N-Aminofluorescein

Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
N-Aminofluorescein consists of a fluorescein moiety and a hydrazide group, which can recognize and bind Cu²⁺ and promote amide hydrolysis^[1].
N-Aminofluorescein is selective for Cu²⁺, showing absorption and emission bands at 632 nm and 515 nm, respectively, in 70% HEPES buffered aqueous solution (pH 7.4) containing Cu^2+[2].
Guide (The following is our recommended protocol. This protocol is provided as a guideline and should be modified according to your specific needs).
General procedure for Cu²⁺ determination^[1]:
1. Prepare a 1 mM stock solution of spirolactam fluorescein hydrazide in ethanol;
2. Perform the fluorescence turn-on reaction in 0.01 M Tris-HCl buffer (pH 7.2) by adding 10 μM N-Aminofluorescein;
3. Add an appropriate amount of sample solution so that the final concentration of Cu²⁺ does not exceed 10 μM, and adjust the final volume to 10 mL with 0.01 M Tris-HCl buffer (pH 7.2);
4. After 2 h, transfer 3 mL of the solution into a 1 cm quartz cuvette, and measure at room temperature with λex/em = 495/516 nm and excitation and emission slit widths of 5 nm;
5. Prepare a blank solution without Cu²⁺ and measure under the same conditions for comparison.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.