Strophanthidin
Based on 2 publication(s) in Google Scholar
Strophanthidin is a naturally available cardiac glycoside. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 μmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal. Strophanthidin increases both diastolic and systolic intracellular Ca2+ concentration.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.71%
- CAS No.: 66-28-4
- 화학식: C23H32O6
- 분자량:404.50
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Strophanthidin
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Biological Activity
Na+/K+-ATPase[2]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 1A9 | ED50 |
0.15 μg/mL
Compound: 19
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Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human 1A9 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| A549 | ED50 |
0.11 μg/mL
Compound: 19
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Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| CAKI-1 | ED50 |
0.14 μg/mL
Compound: 19
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Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human CAKI-1 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| KB | ED50 |
0.44 μg/mL
Compound: strophanthidin
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 4031899] |
| KB | ED50 |
0.61 μg/mL
Compound: 19
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Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| MCF7 | ED50 |
0.38 μg/mL
Compound: 19
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Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| PC-3 | ED50 |
0.17 μg/mL
Compound: 19
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Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| SK-MEL-2 | ED50 |
0.13 μg/mL
Compound: 19
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Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
| U-251 | EC50 |
2 μM
Compound: 10
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Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay
Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid under hypoxic condition after 16 to 24 hrs by luciferase reporter gene assay
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[PMID: 19405508] |
| U-87MG ATCC | ED50 |
0.72 μg/mL
Compound: 19
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Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay
Cytotoxicity against human U87MG cells assessed as growth inhibition after 72 hrs by SRB assay
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[PMID: 24582402] |
Strophanthidin (0~10 μM; 24 hours; MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells[1].
Strophanthidin (0.5 to 500 μM; PBMCs) does not show significant cytotoxicity in PBMCs. Strophanthidin (2 μM; MCF-7 cells) can arrest cell cycle at the G2/M phase[1].
Strophanthidin (MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells. Strophanthidin (MCF-7, A549, and HepG2 cells) inhibits the expression of checkpoint and cyclin-dependent kinases in three cancer cells compared to untreated controls. Strophanthidin can modulate the protein localization from the nucleus to the membrane as well as to the cytoplasm. Strophanthidin is a monosaccharide cardiac glycoside with one aglycone portion and without any sugar unit. Strophanthidin induces apoptosis by the attenuation of multiple biochemical signaling pathways and by arresting cell cycle at the G2/M phase through p53-dependent and p53-independent mechanisms[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, A549, and HepG2 cells
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Concentration:0~10 μM
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Incubation Time:24 hours
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Result:Inhibited the proliferation in three different cancer cells.
Chemical Information
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CAS No. 66-28-4
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Appearance Solid
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분자량 404.50
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화학식 C23H32O6
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Color White to off-white
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SMILES
O=C[C@@]12[C@]3([H])[C@](CC[C@@]1(C[C@@H](O)CC2)O)([H])[C@@]4([C@@](C)([C@@H](C5=CC(OC5)=O)CC4)CC3)O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
용액&용해도
Ethanol : 25 mg/mL (61.80 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
순도&문서
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Data Sheet (282 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Reddy D, et al. Strophanthidin Attenuates MAPK, PI3K/AKT/mTOR, and Wnt/β-Catenin Signaling Pathways in Human Cancers. Front Oncol. 2020;9:1469. Published 2020 Jan 17. [Content Brief]
[2]. Su SW, et al. Relationship between cardiotonic effects and inhibition on cardiac sarcolemmal Na+,K+-ATPase of strophan-thidin at low concentrations. Acta Pharmacol Sin. 2003;24(11):1103-1107. [Content Brief]
[3]. Bennett DL, et al. Strophanthidin-induced gain of Ca2+ occurs during diastole and not systole in guinea-pig ventricular myocytes. Pflugers Arch. 1999;437(5):731-736. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.4722 mL | 12.3609 mL | 24.7219 mL | 61.8047 mL |
| 5 mM | 0.4944 mL | 2.4722 mL | 4.9444 mL | 12.3609 mL | |
| 10 mM | 0.2472 mL | 1.2361 mL | 2.4722 mL | 6.1805 mL | |
| 15 mM | 0.1648 mL | 0.8241 mL | 1.6481 mL | 4.1203 mL | |
| 20 mM | 0.1236 mL | 0.6180 mL | 1.2361 mL | 3.0902 mL | |
| 25 mM | 0.0989 mL | 0.4944 mL | 0.9889 mL | 2.4722 mL | |
| 30 mM | 0.0824 mL | 0.4120 mL | 0.8241 mL | 2.0602 mL | |
| 40 mM | 0.0618 mL | 0.3090 mL | 0.6180 mL | 1.5451 mL | |
| 50 mM | 0.0494 mL | 0.2472 mL | 0.4944 mL | 1.2361 mL | |
| 60 mM | 0.0412 mL | 0.2060 mL | 0.4120 mL | 1.0301 mL |
Strophanthidin Related Classifications
HY-114252 Related Classifications
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