*, with MIC values of 8.0, 8.5, 10.5 and 8.0 mg/mL, respectively[2].
Maltobionic acid (100 μg/mL; 24.5 h) exerts cytoprotective effects on HEK-293 cells against H2O2-induced oxidative damage[2].
Cassava starch-chitosan films supplemented with 7.5% (w/w) maltose acid inhibit the growth of *Listeria monocytogenes*, *Salmonella choleraesuis*, *Escherichia coli* and *Staphylococcus aureus*, with a zone of inhibition diameter ranging from 15.67 to 22.33 mm[2].
Sodium maltobionate (10% (w/v) to 1% (w/w); 30 min-6 h) is completely indigestible by artificial saliva, gastric juice, and pancreatic juice in vitro, and is only minimally (3.3%) digested by rat intestinal enzymes[4].
Sodium maltobionate (0.5% (w/v); 3 days) is selectively utilized by specific human intestinal bacteria, and exhibits strong proliferative activity against Bifidobacterium dentium and Bifidobacterium adolescentis[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Maltobionic acid Related Antibodies

Cell Differentiation Assay[1]

Cell Line: murine macrophage RAW264 cells
Concentration: 0.01, 0.1, 1, 10, 100 and 1000 μM
Incubation Time: 72 h
Result: Caused a significant decrease in the number of multinucleated (4+ nuclei) tartrate-resistant acid phosphatase-positive osteoclast-like cells relative to control.
Did not significantly affect cell viability at any tested concentration.

Western Blot Analysis[1]

Cell Line: murine macrophage RAW264 cells
Concentration: 0.01, 0.1, 1, 10, 100 and 1000 μM
Incubation Time: 48 h
Result: Caused a significant decrease in NFATc1 protein expression relative to the sRANKL-treated control.

Western Blot Analysis[1]

Cell Line: murine macrophage RAW264 cells
Concentration: 0.01, 0.1, 1, 10, 100 and 1000 μM
Incubation Time: 15 min
Result: Caused a significant increase in phosphorylated IκBα protein levels relative to the sRANKL-treated control.
In Vivo

Maltobionic acid (5.0% (w/w); administered via mixed feed for oral ingestion; ad libitum access; 80 days) improves bone mineral density and bone metabolism in ovariectomized mice by significantly increasing femoral mineral content and serum calcitonin levels, and reducing serum TRACP-5b levels, a marker of bone resorption[1].
Maltobionic acid (1600 mg/kg; p.o.; single administration) exhibits high resistance to digestion and fermentation in rats[3].
Maltobionic acid enhances intestinal absorption of Ca2+ and Mg2+ in healthy rats, and increases the weight of cecal contents and levels of short-chain fatty acids[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ddY mice (4-week-old female; ovariectomized)[1]
Dosage: 5.0% (w/w)
Administration: p.o.; ad libitum; 80 days
Result: Increased femur dry weight to 60.8 mg (vs. 52.4 mg in control).\nIncreased femur calcium content to 12.7 mg (vs. 10.2 mg in control).
Increased femur phosphorus content to 5.29 mg (vs. 4.12 mg in control).
Increased serum calcitonin levels significantly.
Decreased serum tartrate-resistant acid phosphatase 5b (TRACP-5b) levels significantly.
Showed an upward trend in femoral bone mineral density.
Animal Model: Wistar rats (male, initial body weight 220 g)[3]
Dosage: 1600 mg/kg
Administration: p.o.; single dose
Result: Was minimally hydrolyzed by brush border membrane vesicles (BBMV).
Produced no significant difference in exhaled hydrogen levels over 8 h following administration compared with controls.
Molecular Weight

358.30

Formula

C12H22O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@@H]1O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (279.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7910 mL 13.9548 mL 27.9096 mL
5 mM 0.5582 mL 2.7910 mL 5.5819 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.86%

SDS (393 KB)

COA (253 KB) HNMR (134 KB) RP-HPLC (243 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7910 mL 13.9548 mL 27.9096 mL 69.7739 mL
5 mM 0.5582 mL 2.7910 mL 5.5819 mL 13.9548 mL
10 mM 0.2791 mL 1.3955 mL 2.7910 mL 6.9774 mL
15 mM 0.1861 mL 0.9303 mL 1.8606 mL 4.6516 mL
20 mM 0.1395 mL 0.6977 mL 1.3955 mL 3.4887 mL
25 mM 0.1116 mL 0.5582 mL 1.1164 mL 2.7910 mL
30 mM 0.0930 mL 0.4652 mL 0.9303 mL 2.3258 mL
40 mM 0.0698 mL 0.3489 mL 0.6977 mL 1.7443 mL
50 mM 0.0558 mL 0.2791 mL 0.5582 mL 1.3955 mL
60 mM 0.0465 mL 0.2326 mL 0.4652 mL 1.1629 mL
80 mM 0.0349 mL 0.1744 mL 0.3489 mL 0.8722 mL
100 mM 0.0279 mL 0.1395 mL 0.2791 mL 0.6977 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Maltobionic acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Maltobionic acid534-42-94-O-α-D-Glucopyranosyl-D-gluconic acidNuclear Factor of activated T Cells (NFAT)Bacterialnuclear factor of activated T-cell cytoplasmic 1bone resorptionosteoclast differentiationovariectomy-induced femoral bone mineral contentHEK-293 cellsSalmonella enterica serovar CholeraesuisEscherichia coliRAW264 cellsserum calcitoninovariectomy-induced femoral bone mineral densityInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Maltobionic acid
Cat. No.:
HY-W423191
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.