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  2. Fluorescent Dye
  3. BODIPY Green 8-P2M

ODIPY Green 8-P2M is a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore, the fluorescence is strongly quenched by d-PeT and then can be restored after reaction with thiol, resulting in an extremely high signal-to-noise ratio. ODIPY Green 8-P2M can be useful for detecting extremely low concentrations of protein in the gel after SDS-PAGE.

For research use only. We do not sell to patients.

BODIPY Green 8-P2M Chemical Structure

BODIPY Green 8-P2M Chemical Structure

CAS No. : 929679-22-1

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5 mg USD 620 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

ODIPY Green 8-P2M is a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore, the fluorescence is strongly quenched by d-PeT and then can be restored after reaction with thiol, resulting in an extremely high signal-to-noise ratio. ODIPY Green 8-P2M can be useful for detecting extremely low concentrations of protein in the gel after SDS-PAGE[1].

Molecular Weight

419.23

Formula

C23H20BF2N3O2

CAS No.
Appearance

Solid

Color

Brown to red

SMILES

O=C1C=CC(N1C2=C(C3=C4C(C)=CC(C)=[N]4[B+3]([F-])([N-]5C(C)=CC(C)=C35)[F-])C=CC=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (119.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3853 mL 11.9266 mL 23.8533 mL
5 mM 0.4771 mL 2.3853 mL 4.7707 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3853 mL 11.9266 mL 23.8533 mL 59.6331 mL
5 mM 0.4771 mL 2.3853 mL 4.7707 mL 11.9266 mL
10 mM 0.2385 mL 1.1927 mL 2.3853 mL 5.9633 mL
15 mM 0.1590 mL 0.7951 mL 1.5902 mL 3.9755 mL
20 mM 0.1193 mL 0.5963 mL 1.1927 mL 2.9817 mL
25 mM 0.0954 mL 0.4771 mL 0.9541 mL 2.3853 mL
30 mM 0.0795 mL 0.3976 mL 0.7951 mL 1.9878 mL
40 mM 0.0596 mL 0.2982 mL 0.5963 mL 1.4908 mL
50 mM 0.0477 mL 0.2385 mL 0.4771 mL 1.1927 mL
60 mM 0.0398 mL 0.1988 mL 0.3976 mL 0.9939 mL
80 mM 0.0298 mL 0.1491 mL 0.2982 mL 0.7454 mL
100 mM 0.0239 mL 0.1193 mL 0.2385 mL 0.5963 mL
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BODIPY Green 8-P2M Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BODIPY Green 8-P2M
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HY-D1590
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