PNR-3-80 

PNR-3-80 is an endonuclease G (ENDOG) inhibitor with an IC₅₀ of 0.67 μM. As a non-competitive binder to the ENDOG-substrate complex, PNR-3-80 specifically inhibits the endonuclease activity of ENDOG. PNR-3-80 reduces cell death induced by Cisplatin (HY-17394) and Docetaxel (HY-B0011), and inhibits DNA damage and autophagy (autophagy) induced by Etoposide (HY-13629). PNR-3-80 can be used in studies related to cell injury^[1][2].

PNR-3-80 (50 μM; 24 h) inhibits endonuclease G (ENDOG)-dependent DNA damage and autophagy induction in human hepatocytes^[1].
PNR-3-80 potently inhibits recombinant mouse EndoG in high-throughput luciferase reporter gene assays, with an IC₅₀ of 0.67 μM^[2].
PNR-3-80 (1 μM for 1 h against mouse EndoG; 1 mM for 1 h against human EndoG) protects plasmid DNA from degradation by EndoG in plasmid nicking assays^[2].
PNR-3-80 (pre-incubated for 2 h followed by co-incubation with Cisplatin for 24 h at a concentration of 50 μM) significantly protects 22Rv1 human prostate cancer cells that endogenously express EndoG against Cisplatin-induced cell death^[2].
PNR-3-80 (preincubated for 2 h followed by co-incubation with docetaxel for 24 h at a concentration of 50 μM) significantly protects human prostate adenocarcinoma PC3 cells overexpressing EndoG from Docetaxel (HY-B0011)-induced cell death and DNA fragmentation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

459.90

C₂₄H₁₄ClN₃O₃S

1424353-63-8

Solid

Light yellow to yellow

O=C1NC(NC(/C1=C\C2=CN(C3=C2C=C(Cl)C=C3)C(C4=CC5=C(C=CC=C5)C=C4)=O)=O)=S

Room temperature in continental US; may vary elsewhere.

• [1]. Wang W, et al. Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Nat Commun. 2021;12(1):476. Published 2021 Jan 20.  [Content Brief]

  [2]. Jang DS, et al. Novel cytoprotective inhibitors for apoptotic endonuclease G. DNA Cell Biol. 2015;34(2):92-100.  [Content Brief]