Calconcarboxylic acid
Based on 1 Customer Validation
Calconcarboxylic acid, an azo dye, acts as a silver-ion sensitizer to stain protein in SDS-PAGE gels. Calconcarboxylic acid increases silver binding on protein bands or spots by the formation of a silver-dye complex and also increases the reducing power o
For research use only. We do not sell to patients.
- CAS No.: 3737-95-9
- Formula: C21H14N2O7S
- Molecular Weight:438.41
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Storage:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BaF3 | EC50 |
10 μM
Compound: 2i
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Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
Effective concentration for thrombopoietin luciferase activity was determined in BAF-3 cells
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[PMID: 11606138] |
Chemical Information
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CAS No. 3737-95-9
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Appearance Solid
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Molecular Weight 438.41
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Formula C21H14N2O7S
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Color Brown to black
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SMILES
O=C(C1=C(O)C(/N=N/C2=C3C=CC=CC3=C(S(=O)(O)=O)C=C2O)=C4C=CC=CC4=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 125 mg/mL (285.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Jin LT, et al. Sensitive silver staining of protein in sodium dodecyl sulfate-polyacrylamide gels using an azo dye, calconcarboxylic acid, as a silver-ion sensitizer. Electrophoresis. 2004 Aug;25(15):2494-500. [Content Brief]
[2]. Hong HY, et al. Detection of proteins on polyacrylamide gels using calconcarboxylic acid. Anal Biochem. 1993 Oct;214(1):96-9. [Content Brief]
[3]. Li-Tai Jin, et al. Sensitive silver staining of protein in sodium dodecyl sulfate-polyacrylamide gels using an azo dye, calconcarboxylic acid, as a silver-ion sensitizer. Electrophoresis. 2004 Aug;25(15):2494-500. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2810 mL | 11.4048 mL | 22.8097 mL | 57.0242 mL |
| 5 mM | 0.4562 mL | 2.2810 mL | 4.5619 mL | 11.4048 mL | |
| 10 mM | 0.2281 mL | 1.1405 mL | 2.2810 mL | 5.7024 mL | |
| 15 mM | 0.1521 mL | 0.7603 mL | 1.5206 mL | 3.8016 mL | |
| 20 mM | 0.1140 mL | 0.5702 mL | 1.1405 mL | 2.8512 mL | |
| 25 mM | 0.0912 mL | 0.4562 mL | 0.9124 mL | 2.2810 mL | |
| 30 mM | 0.0760 mL | 0.3802 mL | 0.7603 mL | 1.9008 mL | |
| 40 mM | 0.0570 mL | 0.2851 mL | 0.5702 mL | 1.4256 mL | |
| 50 mM | 0.0456 mL | 0.2281 mL | 0.4562 mL | 1.1405 mL | |
| 60 mM | 0.0380 mL | 0.1901 mL | 0.3802 mL | 0.9504 mL | |
| 80 mM | 0.0285 mL | 0.1426 mL | 0.2851 mL | 0.7128 mL | |
| 100 mM | 0.0228 mL | 0.1140 mL | 0.2281 mL | 0.5702 mL |
Calconcarboxylic acid Related Classifications
HY-Y0700 Related Classifications
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