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  3. Fluorescent Dye
  4. NRh-Cu

NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu2+-selective probe (λex=680 nm, λem=740 nm). When NRh-Cu interacts with Cu2+, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu2+ imaging in live cells and in vivo.

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NRh-Cu

NRh-Cu Chemical Structure

CAS No. : 2361979-91-9

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NRh-Cu Related Antibodies

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Description

NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu2+-selective probe (λex=680 nm, λem=740 nm). When NRh-Cu interacts with Cu2+, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu2+ imaging in live cells and in vivo[1].

In Vitro

NRh-Cu (10 μM) exhibits high selectivity for Cu2+ over a wide range of other metal ions in ethanol/H2O solution, with a rapid fluorescence turn-on response reaching maximum intensity within 10 min; with a fluorescence-based detection limit of 0.95 ppb and absorption-based detection limit of 6.2 ppb[1].
NRh-Cu (2-80 μM; 48 h) shows no significant cytotoxicity toward HeLa cells[1].
NRh-Cu (10 μM; 1 h) exhibits good membrane permeability in HeLa cells and generates strong fluorescence in the presence of intracellular Cu2+[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NRh-Cu Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HeLa cells
Concentration: 2-80 μM
Incubation Time: 48 h
Result: Showed no significant difference in HeLa cell proliferation after incubation at concentrations from 2 to 80 μM for 48 h.
In Vivo

NRh-Cu (0.2 mL, 0.2 mg/mL; intravenous injection; single dose) binds to systemic Cu2+ in living mice, and the in vivo near-infrared fluorescence emission intensity increases significantly[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Kunming mice (male, 4 weeks old, ~20 g)[1]
Dosage: 0.2 mL, 0.2 mg/mL
Administration: i.v.; single dose
Result: Showed low fluorescence emission intensity when administered alone.
Showed a significant increase in fluorescence emission intensity when followed by systemic Cu2+ administration compared to the NRh-Cu-only group.
Molecular Weight

761.99

Formula

C49H55N5O3
CAS No.
SMILES

CCN(C1=CC=C2C(OC3=C(C24C5=C(C(N4/N=C/C6=CC=C(C=C6O)N(CC)CC)=O)C=CC=C5)CCC/C3=C\C=C7N(C8=C(C/7(C)C)C=CC=C8)CC)=C1)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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NRh-Cu Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NRh-Cu2361979-91-9Fluorescent Dyeliving cellsethanol/H2O solutionintracellular Cu2+ imagingliving micecolorimetric probeCu2+in vivo Cu2+ imagingnear-infrared fluorescent turn-on indicatorCu2+-selective probeHeLa cells
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