RAD16-I  (Synonyms: RADA16)

RAD16-I (RADA16) is a non-directed self-assembling peptide hydrogel. Under physiological conditions, RAD16-I spontaneously forms a three-dimensional nanofiber network that mimics the extracellular matrix, and possesses excellent properties such as high water content, biocompatibility and degradability. RAD16-I serves as an ideal scaffold for three-dimensional cell culture. RAD16-I not only maintains cell viability and induces self-organization, but also supports cell adhesion, proliferation, differentiation and insulin secretion, effectively stabilizes islet clusters and promotes directed differentiation of the cardiac lineage. RAD16-I can construct a cell-friendly nano-microenvironment for research related to diseases such as myocardial infarction and diabetes^[1][2][3].

The self-assembling peptide hydrogel RAD16-I (0.1-0.3% w/v; up to 21 days) maintains high viability of subATDPCs for up to 21 days, supports basal upregulation of cardiac markers in control medium, enhances the upregulation of cardiac markers when combined with cardiac induction medium, and regulates the viscoelasticity of the scaffold over time^[1].
Modified RAD16-I self-assembling peptide hydrogels (0.15-0.3% w/v) bearing the RGD motif, heparin, or both maintain high viability of subATDPCs, promote cell elongation and orderly arrangement; compared with unmodified RAD16-I hydrogels, they do not significantly alter the expression of myocardial markers; while under cardiomyogenic induction conditions, RAD16-I/RADRGD hydrogels with enhanced initial stiffness downregulate the expression of myocardial proteins in subATDPCs^[1].
RAD16-I self-assembles into β-sheet-containing nanofibers under physiological conditions, with a diameter of 19.58 nm^[3].
The 3D sandwich culture system with RAD16-I maintains the long-term viability of dedifferentiated human ICCs (with a cell survival rate of approximately 85% on day 18 of culture) and also promotes the aggregation of ICCs into larger cell clusters within 18 days, but does not significantly upregulate the expression of β-cell-specific genes compared with the non-adherent control group^[3].
RAD16-I can adsorb onto poly (ethyl acrylate), 90/10 ethyl acrylate-acrylic acid copolymer (mass ratio), and glass coverslip substrates, forming β-sheet structures detectable by Congo Red staining, while unremoved excess peptides form a three-dimensional gel layer with weak adhesion^[4].
RAD16-I (0.025%; 1 h at 37°C) can adsorb and form unique structures on the surfaces of polyethyl acrylate, 90/10 wt% ethyl acrylate-acrylic acid copolymer, and glass coverslip substrates under the conditions of Protocol 2 (no induced self-assembly); among them, the coating supported by 90/10 wt% ethyl acrylate-acrylic acid copolymer is more uniform than that supported by polyethyl acrylate, and the root-mean-square roughness values of the three at a concentration of 0.025% are 0.54 nm, 0.15 nm, and 1.24 nm, respectively^[4].
Adsorption of RAD16-I (0.025-0.1%; 1 h at 37°C, 4 h self-assembly) alters the surface wettability of polyethyl acrylate, ethyl acrylate-acrylic acid copolymer with a mass ratio of 90/10, and glass coverslip substrates, while inducing substrate-specific changes in contact angles and polar components of surface tension; in addition, pre-swelled substrates show a better correlation between wettability and peptide coating uniformity observed by AFM^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RAD16-I (surgical implantation) enables real-time monitoring of myocardial infarct scar formation via electrical impedance spectroscopy, with impedance magnitude changes corresponding to distinct stages of scar development when used to rehydrate a decellularized human pericardium scaffold for implantation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1712.78

C₆₆H₁₁₃N₂₉O₂₅

289042-25-7

Ac-Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala-NH2

Ac-RADARADARADARADA-NH2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Castells-Sala C, et al. Three-Dimensional Cultures of Human Subcutaneous Adipose Tissue-Derived Progenitor Cells Based on RAD16-I Self-Assembling Peptide. Tissue Eng Part C Methods. 2016;22(2):113-124.  [Content Brief]

  [2]. Sanchez B, et al. Towards on line monitoring the evolution of the myocardium infarction scar with an implantable electrical impedance spectrum monitoring system. Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:3223-3226.  [Content Brief]

  [3]. Aloy-Reverté C, et al. Use of RGD-Functionalized Sandwich Cultures to Promote Redifferentiation of Human Pancreatic Beta Cells After In Vitro Expansion. Tissue Eng Part A. 2018;24(5-6):394-406.  [Content Brief]

  [4]. Arnal-Pastor M, et al. Interaction between acrylic substrates and RAD16-I peptide in its self-assembling[J]. Journal of Polymer Research, 2016, 23(9): 173.