2. Protein Tyrosine Kinase/RTK
  3. Pyk2
  4. PF-4618433

PF-4618433 

Cat. No.: HY-18312 Purity: ≥98.0%
COA Handling Instructions

PF-4618433 is a potent and selective PYK2 inhibitor, with an IC50 of 637 nM. PF-4618433 may be suitable for the research of osteoporosis, craniofacial and appendicular skeletal defects and for targeted bone regeneration.

For research use only. We do not sell to patients.

PF-4618433 Chemical Structure

PF-4618433 Chemical Structure

CAS No. : 1166393-85-6

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 451 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 451 In-stock
Solid
5 mg USD 410 In-stock
10 mg   Get quote  
50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Description

PF-4618433 is a potent and selective PYK2 inhibitor, with an IC50 of 637 nM. PF-4618433 may be suitable for the research of osteoporosis, craniofacial and appendicular skeletal defects and for targeted bone regeneration[1][2].

IC50 & Target

IC50: 637 nM (PYK2)[1]
In Vitro

PF-4618433 (0.1-1.0 μM; 7 days) promotes osteogenesis of hMSC cultures. PF-4618433 increases in both alkaline phosphatase (ALP) activity and mineralization in a dependent manner[1].
PF-4618433 (0.1-0.3 μM; 24 hours) enhances osteoblast proliferation[2].
PF-4618433 (0.0125-0.3 μM; 14 or 21 days) enhances calcium deposition at the concentrations of 0.1 and 0.3 μM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: Murine bone marrow-derived mesenchymal stem cells (BMSC)
Concentration: 0.1, 0.3 μM
Incubation Time: 24 hours
Result: Increased cell proliferation activity significantly when compared to the untreated or control group.
Molecular Weight

445.52

Appearance

Solid

Formula

C24H27N7O2
CAS No.
SMILES

O=C(NC1=CC(COC2=CC=CN=C2)=NN1)NC3=CC(C(C)(C)C)=NN3C4=CC=C(C)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (280.57 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2446 mL 11.2228 mL 22.4457 mL
5 mM 0.4489 mL 2.2446 mL 4.4891 mL
10 mM 0.2245 mL 1.1223 mL 2.2446 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.62%

COA (252 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

PF-46184331166393-85-6PF4618433PF 4618433Pyk2Proline-rich tyrosine kinase 2PYK2osteoporosisBMSChMSCalkalinephosphataseALPInhibitorinhibitorinhibit

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