2. PPADS tetrasodium

PPADS tetrasodiuma is a non-selective P2X receptor antagonist. PPADS tetrasodiuma blocks recombinant P2X1, -2, -3, -5 with IC50s ranging from 1 to 2.6 μM. PPADS tetrasodiuma blocks native P2Y2-like (IC50~0.9 mM) and recombinant P2Y4 (IC50~15 mM) receptors. PPADS tetrasodiuma is an inhibitor of the reverse mode of the Na/Ca2+ exchanger in guinea pig airway smooth muscle.

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PPADS tetrasodium Chemical Structure

PPADS tetrasodium Chemical Structure

CAS No. : 192575-19-2

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Solid + Solvent
10 mM * 1 mL in Water
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Based on 1 publication(s) in Google Scholar

Other Forms of PPADS tetrasodium:

PPADS tetrasodium Related Antibodies

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Description

PPADS tetrasodiuma is a non-selective P2X receptor antagonist. PPADS tetrasodiuma blocks recombinant P2X1, -2, -3, -5 with IC50s ranging from 1 to 2.6 μM. PPADS tetrasodiuma blocks native P2Y2-like (IC50~0.9 mM) and recombinant P2Y4 (IC50~15 mM) receptors. PPADS tetrasodiuma is an inhibitor of the reverse mode of the Na/Ca2+ exchanger in guinea pig airway smooth muscle[1][2].

In Vitro

PPADS tetrasodiuma (1-30 μM; 10-50 minutes) inhibits Na+/Ca2+ exchanger reverse mode (NCXREV) in a time- and concentration dependent manner[2].
PPADS tetrasodiuma is effective at other native and recombinant P2XRs. At human P2XRs sensitivity to PPADS tetrasodiuma depended on the subtype and is highest at the hP2X1, -2, -3, -5, and -7Rs with an IC50 of ~1-3 and ~30 μM for the hP2X4R[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PPADS tetrasodium Related Antibodies
In Vivo

PPADS tetrasodiuma (15-60 mg/100g body weight (BW); i.p.; every 12 hours for 8 days) inhibits glomerular mesangial cells (MC) proliferation without altering proliferation of non-MC in vivo in mesangial proliferative glomerulonephritis[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley ratsweighing 160 to 200 g (anti-Thy1 disease mode)[4]
Dosage: 15 mg/100g BW, 30 mg/100g BW, 60 mg/100g BW
Administration: i.p.; every 12 hours for 8 days (the first PPADS injection was administered 60 minutes after disease induction, and the loading dose always contained double the amount of PPADS compared to the following injections.)
Result: Specifically and dose-dependently reduced early (day 3) glomerular mesangial cell proliferation without altering proliferation of non-MC.
Molecular Weight

599.30

Formula

C14H10N3Na4O12PS2
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=S(C1=CC=C(/N=N/C2=NC(C)=C(O)C(C=O)=C2COP(O[Na])(O[Na])=O)C(S(=O)(O[Na])=O)=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (83.43 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6686 mL 8.3431 mL 16.6861 mL
5 mM 0.3337 mL 1.6686 mL 3.3372 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥95.0%

COA (0 KB) HNMR (461 KB) MS (229 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.6686 mL 8.3431 mL 16.6861 mL 41.7153 mL
5 mM 0.3337 mL 1.6686 mL 3.3372 mL 8.3431 mL
10 mM 0.1669 mL 0.8343 mL 1.6686 mL 4.1715 mL
15 mM 0.1112 mL 0.5562 mL 1.1124 mL 2.7810 mL
20 mM 0.0834 mL 0.4172 mL 0.8343 mL 2.0858 mL
25 mM 0.0667 mL 0.3337 mL 0.6674 mL 1.6686 mL
30 mM 0.0556 mL 0.2781 mL 0.5562 mL 1.3905 mL
40 mM 0.0417 mL 0.2086 mL 0.4172 mL 1.0429 mL
50 mM 0.0334 mL 0.1669 mL 0.3337 mL 0.8343 mL
60 mM 0.0278 mL 0.1391 mL 0.2781 mL 0.6953 mL
80 mM 0.0209 mL 0.1043 mL 0.2086 mL 0.5214 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PPADS tetrasodium192575-19-2Inhibitorinhibitorinhibit

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