2. MAPK/ERK Pathway Apoptosis
  3. Mixed Lineage Kinase RIP kinase
  4. RIPK3-IN-3

RIPK3-IN-3 (compound 20) is a selective inhibitor of RIP kinase RIPK3 (IC50=10 nM). RIPK3 mediates the phosphorylation of Mixed Lineage Kinase (MLKL) and causes necroptosis, while RIPK3-IN-3 inhibits p-MLKL oligomerization and thereby inhibits necroptosis. RIPK3-IN-3 also downregulates CXCL5 secretion and inhibits AsPC-1 cell migration and invasion.

For research use only. We do not sell to patients.

RIPK3-IN-3 Chemical Structure

RIPK3-IN-3 Chemical Structure

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RIPK3-IN-3 Related Antibodies

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Description

RIPK3-IN-3 (compound 20) is a selective inhibitor of RIP kinase RIPK3 (IC50=10 nM). RIPK3 mediates the phosphorylation of Mixed Lineage Kinase (MLKL) and causes necroptosis, while RIPK3-IN-3 inhibits p-MLKL oligomerization and thereby inhibits necroptosis. RIPK3-IN-3 also downregulates CXCL5 secretion and inhibits AsPC-1 cell migration and invasion[1].

IC50 & Target

IC50: 10 nM (RIPK3)[1]
In Vitro

RIPK3-IN-3 Related Antibodies

Western Blot Analysis [1]

Cell Line: HT-29 cell
Concentration: 10, 25, 50 μM
Incubation Time: 2 h
Result: Inhibited phosphorylation of RIPK3 and MLKL in a concentration dependent manner.

Cell Viability Assay [1]

Cell Line: U937 cells, HT-29cell
Concentration: 20,50 μM
Incubation Time: 2 h
Result: Decreased PI-positive cells to Hoechst-positive cells ratio.
Rescued the cell from the TCZ-induced cell necroptosis.

Cell Migration Assay [1]

Cell Line: AsPC-1 cell
Concentration: 20,50 μM
Incubation Time: 24 h
Result: Suppressed migration capability of AsPC-1 pancreatic cancer cells in a dose dependent manner.

Cell Invasion Assay[1]

Cell Line: AsPC-1 cell
Concentration: 20,50 μM
Incubation Time: 2 h
Result: Suppressed invasion capability of AsPC-1 pancreatic cancer cells in a dose dependent manner.
In Vivo
Animal Model: TNF-induced SIRS model (C57BL/6 female mice)[1]
Dosage: 10 or 25 mg/kg, single dose, TNFα (7 μg/mouse, iv)
Administration: Intraperitoneal injection (i.p.)
Result: Protects mice against lethal shock which is induced by TNFα and relieved the TNFα-induced temperature loss in a dose-dependent manner.
Decreases TNFα-induced IL-6 production in the mouse serum.
Molecular Weight

305.36

Formula

C16H11N5S
SMILES

C=CC1=NC=C2N=CN=C(NC3=CC(N=CS4)=C4C=C3)C2=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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RIPK3-IN-3 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RIPK3-IN-3Mixed Lineage KinaseRIP kinaseMLKsReceptor-interacting protein kinasesRIPKprogrammed necrotic cell deathTNFα-induced systemic inflammatory response syndromeHT-29Inhibitorinhibitorinhibit

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