1. Cell Cycle/DNA Damage
    Cytoskeleton
  2. Microtubule/Tubulin
  3. S516

S516 

Cat. No.: HY-130233 Purity: 98.03%
Handling Instructions

S516 (Compound 22) is an active metabolite of CKD-516 and a potent tubulin polymerization inhibitor with an IC50 of 4.29 μM. S516 has marked antitumor activity.

For research use only. We do not sell to patients.

S516 Chemical Structure

S516 Chemical Structure

CAS No. : 1016543-77-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 337 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 550 In-stock
Estimated Time of Arrival: December 31
25 mg USD 990 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1650 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2500 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

S516 (Compound 22) is an active metabolite of CKD-516 and a potent tubulin polymerization inhibitor with an IC50 of 4.29 μM. S516 has marked antitumor activity[1].

IC50 & Target

IC50: 4.29 μM (tubulin polymerization)[1]

In Vitro

S516 has potent cytotoxicity with IC50s of 4.8 nM, 42.8 nM and 24.9 nM for HL-60, HCT116 and HCT15 cells, respectively[1].
S516 (Compound 22; 30 nM; 16 hours; HL60 cells) treatmemt causes significant arrest of cells at the G2/M phase, resulting in apoptosis with concomitant loss of G0/G1 phase[1].

Cell Cycle Analysis[1]

Cell Line: HL60 cells
Concentration: 30 nM
Incubation Time: 16 hours
Result: Caused significant arrest of cells at the G2/M phase, resulting in apoptosis with concomitant loss of G0/G1 phase.
In Vivo

S516 (Compound 22; 5-10 mg/kg; intraperitoneal injection; mice) treatment has promising antitumor activity (inhibition ratio (IR)> 63%) in human LX-1 lung cancer and CX-1 colon cancer mouse xenografts[1].

Animal Model: Mice bearing 3LL lung cancer[1]
Dosage: 5 mg/kg, 10 mg/kg
Administration: Intraperitoneal injection
Result: Had promising antitumor activity (inhibition ratio (IR)> 63%).
Molecular Weight

437.47

Formula

C₂₁H₁₉N₅O₄S

CAS No.

1016543-77-3

SMILES

O=C(C1=CC=C(C2=CSC(N)=N2)C=C1N3N=CN=C3)C4=CC(OC)=C(OC)C(OC)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (28.57 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2859 mL 11.4294 mL 22.8587 mL
5 mM 0.4572 mL 2.2859 mL 4.5717 mL
10 mM 0.2286 mL 1.1429 mL 2.2859 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.25 mg/mL (2.86 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

S516S 516S-516Microtubule/TubulinInhibitorinhibitorinhibit

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S516
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HY-130233
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