AXKO-0046
Based on 7 publication(s) in Google Scholar
AXKO-0046, indole derivative, is an uncompetitive Lactate dehydrogenase B (LDHB) selective inhibitor.AXKO-0046 has LDHB inhibitory activity with an EC50 value of 42 nM.AXKO-0046 can be used for the research of cancer metabolism.
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 3103525-82-9
- Formula: C25H33N3
- Molecular Weight:375.55
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-L092
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Metabolic Enzyme Compound Library
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
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High-Throughput Bioactive Compound Library
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Lactylation Compound Library
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Metabolism/Protease Compound Library
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Anti-Cancer Compound Library
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Publications Citing Use of MedChemExpress (MCE) AXKO-0046
More- Mol Neurodegener. 2026 May 23. [Abstract]
- Nat Commun. 2025 Jan 27;16(1):1083. [Abstract]
- Cell Rep. 2024 Apr 23;43(4):114047. [Abstract]
- Pharmaceutics. 2023 Oct 1;15(10):2411. [Abstract]
- J Pathol. 2025 Oct 23. [Abstract]
- FASEB J. 2026 May 31;40(10):e71888. [Abstract]
- bioRxiv. 2025 Jan 18:2025.01.13.632864. [Abstract]
Biological Activity
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LDHB 42 nM (EC50) |
AXKO-0046 has LDHB inhibitory activity with an EC50 value of 42 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3103525-82-9
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Appearance Oil
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Molecular Weight 375.55
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Formula C25H33N3
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Color Light yellow to yellow
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SMILES
C1(NCC(N2)=C(CCNCC3=CC=CC=C3)C4=C2C=CC=C4)CCCCCC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (7)
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Journal Impact Factor
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Most Recent
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Mol Neurodegener
In vivo profiling of astrocyte secretome reveals brain-region specific regulatory networks in a mouse model of amyloid pathology. [Abstract]2026 May 23. PMID: 42177534 -
Nat Commun
MCT1 lactate transporter blockade re-invigorates anti-tumor immunity through metabolic rewiring of dendritic cells in melanoma. [Abstract]2025 Jan 27;16(1):1083. PMID: 39870647 -
Cell Rep
LDHB contributes to the regulation of lactate levels and basal insulin secretion in human pancreatic β cells. [Abstract]2024 Apr 23;43(4):114047. PMID: 38607916 -
Pharmaceutics
Targeting Lactate Dehydrogenase-B as a Strategy to Fight Cancer: Identification of Potential Inhibitors by In Silico Analysis and In Vitro Screening. [Abstract]2023 Oct 1;15(10):2411. PMID: 37896171 -
J Pathol
Lactate-mediated activation of GPR81 regulates BCR/Abl protein expression in chronic myeloid leukemia cells selected under low oxygen tension. [Abstract]2025 Oct 23. PMID: 41128634 -
FASEB J
Maternal LDHB Safeguards Redox Balance and Developmental Competence During Preimplantation Embryo Cleavage. [Abstract]2026 May 31;40(10):e71888. PMID: 42117986 -
bioRxiv
Fluorescence lifetime imaging microscopy for metabolic analysis of LDHB inhibition in triple negative breast cancer. [Abstract]2025 Jan 18:2025.01.13.632864. PMID: 39868164
Solvent & Solubility
DMSO : 100 mg/mL (266.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6628 mL | 13.3138 mL | 26.6276 mL | 66.5690 mL |
| 5 mM | 0.5326 mL | 2.6628 mL | 5.3255 mL | 13.3138 mL | |
| 10 mM | 0.2663 mL | 1.3314 mL | 2.6628 mL | 6.6569 mL | |
| 15 mM | 0.1775 mL | 0.8876 mL | 1.7752 mL | 4.4379 mL | |
| 20 mM | 0.1331 mL | 0.6657 mL | 1.3314 mL | 3.3285 mL | |
| 25 mM | 0.1065 mL | 0.5326 mL | 1.0651 mL | 2.6628 mL | |
| 30 mM | 0.0888 mL | 0.4438 mL | 0.8876 mL | 2.2190 mL | |
| 40 mM | 0.0666 mL | 0.3328 mL | 0.6657 mL | 1.6642 mL | |
| 50 mM | 0.0533 mL | 0.2663 mL | 0.5326 mL | 1.3314 mL | |
| 60 mM | 0.0444 mL | 0.2219 mL | 0.4438 mL | 1.1095 mL | |
| 80 mM | 0.0333 mL | 0.1664 mL | 0.3328 mL | 0.8321 mL | |
| 100 mM | 0.0266 mL | 0.1331 mL | 0.2663 mL | 0.6657 mL |
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