2. Apoptosis
  3. Apoptosis
  4. Millepachine

Millepachine 

Cat. No.: HY-N7591 Purity: 99.65%
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Millepachine is a bioactive natural chalcone from Chinese herbal medicine Millettia pachycarpa Benth, exhibits strong antitumor effects against numerous human cancer cells both in vitro and in vivo.

For research use only. We do not sell to patients.

Millepachine Chemical Structure

Millepachine Chemical Structure

CAS No. : 1393922-01-4

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Solution
10 mM * 1 mL in DMSO USD 275 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 275 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 250 In-stock
Estimated Time of Arrival: December 31
10 mg USD 400 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1400 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2240 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

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Description

Millepachine is a bioactive natural chalcone from Chinese herbal medicine Millettia pachycarpa Benth, exhibits strong antitumor effects against numerous human cancer cells both in vitro and in vivo[1].

In Vitro

Millepachine (1.25-20 μM; 48 h) remarkably inhibits the proliferation of cisplatin-resistant A2780CP cells[1].
Millepachine (2-8 μM; 24 or 48 h) induces G2/M arrest and apoptosis cisplatin-sensitive A2780S and cisplatin-resistant A2780CP cells[1].
Millepachine (2-8 μM; 24 h) decreases topoisomerase II levels in A2780S and A2780CP cells[1].
Millepachine (2-8 μM; 24 h) inhibits ATP-binding cassette transporter activity in A2780CP cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: A2780CP cells
Concentration: 0, 1.25, 2.5, 5, 10, 20 μM
Incubation Time: 48 hours
Result: Inhibited the cells proliferation with an IC50 of 4 μM.

Cell Cycle Analysis[1]

Cell Line: A2780S and A2780CP cells
Concentration: 2, 4, 8 μM
Incubation Time: 24 or 48 hours
Result: Induced significant G2/M arrest both in both cells.
The percentage of cells in the G2/M fraction increased from 15.99% in vehicle cells to 24.93%, 60.67%, and 77.31% at dose of 2, 4, and 8 μM in A2780S cells, respectively.

Apoptosis Analysis[1]

Cell Line: A2780S and A2780CP cells
Concentration: 2, 4, 8 μM
Incubation Time: 24 or 48 hours
Result: The percentage of apoptotic cells increased from 1.49% in vehicle cells to 10.98%, 20.60%, and 39.43% at dose of 2, 4, and 8 μM in A2780S cells, respectively.
The percentage of apoptotic cells increased from 0.87% to 10.97%, 25.28%, and 37.59% in A2780CP cells, respectively.

Western Blot Analysis[1]

Cell Line: A2780S and A2780CP cells
Concentration: 0, 2, 4, 8 μM
Incubation Time: 24 hours
Result: Decreased the levels of topoisomerase II (TOPO II) in both cells.
In Vivo

Millepachine (20 mg/kg; i.v. every two days for 14 day) inhibits tumor growth in mice[1].
Millepachine (20 mg/kg; i.v. every two days for 14 day) does not induce acquired drug resistance in an excised A2780S xenograft model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells[1]
Dosage: 20 mg/kg
Administration: I.v. every two days for 2-14 days
Result: Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively.
With low toxicity in vivo.
Molecular Weight

350.41

Appearance

Solid

Formula

C22H22O4
CAS No.
SMILES

O=C(C1=C2C(C=CC(C)(C)O2)=C(OC)C=C1)/C=C/C3=CC=C(OC)C=C3
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (285.38 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8538 mL 14.2690 mL 28.5380 mL
5 mM 0.5708 mL 2.8538 mL 5.7076 mL
10 mM 0.2854 mL 1.4269 mL 2.8538 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (7.13 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Millepachine1393922-01-4ApoptosisbioactivenaturalchalconeChineseherbalmedicineMillettiapachycarpaBenthantitumorcancerInhibitorinhibitorinhibit

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