N-Acetylneuraminate lyase (CgNal)
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N-Acetylneuraminate lyase (CgNal) (Sialic acid aldolase (CgNal)) is a rate-limiting enzyme in bacteria metabolism that catalyzes the reversible aldol cleavage of sialic acid, and it regulates the overall metabolism of the bacterial sialic acid catabolic pathway. N-Acetylneuraminate lyase (CgNal) catalyzes the reversible condensation reaction of pyruvate and N-acetyl-d-mannosamine (ManNAc) to produce sialic acid N-acetylneuraminic acid (Neu5Ac). The protein expression of N-Acetylneuraminate lyase is downregulated at the translational level under the regulation of methyl methanesulfonate, and this change significantly impairs the adhesion and invasion abilities of bacteria to eukaryotic cells. N-Acetylneuraminate lyase is a key virulence-related protein in various pathogenic bacteria and is considered an antibiotic drug target. N-Acetylneuraminate lyase (CgNal) can be used in studies of invasive (extraintestinal) infectious diseases, Crohn's disease, and methicillin-resistant Staphylococcus aureus infections.
For research use only. We do not sell to patients.
- CAS No.: 9027-60-5
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Purified MRSA N-Acetylneuraminate lyase (CgNal) (0.16, 40 mM sialic acid) exhibits a hyperbolic substrate response to sialic acid with a Km of 3.2 mM, a Vmax of 40.0 μmol min-1 mg-1, and a kcat of 22.1 s-1 at 30 °C[3].
Purified MRSA N-Acetylneuraminate lyase (CgNal) (0.16, 20 mM sialic acid; 0, 0.16, 0.31, 0.63, 1.25, 2.5, 5 mM sialic acid alditols) is competitively inhibited by a diastereomeric mixture of sialic acid alditols with a Ki of 0.39 mM at 37 °C[3].
Purified MRSA N-Acetylneuraminate lyase (CgNal) (5, 10, 15 μM) exists as a stable tetrameric species in solution across concentrations of 5, 10, and 15 μM, with a sedimentation coefficient of 6.7 S and an apparent molecular mass of 123 kDa at 20 °C[3].
MRSA N-Acetylneuraminate lyase (CgNal) (5 mM sialic acid alditols) forms a stable complex with the 2R-diastereoisomer of sialic acid alditol, with the inhibitor bound in the enzyme's active site via multiple hydrogen bonds, as shown by a 2.33 Å resolution crystal structure[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9027-60-5
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[N-Acetylneuraminate lyase (CgNal)]
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Synonyms
Sialic acid aldolase (CgNal)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Vimr ER, et al. Diversity of microbial sialic acid metabolism. Microbiology and molecular biology reviews : MMBR. 2004 Mar;68(1):132-53. [Content Brief]
[2]. Di Pasquale P, et al. Exposure of E. coli to DNA-Methylating Agents Impairs Biofilm Formation and Invasion of Eukaryotic Cells via Down Regulation of the N-Acetylneuraminate Lyase NanA. Frontiers in microbiology. 2016;7:147. [Content Brief]
[3]. North RA, et al. Structure and inhibition of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus. FEBS letters. 2016 Dec;590(23):4414-4428. [Content Brief]
[4]. Li Y, et al. Pasteurella multocida sialic acid aldolase: a promising biocatalyst. Applied microbiology and biotechnology. 2008 Jul;79(6):963-70. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
N-Acetylneuraminate lyase (CgNal) Related Classifications
HY-P2977 Related Classifications
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- N-Acetylneuraminate lyase (CgNal)
- 9027-60-5
- Sialic acid aldolase (CgNal)
- Endogenous Metabolite
- Fructose-1,6-bisphosphate aldolase
- biofilm formation
- MRSA
- N-Acetylneuraminate lyase
- 5-O-methyl-ManNAc
- sialic acid
- E. coli
- sialic acid catabolism
- Staphylococcus aureus
- Crohn’s disease
- extraintestinal infectious diseases
- Inhibitor
- inhibitor
- inhibit