PPADS tetrasodium

Name: PPADS tetrasodium
Brand: MedChemExpress
SKU: HY-101044
Rating: 5.0 (5 reviews)

Cat. No.: HY-101044 Purity: ≥95.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

PPADS tetrasodiuma is a non-selective P2X receptor antagonist. PPADS tetrasodiuma blocks recombinant P2X1, -2, -3, -5 with IC₅₀s ranging from 1 to 2.6 μM. PPADS tetrasodiuma blocks native P2Y2-like (IC₅₀~0.9 mM) and recombinant P2Y4 (IC₅₀~15 mM) receptors. PPADS tetrasodiuma is an inhibitor of the reverse mode of the Na/Ca²⁺ exchanger in guinea pig airway smooth muscle.

For research use only. We do not sell to patients.

PPADS tetrasodium Chemical Structure

CAS No. : 192575-19-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of PPADS tetrasodium:

Iso-PPADS tetrasodium Get quote
PPADS Get quote

5 Publications Citing Use of MCE PPADS tetrasodium

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P2X1 Receptor P2X2 Receptor P2X3 Receptor P2X4 Receptor P2X7 Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

p2x1 Receptor

In Vitro

PPADS tetrasodiuma (1-30 μM; 10-50 minutes) inhibits Na+/Ca2+ exchanger reverse mode (NCXREV) in a time- and concentration dependent manner^[2].
PPADS tetrasodiuma is effective at other native and recombinant P2XRs. At human P2XRs sensitivity to PPADS tetrasodiuma depended on the subtype and is highest at the hP2X1, -2, -3, -5, and -7Rs with an IC₅₀ of ~1-3 and ~30 μM for the hP2X4R^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PPADS tetrasodiuma (15-60 mg/100g body weight (BW); i.p.; every 12 hours for 8 days) inhibits glomerular mesangial cells (MC) proliferation without altering proliferation of non-MC in vivo in mesangial proliferative glomerulonephritis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley ratsweighing 160 to 200 g (anti-Thy1 disease mode)^[4]
Dosage:	15 mg/100g BW, 30 mg/100g BW, 60 mg/100g BW
Administration:	i.p.; every 12 hours for 8 days (the first PPADS injection was administered 60 minutes after disease induction, and the loading dose always contained double the amount of PPADS compared to the following injections.)
Result:	Specifically and dose-dependently reduced early (day 3) glomerular mesangial cell proliferation without altering proliferation of non-MC.

Molecular Weight

599.30

Formula

C₁₄H₁₀N₃Na₄O₁₂PS₂

CAS No.

192575-19-2

Appearance

Solid

Color

Orange to red

SMILES

O=S(C1=CC=C(/N=N/C2=NC(C)=C(O)C(C=O)=C2COP(O[Na])(O[Na])=O)C(S(=O)(O[Na])=O)=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (83.43 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6686 mL	8.3431 mL	16.6861 mL
5 mM	0.3337 mL	1.6686 mL	3.3372 mL
10 mM	0.1669 mL	0.8343 mL	1.6686 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥95.0%

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Data Sheet (282 KB) SDS (252 KB)

COA (258 KB)

Handling Instructions (2659 KB)

References

[1]. Flores-Soto E, et al. PPADS, a P2X receptor antagonist, as a novel inhibitor of the reverse mode of the Na⁺/Ca²⁺ exchanger in guinea pig airway smooth muscle. Eur J Pharmacol. 2012 Jan 15;674(2-3):439-44. [Content Brief]

[2]. Huo H, et al. Mapping the binding site of the P2X receptor antagonist PPADS reveals the importance of orthosteric site charge and the cysteine-rich head region. J Biol Chem. 2018 Aug 17;293(33):12820-12831. [Content Brief]

[3]. Einfluss von ATP und seinen Derivaten auf die Aktivierung von Monozyten.

[4]. Rost S, et al. P2 receptor antagonist PPADS inhibits mesangial cell proliferation in experimental mesangialproliferative glomerulonephritis. Kidney Int. 2002 Nov;62(5):1659-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.6686 mL	8.3431 mL	16.6861 mL	41.7153 mL
	5 mM	0.3337 mL	1.6686 mL	3.3372 mL	8.3431 mL
	10 mM	0.1669 mL	0.8343 mL	1.6686 mL	4.1715 mL
	15 mM	0.1112 mL	0.5562 mL	1.1124 mL	2.7810 mL
	20 mM	0.0834 mL	0.4172 mL	0.8343 mL	2.0858 mL
	25 mM	0.0667 mL	0.3337 mL	0.6674 mL	1.6686 mL
	30 mM	0.0556 mL	0.2781 mL	0.5562 mL	1.3905 mL
	40 mM	0.0417 mL	0.2086 mL	0.4172 mL	1.0429 mL
	50 mM	0.0334 mL	0.1669 mL	0.3337 mL	0.8343 mL
	60 mM	0.0278 mL	0.1391 mL	0.2781 mL	0.6953 mL
	80 mM	0.0209 mL	0.1043 mL	0.2086 mL	0.5214 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.