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protoplasts

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By Targets:	Antibiotic (1) Biochemical Assay Reagents (5) Endogenous Metabolite (1) Herbicide (2)
By Research Areas:	Cancer (2) Infection (2)

Inhibitors & Agonists

Biochemical Assay Reagents

Natural
Products

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-B2220A

Cellulase R-10 from Trichoderma Vride	Endogenous Metabolite	Others
Cellulase R-10 from Trichoderma Vride is an enzyme mixture that includes cellulase, hemicellulase, pectinase and protease, etc. Cellulase R-10 from Trichoderma Vride can act on the 1,4-β-D-glucosidic bonds in cellulose, catalyzing the hydrolysis of cellulose molecules and generating cellulose oligosaccharides, cellulose di-saccharides and glucose. Cellulase R-10 from Trichoderma Vride can be used in combination with lyase R-10 to break down the cell walls of plants, preparing protoplasts. Cellulase R-10 from Trichoderma Vride is widely applied in plant cell hybridization research .

HY-126224

Driselase, Basidiomycetes sp	Biochemical Assay Reagents	Others
Driselase, Basidiomycetes sp, a complex mixt. of wall-digesting enzymes, is a specific commercial fungal protoplasting enzyme preparation. Driselase can be used in combination with lyase to promote protoplast formation in fungi. Driselase is by far the most potent of the enzymes tested for polysaccharide digestion and greatly increases both tensile and indentation compliances, yet it does not induce wall creep, even after 6 h of digestion .

HY-E70396

Pectolyase Y-23, A. japonicus	Biochemical Assay Reagents	Others
Pectolyase Y-23, A. japonicus is an enzyme derived from Aspergillus japonicas. Pectolyase Y-23, A. japonicus can be used in combination with cellulase to isolate bioactive protoplasts .

HY-172190

MS Medium	Biochemical Assay Reagents	Others
MS Medium is a basic medium for plant tissue culture. MS Medium has a high concentration of inorganic salts and is suitable for plant protoplast, cell and tissue culture .

HY-179187

NT Medium	Biochemical Assay Reagents	Others
NT Medium is a balanced culture medium mainly used for tobacco protoplast culture.

HY-179188

DPD Medium	Biochemical Assay Reagents	Others
DPD Medium is mainly used for tissue culture and protoplast culture of woody plants, especially trees.

HY-N14222

Epopromycin A	Antibiotic Herbicide	Infection Cancer
Epopromycin A inhibits the cell wall synthesis of lettuce protoplasts, thereby exhibiting herbicidal activity. Epopromycin A exhibits cytotoxicity in B16 melanoma cell with an IC₅₀ of 0.003 µg/mL .

HY-N14236

Epopromycin B	Herbicide	Infection Cancer
Epopromycin B inhibits the cell wall synthesis of lettuce protoplasts, thereby exhibiting herbicidal activity. Epopromycin B exhibits cytotoxicity in B16 melanoma cell with an IC₅₀ of 0.003 µg/mL .

MS Medium	Biochemical Assay Reagents
MS Medium is a basic medium for plant tissue culture. MS Medium has a high concentration of inorganic salts and is suitable for plant protoplast, cell and tissue culture .

NT Medium	Biochemical Assay Reagents
NT Medium is a balanced culture medium mainly used for tobacco protoplast culture.

DPD Medium	Biochemical Assay Reagents
DPD Medium is mainly used for tissue culture and protoplast culture of woody plants, especially trees.

Epopromycin A	Microorganisms Antibiotics Other Antibiotics Source Classification	Antibiotic Herbicide
Epopromycin A inhibits the cell wall synthesis of lettuce protoplasts, thereby exhibiting herbicidal activity. Epopromycin A exhibits cytotoxicity in B16 melanoma cell with an IC₅₀ of 0.003 µg/mL .

Epopromycin B	Microorganisms Antibiotics Other Antibiotics Source Classification	Herbicide
Epopromycin B inhibits the cell wall synthesis of lettuce protoplasts, thereby exhibiting herbicidal activity. Epopromycin B exhibits cytotoxicity in B16 melanoma cell with an IC₅₀ of 0.003 µg/mL .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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protoplasts

Inhibitors & Agonists

Biochemical Assay Reagents

NaturalProducts

Natural
Products