1. Cell Cycle/DNA Damage Cytoskeleton
  2. MASTL
  3. MKI-1

MKI-1  (Synonyms: MASTL Kinase Inhibitor-1)

Cat. No.: HY-137552 Purity: 98.13%
COA Handling Instructions

MKI-1, an inhibitor of MASTL (microtubule-associated serine/threonine kinase-like) with an IC50 of 9.9 μM, exerts antitumor and radiosensitizer activities through PP2A activation in breast cancer.

For research use only. We do not sell to patients.

MKI-1 Chemical Structure

MKI-1 Chemical Structure

CAS No. : 1190277-80-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 264 In-stock
Solution
10 mM * 1 mL in DMSO USD 264 In-stock
Solid
5 mg USD 240 In-stock
10 mg USD 385 In-stock
25 mg USD 650 In-stock
50 mg USD 1050 In-stock
100 mg USD 1680 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

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Description

MKI-1, an inhibitor of MASTL (microtubule-associated serine/threonine kinase-like) with an IC50 of 9.9 μM, exerts antitumor and radiosensitizer activities through PP2A activation in breast cancer[1].

In Vitro

MKI-1 (5-20 μM) inhibits the activity of MASTL in breast cancer cells[1].
MKI-1 (100 μM, 72 h) inhibits various oncogenic properties of breast cancer cells but showed much weaker effects on the viability of normal breast cells[1].
MKI-1 clearly reduces both serine 62-phosphorylation of c-Myc and total c-Myc, with a decrease in ENSA phosphorylation[1].
MKI-1 (20 μM, 16 h) reduces c-Myc stability through PP2A activation in MCF7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: MCF7 and T47D cells.
Concentration: 5-20 μM.
Incubation Time: 24 h.
Result: Inhibited the phosphorylation of ENSA in MCF7 and T47D cells.
Significantly inhibited the phosphorylation of ENSA in mitotic cells.
In Vivo

MKI-1 (50 mg/kg, ip, twice a week) reduces tumor growth and enhances the radiosensitivity of BT549 xenograft model in response to 6 Gy irradiation compared with the control group, with no notable changes in body weight, suggesting the absence of gross toxicity in the treated mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Five-week-old female BALB/c nude mice (BT549 cells)[1].
Dosage: 50 mg/kg.
Administration: Twice per week by intraperitoneal (i.p.) injection.
Result: Reduced tumor growth.
Molecular Weight

302.33

Formula

C18H14N4O

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NC1=NC2=CC=CC=C2N1)C3=CC=CC(N4C=CC=C4)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (826.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3076 mL 16.5382 mL 33.0764 mL
5 mM 0.6615 mL 3.3076 mL 6.6153 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3076 mL 16.5382 mL 33.0764 mL 82.6911 mL
5 mM 0.6615 mL 3.3076 mL 6.6153 mL 16.5382 mL
10 mM 0.3308 mL 1.6538 mL 3.3076 mL 8.2691 mL
15 mM 0.2205 mL 1.1025 mL 2.2051 mL 5.5127 mL
20 mM 0.1654 mL 0.8269 mL 1.6538 mL 4.1346 mL
25 mM 0.1323 mL 0.6615 mL 1.3231 mL 3.3076 mL
30 mM 0.1103 mL 0.5513 mL 1.1025 mL 2.7564 mL
40 mM 0.0827 mL 0.4135 mL 0.8269 mL 2.0673 mL
50 mM 0.0662 mL 0.3308 mL 0.6615 mL 1.6538 mL
60 mM 0.0551 mL 0.2756 mL 0.5513 mL 1.3782 mL
80 mM 0.0413 mL 0.2067 mL 0.4135 mL 1.0336 mL
100 mM 0.0331 mL 0.1654 mL 0.3308 mL 0.8269 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MKI-1
Cat. No.:
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