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Cu2 -selective probe

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Fluorescent Dye (3)
Click Chemistry:	Azide (1)

Inhibitors & Agonists

Fluorescent Dyes

Click Chemistry

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D1601

N-Aminofluorescein 1 Publications Verification	Fluorescent Dye	Others
N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu ²⁺. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu ²⁺ in biological systems with λex/em=495/516 nm . N-Aminofluorescein can be used to measure the concentration of copper ions in cells .

HY-D3216

ER-Cu(II)	Fluorescent Dye	Others
ER-Cu (II) is an endoplasmic reticulum-selective fluorescent Cu ²⁺ probe. ER-Cu (II) localizes specifically to the endoplasmic reticulum of hepatocytes .

HY-D3152

NRh-Cu	Fluorescent Dye	Others
NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and *Cu ²⁺*-selective probe (λ_ex=680 nm, λ_em=740 nm). When NRh-Cu interacts with Cu ²⁺, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu ²⁺ imaging in live cells and in vivo .

Cat. No.	Product Name	Type

HY-D1601

N-Aminofluorescein 1 Publications Verification	Fluorescent Dyes
N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu ²⁺. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu ²⁺ in biological systems with λex/em=495/516 nm . N-Aminofluorescein can be used to measure the concentration of copper ions in cells .

N-Aminofluorescein

1 Publications Verification

Fluorescent Dyes

N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu ²⁺. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu ²⁺ in biological systems with λex/em=495/516 nm . N-Aminofluorescein can be used to measure the concentration of copper ions in cells .

HY-D3216

ER-Cu(II)	Fluorescent Dyes
ER-Cu (II) is an endoplasmic reticulum-selective fluorescent Cu ²⁺ probe. ER-Cu (II) localizes specifically to the endoplasmic reticulum of hepatocytes .

HY-D3152

NRh-Cu	Fluorescent Dyes
NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and *Cu ²⁺*-selective probe (λ_ex=680 nm, λ_em=740 nm). When NRh-Cu interacts with Cu ²⁺, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu ²⁺ imaging in live cells and in vivo .

NRh-Cu

Fluorescent Dyes

NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu ²⁺-selective probe (λ_ex=680 nm, λ_em=740 nm). When NRh-Cu interacts with Cu ²⁺, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu ²⁺ imaging in live cells and in vivo .

Cat. No.	Product Name		Classification

HY-D3216

ER-Cu(II)		Azide
ER-Cu (II) is an endoplasmic reticulum-selective fluorescent Cu ²⁺ probe. ER-Cu (II) localizes specifically to the endoplasmic reticulum of hepatocytes .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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