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SDS/PAGE

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By Targets:	Biochemical Assay Reagents (2) Environmental Pollutants (2) Fluorescent Dye (4)

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

MCE Kits

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D0232

Brilliant Blue R250 1 Publications Verification Brilliant Blue R	Environmental Pollutants Fluorescent Dye	Others
Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels .

HY-NP002

BSA Standard Solution (5 mg/mL)	Biochemical Assay Reagents	Others
BSA Standard Solution (5 mg/mL) is a bovine serum albumin solution commonly used as a standard for total serum protein detection. BSA Standard Solution (5 mg/mL) can be used for drawing standard curves and calibrating experiments in total protein assays. BSA Standard Solution (5 mg/mL) is also suitable for various biological experiments, including PCR detection inhibition, antibody desalting, chromatography control, SDS-PAGE electrophoresis, and calibration of UV spectrophotometers .

HY-D0006

Bathophenanthroline	Fluorescent Dye	Others
Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru ²⁺. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].

HY-D1590

BODIPY Green 8-P2M	Fluorescent Dye	Others
ODIPY Green 8-P2M is a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore, the fluorescence is strongly quenched by d-PeT and then can be restored after reaction with thiol, resulting in an extremely high signal-to-noise ratio. ODIPY Green 8-P2M can be useful for detecting extremely low concentrations of protein in the gel after SDS-PAGE .

HY-D0014

Brilliant blue G-250 3 Publications Verification	Environmental Pollutants Biochemical Assay Reagents	Others
Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

HY-Y0700

Calconcarboxylic acid	Fluorescent Dye	Others
Calconcarboxylic acid, an azo dye, acts as a silver-ion sensitizer to stain protein in SDS-PAGE gels. Calconcarboxylic acid increases silver binding on protein bands or spots by the formation of a silver-dye complex and also increases the reducing power o

Cat. No.	Product Name	Type

HY-D0232

Brilliant Blue R250 1 Publications Verification Brilliant Blue R	Fluorescent Dyes
Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels .

HY-D0006

Bathophenanthroline	Fluorescent Dyes
Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru ²⁺. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].

Bathophenanthroline

Fluorescent Dyes

Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru ²⁺. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].

HY-D0014

Brilliant blue G-250 3 Publications Verification	Fluorescent Dyes
Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

Brilliant blue G-250

3 Publications Verification

Fluorescent Dyes

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

HY-Y0700

Calconcarboxylic acid	Fluorescent Dyes
Calconcarboxylic acid, an azo dye, acts as a silver-ion sensitizer to stain protein in SDS-PAGE gels. Calconcarboxylic acid increases silver binding on protein bands or spots by the formation of a silver-dye complex and also increases the reducing power o

Cat. No.	Product Name	Type

HY-D0232

Brilliant Blue R250 1 Publications Verification Brilliant Blue R	Biochemical Assay Reagents
Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels .

HY-NP002

BSA Standard Solution (5 mg/mL)	Biochemical Assay Reagents
BSA Standard Solution (5 mg/mL) is a bovine serum albumin solution commonly used as a standard for total serum protein detection. BSA Standard Solution (5 mg/mL) can be used for drawing standard curves and calibrating experiments in total protein assays. BSA Standard Solution (5 mg/mL) is also suitable for various biological experiments, including PCR detection inhibition, antibody desalting, chromatography control, SDS-PAGE electrophoresis, and calibration of UV spectrophotometers .

BSA Standard Solution (5 mg/mL)

Biochemical Assay Reagents

BSA Standard Solution (5 mg/mL) is a bovine serum albumin solution commonly used as a standard for total serum protein detection. BSA Standard Solution (5 mg/mL) can be used for drawing standard curves and calibrating experiments in total protein assays. BSA Standard Solution (5 mg/mL) is also suitable for various biological experiments, including PCR detection inhibition, antibody desalting, chromatography control, SDS-PAGE electrophoresis, and calibration of UV spectrophotometers .

HY-D0014

Brilliant blue G-250 3 Publications Verification	Biochemical Assay Reagents
Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome .

Brilliant blue G-250

3 Publications Verification

Biochemical Assay Reagents

Cat. No.	Product Name

HY-K1020

Tris-Glycine-SDS Powder (1 L of 1×)
MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1100

SDS-PAGE Sample Loading Buffer (4×) 3 Publications Verification
SDS-PAGE Sample Loading Buffer (4×) is appropriate for loading protein samples on SDS-PAGE. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1108

SDS-PAGE Protein Loading Buffer (non-Reducing, 5×)
MCE SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) is appropriate for loading protein samples on SDS-PAGE. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1103

Coomassie Blue Fast Staining Solution (No Heating, 10×) 1 Publications Verification
MCE Coomassie Blue Fast Staining Solution (No Heating, 10×) uses Coomassie Brilliant Blue G250 as the dye. It can be used for rapid, sensitive staining of protein gels in SDS-PAGE or Native-PAGE without contamination, or for detecting residual proteins on PAGE gels after Western Blot transfer. The 25 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Coomassie Blue Fast Staining Solution (No Heating, 10×)

1 Publications Verification

MCE Coomassie Blue Fast Staining Solution (No Heating, 10×) uses Coomassie Brilliant Blue G250 as the dye. It can be used for rapid, sensitive staining of protein gels in SDS-PAGE or Native-PAGE without contamination, or for detecting residual proteins on PAGE gels after Western Blot transfer. The 25 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1101

3-Color Prestained Protein Marker (2.7-40 kDa)
MCE 3-Color Prestained Protein Marker (2.7-40 kDa) is a three-color protein standard with 9 prestained proteins ranging from 2.7 kDa to 40 kDa. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1030

SDS dust-free Powder 1 Publications Verification
MCE SDS dust-free Powder can be directly prepared into 10% SDS solution, and is widely used for protein denaturation.

HY-K1019

Tris-MOPS-SDS Powder (1 L of 1×)
MCE Tris-MOPS-SDS Powder (1 L of 1×) consists of Tris, MOPS, EDTA and SDS, and is mainly used for separation of medium- to large-sized proteins. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1018

Tris-MES-SDS Powder (1 L of 1×)
MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS, and is mainly used for separation of small- to medium-sized proteins. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

HY-K1011

3-Color Prestained Protein Marker (10-190 kDa) 4 Publications Verification
MCE 3-Color Prestained Protein Marker (10-190 kDa) is a three-color protein standard with 10 prestained proteins ranging from 10 kDa to 190 kDa. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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