NRh-Cu
NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu2+-selective probe (λex=680 nm, λem=740 nm). When NRh-Cu interacts with Cu2+, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu2+ imaging in live cells and in vivo.
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- CAS No.: 2361979-91-9
- Formule: C49H55N5O3
- Masse moléculaire:761.99
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
NRh-Cu (10 μM) exhibits high selectivity for Cu2+ over a wide range of other metal ions in ethanol/H2O solution, with a rapid fluorescence turn-on response reaching maximum intensity within 10 min; with a fluorescence-based detection limit of 0.95 ppb and absorption-based detection limit of 6.2 ppb[1].
NRh-Cu (2-80 μM; 48 h) shows no significant cytotoxicity toward HeLa cells[1].
NRh-Cu (10 μM; 1 h) exhibits good membrane permeability in HeLa cells and generates strong fluorescence in the presence of intracellular Cu2+[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:2-80 μM
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Incubation Time:48 h
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Result:Showed no significant difference in HeLa cell proliferation after incubation at concentrations from 2 to 80 μM for 48 h.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Kunming mice (male, 4 weeks old, ~20 g)[1]
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Dosage:0.2 mL, 0.2 mg/mL
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Administration:i.v.; single dose
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Result:Showed low fluorescence emission intensity when administered alone.
Showed a significant increase in fluorescence emission intensity when followed by systemic Cu2+ administration compared to the NRh-Cu-only group.
Chemical Information
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CAS No. 2361979-91-9
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Masse moléculaire 761.99
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Formule C49H55N5O3
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SMILES
CCN(C1=CC=C2C(OC3=C(C24C5=C(C(N4/N=C/C6=CC=C(C=C6O)N(CC)CC)=O)C=CC=C5)CCC/C3=C\C=C7N(C8=C(C/7(C)C)C=CC=C8)CC)=C1)CC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NRh-Cu Related Classifications
HY-D3152 Related Classifications
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